GapMind for catabolism of small carbon sources

 

Alignments for a candidate for davT in Paraburkholderia bryophila 376MFSha3.1

Align 4-aminobutyrate aminotransferase PuuE; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase; EC 2.6.1.19 (characterized)
to candidate H281DRAFT_03179 H281DRAFT_03179 4-aminobutyrate aminotransferase apoenzyme

Query= SwissProt::P50457
         (421 letters)



>FitnessBrowser__Burk376:H281DRAFT_03179
          Length = 429

 Score =  583 bits (1503), Expect = e-171
 Identities = 291/421 (69%), Positives = 337/421 (80%)

Query: 1   MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYIDFAAGIAVLNTGHRHPD 60
           + N E   R+ +ATPRGVGVMC+F+A+ A+NA L DVEG  +IDFAAGIAV NTGHRHP 
Sbjct: 3   LKNAELKSRKDAATPRGVGVMCDFYAERAQNAELWDVEGRRFIDFAAGIAVCNTGHRHPK 62

Query: 61  LVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQAKTAFFTTGAEAVENAVKI 120
           +VAA+  QL +FTHTAYQIVPY SYV LAEK+N  AP     KTAFFTTGAEAVENA+KI
Sbjct: 63  IVAAIRDQLDRFTHTAYQIVPYASYVELAEKLNERAPGDHPKKTAFFTTGAEAVENAIKI 122

Query: 121 ARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGPFPGSVYHVPYPSDLHGISTQ 180
           ARA TGRPGVIAF+GGFHGRT M MALTGKVAPYK GFGPFP  V+H P+P+ LHG+ST 
Sbjct: 123 ARAATGRPGVIAFTGGFHGRTLMGMALTGKVAPYKAGFGPFPSDVFHAPFPNPLHGVSTA 182

Query: 181 DSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPKELVAAIRRLCDEHGIVMIADEV 240
           DSL AIE LFK+DI+ K+VAAIIFEPVQGEGGF  AP E V A+R+LC+EHGI++IADEV
Sbjct: 183 DSLKAIEFLFKADIDPKRVAAIIFEPVQGEGGFYPAPAEFVRALRKLCNEHGILLIADEV 242

Query: 241 QSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLSGVVGNANIMDAPAPGGLGGTYAGN 300
           Q+GFARTGKLFAM HY   PDLMT+AKSLAGGMPLSGV+G A++MDA APGGLGGTYAGN
Sbjct: 243 QTGFARTGKLFAMHHYDVVPDLMTVAKSLAGGMPLSGVIGRADVMDAAAPGGLGGTYAGN 302

Query: 301 PLAVAAAHAVLNIIDKESLCERANQLGQRLKNTLIDAKESVPAIAAVRGLGSMIAVEFND 360
           PLAVAAA AVL+IID+E LCERA  LG R+K  LI  +   P IA VRG G M+AVEF  
Sbjct: 303 PLAVAAALAVLDIIDEEKLCERATILGDRVKAKLIALQNEAPQIADVRGPGGMVAVEFCK 362

Query: 361 PQTGEPSAAIAQKIQQRALAQGLLLLTCGAYGNVIRFLYPLTIPDAQFDAAMKILQDALS 420
           P + EP A   +++Q RAL +GLLLL CG Y NV+RFL+PLTI D  FD A+ IL+D + 
Sbjct: 363 PGSTEPDAEFTKRVQTRALKRGLLLLVCGVYSNVVRFLFPLTIEDTVFDEALAILEDVIK 422

Query: 421 D 421
           D
Sbjct: 423 D 423


Lambda     K      H
   0.319    0.134    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 664
Number of extensions: 25
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 421
Length of database: 429
Length adjustment: 32
Effective length of query: 389
Effective length of database: 397
Effective search space:   154433
Effective search space used:   154433
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory