GapMind for catabolism of small carbon sources

 

Aligments for a candidate for kauB in Paraburkholderia bryophila 376MFSha3.1

Align 4-guanidinobutyraldehyde dehydrogenase (EC 1.2.1.54) (characterized)
to candidate H281DRAFT_02464 H281DRAFT_02464 betaine aldehyde dehydrogenase

Query= metacyc::MONOMER-11560
         (497 letters)



>lcl|FitnessBrowser__Burk376:H281DRAFT_02464 H281DRAFT_02464 betaine
           aldehyde dehydrogenase
          Length = 489

 Score =  392 bits (1006), Expect = e-113
 Identities = 205/479 (42%), Positives = 297/479 (62%), Gaps = 6/479 (1%)

Query: 21  RAFINGEYTDAVSGETFECLSPVDGRFLAKVASCDLADANRAVENARATFNSGVWSQLAP 80
           R +I G Y DA  GETF+ + P +G  LA V     AD +RAV++AR       W+ L  
Sbjct: 8   RLYIGGTYVDATGGETFDTVDPANGETLATVQQASSADVDRAVQSARE--GQREWAALTG 65

Query: 81  AKRKAKLIRFADLLRKNVEELALLETLDMGKPIGDSSSIDIPGAAQAIHWTAEAIDKVYD 140
            +R   L R  D+LR+  +ELA LET D GKPI ++ ++DI   A  I + A     +  
Sbjct: 66  MQRSRILRRAVDILRERNDELAALETRDTGKPIAETQAVDIVTGADVIEYYAGLATAIEG 125

Query: 141 EVAPTPHDQLGLVTREPVGVVGAIVPWNFPLLMACWKLGPALATGNSVVLKPSEKSPLTA 200
           +  P          REP+GV   I  WN+P+ +ACWK  PALA GN+++ KPSE +PL+A
Sbjct: 126 QQIPLRPTSFVYTRREPLGVCAGIGAWNYPIQIACWKSAPALAAGNAMIFKPSEVTPLSA 185

Query: 201 IRIAQLAIEAGIPAGVLNVLPGYGHTVGKALALHMDVDTLVFTGSTKIAKQLMVYAGESN 260
           +++A++  EAG+P GV NV+ G G  VG  LA H D++ + FTG  +  K++M  AG S+
Sbjct: 186 LKLAEIYTEAGVPPGVFNVVQGDGR-VGAMLAAHPDIEKISFTGGVETGKKVMSMAGASS 244

Query: 261 MKRIWLEAGGKSPNIVFADAPDLQAAAEAAASAIAFNQGEVCTAGSRLLVERSIKDKFLP 320
           +K + +E GGKSP +VF DA +L+ AA+ A SA  F+ G+VCT G+R+ V+RS+ ++F  
Sbjct: 245 LKEVTMELGGKSPLLVFDDA-NLERAADIAMSANFFSSGQVCTNGTRVFVQRSVLERFEA 303

Query: 321 MVVEALKGWKPGNPLDPQTTVGALVDTQQMNTVLSYIEAGHKDGAKLLAGGKRTLEE--T 378
           +V+E +K  + G P D  T  G LV   Q+  VL YI++G ++GA+L+AGGKR  E    
Sbjct: 304 LVLERVKRIRVGAPGDASTNFGPLVSAAQLQKVLGYIDSGVQEGARLIAGGKRLSEGHFG 363

Query: 379 GGTYVEPTIFDGVTNAMRIAQEEIFGPVLSVIAFDTAEEAVAIANDTPYGLAAGIWTSDI 438
            G YVEPT+F G  + MRI +EEIFGPV+S++ FD  +EA+  AN T YGLAAG+ T ++
Sbjct: 364 QGQYVEPTVFTGCHDDMRIVREEIFGPVMSILIFDNEDEAIERANRTAYGLAAGVVTENL 423

Query: 439 SKAHKTARAVRAGSVWVNQYDGGDMTAPFGGFKQSGNGRDKSLHALEKYTELKATWIKL 497
           ++AH+    + AG  W+N +       P GG+KQSG GR+  +  LE YT +K+  ++L
Sbjct: 424 ARAHRVIHRLEAGICWINTWGESPAEMPVGGYKQSGVGRENGITTLEHYTRIKSVQVEL 482


Lambda     K      H
   0.316    0.132    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 650
Number of extensions: 32
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 497
Length of database: 489
Length adjustment: 34
Effective length of query: 463
Effective length of database: 455
Effective search space:   210665
Effective search space used:   210665
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory