GapMind for catabolism of small carbon sources

 

Aligments for a candidate for aldA in Paraburkholderia bryophila 376MFSha3.1

Align lactaldehyde dehydrogenase (EC 1.2.1.22); D-glyceraldehyde dehydrogenase (NADP+) (EC 1.2.1.89) (characterized)
to candidate H281DRAFT_03540 H281DRAFT_03540 succinate semialdehyde dehydrogenase (EC 1.2.1.16)

Query= BRENDA::P25553
         (479 letters)



>lcl|FitnessBrowser__Burk376:H281DRAFT_03540 H281DRAFT_03540
           succinate semialdehyde dehydrogenase (EC 1.2.1.16)
          Length = 479

 Score =  327 bits (839), Expect = 4e-94
 Identities = 186/468 (39%), Positives = 274/468 (58%), Gaps = 5/468 (1%)

Query: 10  YIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAEDARKAIDAAERAQPEWEALPAIERA 69
           YI G++  + G +   V+NPAT  VI+++  G A +A +AI AAERA P W +L A ER+
Sbjct: 10  YIGGEW--YEGASTYPVLNPATGEVIAQVAKGGAVEATQAIAAAERAFPAWRSLTAKERS 67

Query: 70  SWLRKISAGIRERASEISALIVEEGGKIQQLAEVEVAFTADYIDYMAEWARRYEGEIIQS 129
           + +++    + E    ++AL+  E GK    A  EV + A + ++ AE A+R  G++I S
Sbjct: 68  ARVKRWGELMLEHRDALAALLTREQGKPLAEARGEVGYAASFFEWFAEEAKRAYGDVIPS 127

Query: 130 DRPGENILLFKRALGVTTGILPWNFPFFLIARKMAPALLTGNTIVIKPSEFTPNNAIAFA 189
             P   I++ +  +GV   I PWNFP  +I RK  PAL  G T+V+KPSE TP +A+A A
Sbjct: 128 PNPNAKIIVTREPVGVVAAITPWNFPLAMITRKAGPALAAGCTMVLKPSEETPLSALALA 187

Query: 190 KIVDEIGLPRGVFNLVLGRGETVGQELAGNPKVAMVSMTGSVSAGEKIMATAAKNITKVC 249
            + ++ G+P GVFN+V G    +G  L  +  V  +S TGS   G+ +   +A  + K+ 
Sbjct: 188 VLAEKAGIPPGVFNVVSGDAVAIGGALTESDVVRKLSFTGSTRVGKLLAKQSADTLKKLS 247

Query: 250 LELGGKAPAIVMDDADLELAVKAIVDSRVINSGQVCNCAERVYVQKGIYDQFVNRLGEAM 309
           LELGG AP IV DDADL+ AV+  + S+  N+GQ C C  R YVQ GIYD F   L +A 
Sbjct: 248 LELGGNAPFIVFDDADLDAAVQGAMASKFRNTGQTCVCVNRFYVQDGIYDAFTLALAQAA 307

Query: 310 QAVQFGNPAERNDIAMGPLINAAALERVEQKVARAVEEGARVAFGGKAVEGKGYYYPPTL 369
           + ++ GN A + D+  GPLIN AAL +VE  VA A+++GA+V  G K     G +Y PT+
Sbjct: 308 RKMRVGN-ALQGDVEQGPLINQAALTKVEAHVADALQKGAKVLTGAKPHALGGTFYEPTV 366

Query: 370 LLDVRQEMSIMHEETFGPVLPVVAFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKG 429
           L+D    M I  EETFGPV     F T ++A++ AN + +GL++  YT++L  A +  + 
Sbjct: 367 LVDASSSMLIAQEETFGPVAACFRFKTEDEAVAAANATPFGLSAYFYTRDLARAWRVGEA 426

Query: 430 LKFGETYINRENFEAMQGFHAGWRKSGIGGADGKHGLHEYLQTQVVYL 477
           L+ G   IN            G ++SG+G    K+GL EY  T++ Y+
Sbjct: 427 LESGMVGINEGILSTEVAPFGGVKQSGLGREGSKYGLDEY--TELKYM 472


Lambda     K      H
   0.318    0.135    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 530
Number of extensions: 17
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 1
Length of query: 479
Length of database: 479
Length adjustment: 34
Effective length of query: 445
Effective length of database: 445
Effective search space:   198025
Effective search space used:   198025
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory