GapMind for catabolism of small carbon sources

 

Finding step acs for acetate catabolism in Paraburkholderia bryophila 376MFSha3.1

5 candidates for acs: acetyl-CoA synthetase, AMP-forming

Score Gene Description Similar to Id. Cov. Bits Other hit Other id. Other bits
hi H281DRAFT_04953 acetyl-coenzyme A synthetase Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 (characterized) 80% 100% 1120.9
hi H281DRAFT_04953 acetyl-coenzyme A synthetase acs: acetate--CoA ligase (EC 6.2.1.1) (TIGR02188) 100% 971.9
med H281DRAFT_05523 propionyl-CoA synthetase (EC 6.2.1.17) Acyl-CoA synthetase short-chain family member 3, mitochondrial; Acetate--CoA ligase 3; Acyl-CoA synthetase short-chain family member 3; Propionate--CoA ligase; EC 6.2.1.1; EC 6.2.1.17 (characterized) 50% 90% 620.5 Propionate--CoA ligase (EC 6.2.1.17) 50% 647.9
med H281DRAFT_05558 fatty-acyl-CoA synthase isovalerate--CoA ligase (EC 6.2.1.1) (characterized) 47% 95% 506.5 3-(methylthio)propionyl-CoA ligase (EC 6.2.1.44) 53% 576.2
med H281DRAFT_04471 propionyl-CoA synthetase Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; EC 6.2.1.1; Acetate--CoA ligase; Acyl-activating enzyme (uncharacterized) 42% 94% 504.2 propionate-CoA ligase (EC 6.2.1.17) 62% 801.2
med H281DRAFT_01203 acetyl-CoA synthetase 4-hydroxybutyrate-CoA ligase (EC 6.2.1.40) (characterized) 40% 94% 368.2 Acyl-coenzyme A synthetase ACSM3, mitochondrial; Acyl-CoA synthetase medium-chain family member 3; Butyrate--CoA ligase 3; Butyryl-coenzyme A synthetase 3; Middle-chain acyl-CoA synthetase 3; Propionate--CoA ligase; Protein SA homolog; EC 6.2.1.2; EC 6.2.1.17 37% 364.0

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

Also see fitness data for the candidates

Definition of step acs

Or cluster all characterized acs proteins

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory