Alignments for a candidate for astC in Paraburkholderia bryophila 376MFSha3.1

GapMind for catabolism of small carbon sources

Alignments for a candidate for astC in Paraburkholderia bryophila 376MFSha3.1

Align succinylornithine transaminase (EC 2.6.1.81) (characterized)
to candidate H281DRAFT_06478 H281DRAFT_06478 succinylornithine aminotransferase apoenzyme

Query= BRENDA::O30508
         (406 letters)



>FitnessBrowser__Burk376:H281DRAFT_06478
          Length = 411

 Score =  506 bits (1303), Expect = e-148
 Identities = 239/397 (60%), Positives = 308/397 (77%)

Query: 8   VERADFDRYMVPNYAPAAFIPVRGEGSRVWDQSGRELIDFAGGIAVTSLGHAHPALVKAL 67
           V R  FD  MVP +APAAF+P RG GSRVWD  GR+ IDFAGGIAVT+LGHAHP L+K L
Sbjct: 6   VTRKTFDEVMVPVFAPAAFVPDRGLGSRVWDTQGRDYIDFAGGIAVTALGHAHPELLKVL 65

Query: 68  TEQAQRIWHVSNVFTNEPALRLARKLVDATFAERVFLANSGAEANEAAFKLARRYANDVY 127
            EQ  ++WH+ N +TNEP LRLAR+L + TFA+R F ANSGAEANEAA KLARR A + +
Sbjct: 66  HEQGSKLWHIGNGYTNEPVLRLARRLEELTFADRAFFANSGAEANEAALKLARRVAFERH 125

Query: 128 GPQKYEIIAASNSFHGRTLFTVNVGGQPKYSDGFGPKFEGITHVPYNDLEALKAAISDKT 187
           G  KYEII+ + SFHGRT FTV+VGGQPKYS+GFGP  +GI H+PYND++A + AI  KT
Sbjct: 126 GADKYEIISFTQSFHGRTFFTVSVGGQPKYSEGFGPVPQGIVHLPYNDIQAAQKAIGAKT 185

Query: 188 CAVVLEPIQGEGGVLPAQQAYLEGARKLCDEHNALLVFDEVQSGMGRVGELFAYMHYGVV 247
           CAV++EPIQGEGGV+PA  A+L+  R+ CD+H ALL+FDEVQ+G+GR G  +AY   GV 
Sbjct: 186 CAVIVEPIQGEGGVIPADPAFLKALREACDQHGALLIFDEVQTGVGRSGYFYAYQDTGVT 245

Query: 248 PDILSSAKSLGGGFPIGAMLTTGEIAKHLSVGTHGTTYGGNPLASAVAEAALDVINTPEV 307
           PDIL++AK+LG GFPIGAMLTT E+A H  VG HGTTYGGNPL SA+AE  +++I+ P++
Sbjct: 246 PDILTTAKALGNGFPIGAMLTTNELAAHFKVGVHGTTYGGNPLGSAIAEKVVELISDPKL 305

Query: 308 LDGVKAKHERFKSRLQKIGQEYGIFDEIRGMGLLIGAALTDEWKGKARDVLNAAEKEAVM 367
           L+GV+ + E  K  L K+ + +G+FDE+RG GLLIGA LTD +KG+A+D + AA +  V+
Sbjct: 306 LEGVRTRSEVLKGHLAKLNERFGLFDEVRGKGLLIGAQLTDAYKGRAKDFVTAAGQHGVI 365

Query: 368 VLQASPDVVRFAPSLVIDDAEIDEGLERFERAVAKLV 404
           +L A PDV+RF PSL++   +++EG ER  +A+A +V
Sbjct: 366 MLMAGPDVLRFVPSLIMPLDDMNEGFERLAKAIADVV 402


Lambda     K      H
   0.318    0.135    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 554
Number of extensions: 16
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 406
Length of database: 411
Length adjustment: 31
Effective length of query: 375
Effective length of database: 380
Effective search space:   142500
Effective search space used:   142500
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

Align candidate H281DRAFT_06478 H281DRAFT_06478 (succinylornithine aminotransferase apoenzyme)
to HMM TIGR03246 (astC: succinylornithine transaminase family (EC 2.6.1.81))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR03246.hmm
# target sequence database:        /tmp/gapView.20787.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR03246  [M=397]
Accession:   TIGR03246
Description: arg_catab_astC: succinylornithine transaminase family
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                    Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                    -----------
   1.5e-215  701.5   0.1   1.8e-215  701.3   0.1    1.0  1  lcl|FitnessBrowser__Burk376:H281DRAFT_06478  H281DRAFT_06478 succinylornithin


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Burk376:H281DRAFT_06478  H281DRAFT_06478 succinylornithine aminotransferase apoenzyme
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  701.3   0.1  1.8e-215  1.8e-215       1     397 []       6     402 ..       6     402 .. 1.00

  Alignments for each domain:
  == domain 1  score: 701.3 bits;  conditional E-value: 1.8e-215
                                    TIGR03246   1 veresfdevmvpvyapakfipvrgeGsrvwdqegkeyidfaGGiavnalGhahpelvealkeqaek 66 
                                                  v+r++fdevmvpv+apa+f+p+rg Gsrvwd++g++yidfaGGiav+alGhahpel ++l+eq++k
  lcl|FitnessBrowser__Burk376:H281DRAFT_06478   6 VTRKTFDEVMVPVFAPAAFVPDRGLGSRVWDTQGRDYIDFAGGIAVTALGHAHPELLKVLHEQGSK 71 
                                                  79**************************************************************** PP

                                    TIGR03246  67 lwhlgngytnepvlrlakklvdatfadkvffcnsGaeaneaalklarkvaldkygaekseivafkn 132
                                                  lwh+gngytnepvlrla++l + tfad++ff+nsGaeaneaalklar+va++++ga+k+ei++f++
  lcl|FitnessBrowser__Burk376:H281DRAFT_06478  72 LWHIGNGYTNEPVLRLARRLEELTFADRAFFANSGAEANEAALKLARRVAFERHGADKYEIISFTQ 137
                                                  ****************************************************************** PP

                                    TIGR03246 133 sfhGrtlftvsvGGqakysedfaplpegikhaayndlealkalisdktcavivepiqGegGvvpad 198
                                                  sfhGrt+ftvsvGGq+kyse+f+p+p+gi h++ynd++a++++i  ktcavivepiqGegGv+pad
  lcl|FitnessBrowser__Burk376:H281DRAFT_06478 138 SFHGRTFFTVSVGGQPKYSEGFGPVPQGIVHLPYNDIQAAQKAIGAKTCAVIVEPIQGEGGVIPAD 203
                                                  ****************************************************************** PP

                                    TIGR03246 199 kaflkglrelcdrhnallifdevqtGvGrtGelyaymeyGvtpdiltsakalGgGfpiGallttee 264
                                                  +aflk+lre+cd+h+allifdevqtGvGr G++yay+++Gvtpdilt+akalG+GfpiGa+ltt+e
  lcl|FitnessBrowser__Burk376:H281DRAFT_06478 204 PAFLKALREACDQHGALLIFDEVQTGVGRSGYFYAYQDTGVTPDILTTAKALGNGFPIGAMLTTNE 269
                                                  ****************************************************************** PP

                                    TIGR03246 265 lakvlkvGthGttyGGnplacavaekvldlvntaelleGvkqrhelfvdelekinarykvfseirG 330
                                                  la+++kvG+hGttyGGnpl++a+aekv++l+++++lleGv++r+e++  +l+k+n+r+ +f+e+rG
  lcl|FitnessBrowser__Burk376:H281DRAFT_06478 270 LAAHFKVGVHGTTYGGNPLGSAIAEKVVELISDPKLLEGVRTRSEVLKGHLAKLNERFGLFDEVRG 335
                                                  ****************************************************************** PP

                                    TIGR03246 331 kGlliGavlteeyaGkakdlvnaaaeeGvlvliaGpdvvrfapslvieeeeikeGlarlekavekl 396
                                                  kGlliGa+lt++y+G+akd+v+aa ++Gv++l+aGpdv+rf psl+++ ++++eG++rl+ka++ +
  lcl|FitnessBrowser__Burk376:H281DRAFT_06478 336 KGLLIGAQLTDAYKGRAKDFVTAAGQHGVIMLMAGPDVLRFVPSLIMPLDDMNEGFERLAKAIADV 401
                                                  ***************************************************************988 PP

                                    TIGR03246 397 v 397
                                                  v
  lcl|FitnessBrowser__Burk376:H281DRAFT_06478 402 V 402
                                                  6 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (397 nodes)
Target sequences:                          1  (411 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 11.34
//
[ok]

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources