Align Beta-glucosidase (EC 3.2.1.21) (characterized)
to candidate H281DRAFT_00016 H281DRAFT_00016 beta-glucosidase
Query= reanno::Caulo:CCNA_01019 (758 letters) >FitnessBrowser__Burk376:H281DRAFT_00016 Length = 763 Score = 456 bits (1172), Expect = e-132 Identities = 291/762 (38%), Positives = 415/762 (54%), Gaps = 42/762 (5%) Query: 3 STTLRSLSLALLISASASALSVVPALA-DAAKPAAALAHPWMDKSLDADKRADLVLKAMT 61 S LR + + A SA+SV ++ A P AA PWM+ +L ++RA L++ AM Sbjct: 7 SPHLRGIPHRAIRIACESAVSVAALVSLSAGAPQAASGPPWMNTALAPEQRAALLVGAMA 66 Query: 62 NDEKFTLIFGYFGADMKPKYTRIPESLPGSAGYIAGVPRLGIPAQFQTDAGVGVATQGSA 121 D+K + G G +P + S +I G+P L IP T+ VG+ Sbjct: 67 LDQKAAQLHGQSGT-----IAEVP-ACGNSIRHIPGIPALSIPTFRITNGPVGIGAGDCV 120 Query: 122 KEFRERTALPSGMATTATWNPELAFQGGAMIGKEARDSGFNVQLAGGVNLVREPRNGRNF 181 + R TALP MA A+++P LA G ++G+EAR G +V A G+N VR + GRNF Sbjct: 121 PQDRA-TALPMSMALAASFDPTLARAYGDVLGREARMLGLHVVEAPGMNAVRVGQGGRNF 179 Query: 182 EYGGEDPLLAGVMVAAQIRGIQSNNIISTIKHYALNAQETGRFVVSSNIDDTAARMSDLL 241 EY EDP+LAGV+ +IRG Q+ +I+ KH+ LN QE R VS +DD LL Sbjct: 180 EYLSEDPVLAGVIAENEIRGAQATGVIAMAKHFILNEQEANRNTVSVVVDDRTLHEIYLL 239 Query: 242 AFQFAIEQSDPHSVMCAYNRVNSVYACESDWLLNTVLKKDWGYKGYVMSDWGAAHSSAKA 301 F ++ + + SVMC+YNR+ S YACE+ LN +L+ + G+ GYV SD+GA HS+A A Sbjct: 240 PFGVSVRRGNVASVMCSYNRIGSTYACETPRALNAILRGELGFMGYVQSDFGATHSTAPA 299 Query: 302 ANAGLDQESAGDAFDKQPFFAAPLKADLASGAVSQARIDDMARRVLRAMFASGVVDHPLT 361 NAGLD E + F L A LA+G ++ A ++ R MF +G+ D P+ Sbjct: 300 LNAGLDLEMQSGIW----FTPPNLNAALATGQLTMATVNQALTRRYTQMFKAGIFDRPVA 355 Query: 362 GAPLAMPAETLAAHAKITQADAEEGIVLLKNDGGLLPLAKTAKTIAVIG--SYADVGVLS 419 PL + T+ + E+ VLLKN G LLPL+ K+I ++G +Y D + Sbjct: 356 RTPL--DSTTIQNDGTEARVIGEQSAVLLKNSGNLLPLSGAVKSITIVGHNTYVDDAIAG 413 Query: 420 GGGSSQVYPVGGRAVQGLEPKTWPGPVIYY--PSSPLKAIAARYPGAKVVYDDGTDPARA 477 G GSS+V P + P+ V+ S+ +K +AA +DG+ A Sbjct: 414 GSGSSRVVP-----TYTVGPQQGITNVLRQLGSSATVKTLAAP--------NDGSMDAAV 460 Query: 478 AKLAASSDLALVFADQWTTESVDVTTLNLPKNQDATIDAVASANKKTVVVLLTGGPLLMP 537 A AA++D+A+V A T E D L+LPK QD I AVA+ N++T+VVL G P+LMP Sbjct: 461 AAAAATADVAIVMAGVVTGEGSDRPDLSLPKGQDKLIAAVANRNERTIVVLKDGDPVLMP 520 Query: 538 WLDKVGAVVEAWFPGTAGGEAIARVLTGEVDASGRLPVTFPKSVAELPRPKLDGLGKPDG 597 W+D+V A++E W+ G G +AR+L G + SG+LPVT+P+ + P D G Sbjct: 521 WVDQVPAILETWYSGQEDGNVVARLLFGLANPSGKLPVTYPRLATDTPTSMPDRYPGTSG 580 Query: 598 EMFDVDYTLEGAAVGYKWYDLKKIEPLFAFGHGLSYTQFAYSNLTASASGDT----LTVS 653 V Y+ EG VGY+WYD ++++PLFAFGHGLSYT F S++ S G T + V+ Sbjct: 581 SDPVVQYS-EGLLVGYRWYDTRQVKPLFAFGHGLSYTSFKISDVQVSPRGYTGNGPILVA 639 Query: 654 FEVKNVGRRPGKDVPQVYVGPKAGGWEAPKRLAGFQKVSLAPGASQRVTVTVDP----RL 709 V+N G R G +V QVY+G E KRL FQKV L PG + +++ + P Sbjct: 640 LTVENTGLREGAEVAQVYLGLPPNLGEPTKRLIAFQKVLLKPGEKRALSMYIYPINGFHA 699 Query: 710 LAVWDSKAHGWSIAAGQYDIALGASSRAI--SSTAQVTLAAR 749 L+ WD+ A W IAAG Y + +G +S I ST V +A R Sbjct: 700 LSYWDNAAQSWKIAAGTYTVYVGNASDNIVAQSTFTVNVAMR 741 Lambda K H 0.316 0.131 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1220 Number of extensions: 54 Number of successful extensions: 9 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 758 Length of database: 763 Length adjustment: 40 Effective length of query: 718 Effective length of database: 723 Effective search space: 519114 Effective search space used: 519114 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 55 (25.8 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory