Align TM0027, component of β-glucoside porter (Conners et al., 2005). Binds cellobiose, laminaribiose (Nanavati et al. 2006). Regulated by cellobiose-responsive repressor BglR (characterized)
to candidate H281DRAFT_01031 H281DRAFT_01031 peptide/nickel transport system ATP-binding protein
Query= TCDB::Q9WXN4 (268 letters) >FitnessBrowser__Burk376:H281DRAFT_01031 Length = 377 Score = 171 bits (433), Expect = 2e-47 Identities = 97/239 (40%), Positives = 140/239 (58%), Gaps = 8/239 (3%) Query: 23 IEAVKNVSFEVKEKEIVSLVGESGSGKTTTAKMILRLLPPTSGEIYFEGKDI--WKDIKD 80 + AV VSF V E ++VGESG GK+T +M+LRL+ T G + ++G+DI W+ K Sbjct: 40 VHAVNEVSFAVHTGETFAIVGESGCGKSTLGRMLLRLIDATQGRVLYQGEDITHWQGAKL 99 Query: 81 RESLVEFRRKVHAVFQDPFASYNPFYPVERTLWQAISLLENKPSNKKEALELIKESLFRV 140 R RR + +FQDPFAS NP V + + + ++ S+ E E + + L +V Sbjct: 100 RR----LRRDMQIIFQDPFASLNPGMTVGQIIGEPVAF-HGLASSGSECRERVAQLLTKV 154 Query: 141 GIDPKDVLGKYPHQISGGQKQRIMIARCWILRPLLIVADEPTSMIDASSRGGIIKLLEEL 200 G+ P +YPH+ SGGQ+QRI IAR P LIV DEP S +D S + +I LLE L Sbjct: 155 GLQPA-YAQRYPHEFSGGQRQRIGIARALAGEPKLIVGDEPVSALDVSVQAQVINLLESL 213 Query: 201 REEQGTSIIFITHDLGLAYYVSDNIFVMKNGEIVERGHPDKVVLEPTHEYTKLLVGSIP 259 + E G ++I + HDL + ++SD + VM GEIVE D++ P H YT+ L+ +IP Sbjct: 214 KAELGLTLIMVAHDLAVIRHMSDRVAVMYLGEIVELAQVDELFDAPLHPYTQALLRAIP 272 Lambda K H 0.319 0.139 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 249 Number of extensions: 7 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 268 Length of database: 377 Length adjustment: 27 Effective length of query: 241 Effective length of database: 350 Effective search space: 84350 Effective search space used: 84350 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 48 (23.1 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory