GapMind for catabolism of small carbon sources

 

Aligments for a candidate for davD in Paraburkholderia bryophila 376MFSha3.1

Align Glutarate-semialdehyde dehydrogenase; EC 1.2.1.- (characterized)
to candidate H281DRAFT_02680 H281DRAFT_02680 succinate semialdehyde dehydrogenase

Query= SwissProt::Q9I6M5
         (483 letters)



>lcl|FitnessBrowser__Burk376:H281DRAFT_02680 H281DRAFT_02680
           succinate semialdehyde dehydrogenase
          Length = 492

 Score =  669 bits (1726), Expect = 0.0
 Identities = 328/482 (68%), Positives = 385/482 (79%)

Query: 2   QLKDAKLFRQQAYVDGAWVDADNGQTIKVNNPATGEIIGSVPKMGAAETRRAIEAADKAL 61
           +L D  L R  AY+DG W  AD+ +T  V++PATGE I  VP M  AETRRAIEA + A 
Sbjct: 11  RLADPSLLRTLAYIDGQWCGADDARTFAVDDPATGEKIADVPLMTGAETRRAIEAGEHAQ 70

Query: 62  PAWRALTAKERANKLRRWFDLMIENQDDLARLMTIEQGKPLAEAKGEIAYAASFLEWFGE 121
             WR LTA +R+  L+RW  LMI N DDLA +M+ EQGKPLAEAKGEI YAASF+EWF E
Sbjct: 71  RGWRKLTAAQRSTILKRWHALMIANTDDLAIIMSAEQGKPLAEAKGEIGYAASFIEWFAE 130

Query: 122 EAKRIYGDTIPGHQPDKRIIVIKQPIGVTAAITPWNFPSAMITRKAGPALAAGCTMVLKP 181
           +AKR+ GD +     DKR++V K+PIGV AAITPWNFP+AMITRK  PALAAGC M+LKP
Sbjct: 131 QAKRVDGDVLASPAADKRMLVTKEPIGVCAAITPWNFPAAMITRKVAPALAAGCAMILKP 190

Query: 182 ASQTPYSALALAELAERAGIPKGVFSVVTGSAGEVGGELTSNPIVRKLTFTGSTEIGRQL 241
           A  TP SALALAELA RAG+P GVFSVV G    +G E+TSNPIVRKL+FTGST +GR L
Sbjct: 191 AEATPLSALALAELAHRAGVPAGVFSVVVGDPRSIGAEMTSNPIVRKLSFTGSTPVGRML 250

Query: 242 MAECAQDIKKVSLELGGNAPFIVFDDADLDAAVEGALISKYRNNGQTCVCANRLYVQDGV 301
           M++CA  +KK+SLELGGNAPFIVFDDADLDAAVEGAL SKYRN GQTCVC NR+YVQDGV
Sbjct: 251 MSQCAPTVKKLSLELGGNAPFIVFDDADLDAAVEGALASKYRNAGQTCVCTNRVYVQDGV 310

Query: 302 YDAFVDKLKAAVAKLNIGNGLEAGVTTGPLIDAKAVAKVEEHIADAVSKGAKVVSGGKPH 361
           YDAF +K  AAV ++ +GNG E+GVT GPLI+  AV KVE HIADAV+ GA+V++GGK H
Sbjct: 311 YDAFAEKFAAAVGRIKVGNGFESGVTQGPLINEAAVEKVEAHIADAVAHGARVLTGGKRH 370

Query: 362 ALGGTFFEPTILVDVPKNALVSKDETFGPLAPVFRFKDEAEVIAMSNDTEFGLASYFYAR 421
           A G  FFEPT++ DV      + +ETFGP+AP+FRF +E E IA +N TEFGLA+YFY+R
Sbjct: 371 AAGKLFFEPTVVGDVTARMRFATEETFGPVAPLFRFTNEREAIAAANATEFGLAAYFYSR 430

Query: 422 DLARVFRVAEQLEYGMVGINTGLISNEVAPFGGIKASGLGREGSKYGIEDYLEIKYLCLG 481
           D+ R++RVAE LEYGMVGINTGLISNEVAPFGG+K SGLGREGSKYGIEDYLEIKYLC+G
Sbjct: 431 DIGRIWRVAEALEYGMVGINTGLISNEVAPFGGVKQSGLGREGSKYGIEDYLEIKYLCMG 490

Query: 482 GI 483
           G+
Sbjct: 491 GL 492


Lambda     K      H
   0.317    0.135    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 711
Number of extensions: 13
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 483
Length of database: 492
Length adjustment: 34
Effective length of query: 449
Effective length of database: 458
Effective search space:   205642
Effective search space used:   205642
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory