Aligments for a candidate for braC in Paraburkholderia bryophila 376MFSha3.1

GapMind for catabolism of small carbon sources

Aligments for a candidate for braC in Paraburkholderia bryophila 376MFSha3.1

Align BraC, component of General L- (and D-)amino acid uptake porter (transports acidic, basic, polar, semipolar and hydrophobic amino acids). The amino and carboxyl groups do not need to be α since γ-aminobutyric acid (GABA) is a substrate. The system may function with additional binding proteins since L-alanine uptake is not dependent on BraC (characterized)
to candidate H281DRAFT_02405 H281DRAFT_02405 amino acid/amide ABC transporter substrate-binding protein, HAAT family

Query= TCDB::Q9L3M3
         (381 letters)



>lcl|FitnessBrowser__Burk376:H281DRAFT_02405 H281DRAFT_02405 amino
           acid/amide ABC transporter substrate-binding protein,
           HAAT family
          Length = 382

 Score =  206 bits (525), Expect = 7e-58
 Identities = 133/366 (36%), Positives = 190/366 (51%), Gaps = 12/366 (3%)

Query: 2   KKSLLSAVALTAMLAFSGNAWA----DVLIAVAGPLTGPNAAFGAQLQKGAEQAAADINA 57
           K + L+  AL A ++ +G A A    DV I  AGP+TG  A +G   Q G   A  DINA
Sbjct: 4   KMTQLAGAALVAAMSLAGTANAQSTDDVKIGFAGPMTGAQAHYGKDFQNGITLAVEDINA 63

Query: 58  AGG-INGEQIKIEL--GDDVSDPKQGISVANKFAADGVKFVIGHFNSGVSIPASEVYAEN 114
               I G+ ++  L   DD +DP+ G +VA K   DG+K ++GHFNSG +IPAS +YA  
Sbjct: 64  TKPVIGGKPVRFVLDSADDQADPRTGTTVAQKLVDDGIKGMLGHFNSGTTIPASRIYANA 123

Query: 115 GILRNHPGRDEPDLHGTGLWNTFRTCGRDDQQGAIAGKYLADHFKDAKIAVVHDKTPYGQ 174
           GI         P+    G   TFR    D QQG++AG +        KI +V D+T YGQ
Sbjct: 124 GIPEIAMAT-APEYTQQGFKTTFRMMTSDTQQGSVAGTFAVKTLGVKKIVIVDDRTAYGQ 182

Query: 175 GLADETKKAMNAAGVTEVIYEGINVGDKDFSALIAKMKEAGVSIIYWGGLHTEAGLIIRQ 234
           GLAD+ +KA  AAG T V  E  N    DF +++ K+K A   +IY+GG  ++A  +++Q
Sbjct: 183 GLADQFEKAAKAAGGTIVDREYTNDKAVDFKSILTKLKAANPDLIYYGGADSQAAPMVKQ 242

Query: 235 AADQGLKATLVSGDGIVSNELASIAGDAVAGTLNTFGPDPTAN-PANKELVEKFKAAGFN 293
               G+KA L+ G+ + +     IAGDA  GT+ +    P    P  K+ V K+K   FN
Sbjct: 243 MKALGIKAPLMGGEMVHTPTFIQIAGDAANGTVASLAGLPLEEMPGGKDYVAKYKKR-FN 301

Query: 294 P--EAYTLYSYAAMQTIAGAAKAAGSLDPEAVAKAMKEKGPFPTVLGDISFDEKGDPKIP 351
              + Y+ Y+Y     +  A K A S DP      + +         ++S+D KGD K  
Sbjct: 302 EDVQTYSPYAYDGAMAMFAAMKKANSTDPAKYLPVLAKTSMPAVTSSNLSYDAKGDLKNG 361

Query: 352 GYIMYE 357
           G  +Y+
Sbjct: 362 GITLYK 367


Lambda     K      H
   0.314    0.132    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 384
Number of extensions: 26
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 381
Length of database: 382
Length adjustment: 30
Effective length of query: 351
Effective length of database: 352
Effective search space:   123552
Effective search space used:   123552
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources