Align deoxyribose-phosphate aldolase (EC 4.1.2.4) (characterized)
to candidate H281DRAFT_01118 H281DRAFT_01118 deoxyribose-phosphate aldolase
Query= BRENDA::Q9Y315 (318 letters) >FitnessBrowser__Burk376:H281DRAFT_01118 Length = 337 Score = 370 bits (951), Expect = e-107 Identities = 187/314 (59%), Positives = 238/314 (75%), Gaps = 2/314 (0%) Query: 5 NRGTELDLSWISKIQVNHPAVLRRAEQIQARRTVKKEWQAAWLLKAVTFIDLTTLSGDDT 64 N G D SW+ +++N AV RR + RRTVKK+ QAAWLLKAVT IDLTTLSGDDT Sbjct: 26 NPGMPFDASWLDGLRINQSAVERRTATLATRRTVKKDAQAAWLLKAVTCIDLTTLSGDDT 85 Query: 65 SSNIQRLCYKAKYPIREDLLKALNMHDKGITTAAVCVYPARVCDAVKALKAAGCNIPVAS 124 ++RLC KA+ P+R DLL+AL M + +TT AVCVY V AV ALK +G IPVA+ Sbjct: 86 EGRVRRLCAKARQPVRADLLEALGMTPQRVTTGAVCVYHRFVAAAVDALKGSG--IPVAA 143 Query: 125 VAAGFPAGQTHLKTRLEEIRLAVEDGATEIDVVINRSLVLTGQWEALYDEIRQFRKACGE 184 V+ GFPAG +L+EI +V DGA EID+V+ R VLTG W+ALYDE+R+FR ACG Sbjct: 144 VSTGFPAGLIPHPLKLKEIEASVADGAAEIDIVVTREYVLTGNWQALYDEVREFRAACGP 203 Query: 185 AHLKTILATGELGTLTNVYKASMIAMMAGSDFIKTSTGKETVNATFPVAIVMLRAIRDFF 244 AHLK ILATG++ TL+N+ +ASM+ MMAG+DFIKTSTGKE VNAT V++VM+R IR++ Sbjct: 204 AHLKAILATGDIRTLSNIARASMVCMMAGADFIKTSTGKEGVNATLDVSLVMVRMIREYH 263 Query: 245 WKTGNKIGFKPAGGIRSAKDSLAWLSLVKEELGDEWLKPELFRIGASTLLSDIERQIYHH 304 +TG IGFKPAGG+ SAK L++ L+KEELG WL+PELFRIGAS+LL+DIERQ+ HH Sbjct: 264 ERTGVPIGFKPAGGVSSAKSVLSYQILMKEELGRAWLEPELFRIGASSLLADIERQLEHH 323 Query: 305 VTGRYAAYHDLPMS 318 V+GRY+A++ P++ Sbjct: 324 VSGRYSAFNRHPVA 337 Lambda K H 0.320 0.133 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 336 Number of extensions: 9 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 318 Length of database: 337 Length adjustment: 28 Effective length of query: 290 Effective length of database: 309 Effective search space: 89610 Effective search space used: 89610 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 48 (23.1 bits)
Align candidate H281DRAFT_01118 H281DRAFT_01118 (deoxyribose-phosphate aldolase)
to HMM TIGR00126 (deoC: deoxyribose-phosphate aldolase (EC 4.1.2.4))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR00126.hmm # target sequence database: /tmp/gapView.24267.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00126 [M=211] Accession: TIGR00126 Description: deoC: deoxyribose-phosphate aldolase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.2e-44 137.5 0.1 1.2e-43 135.0 0.1 1.8 1 lcl|FitnessBrowser__Burk376:H281DRAFT_01118 H281DRAFT_01118 deoxyribose-phos Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Burk376:H281DRAFT_01118 H281DRAFT_01118 deoxyribose-phosphate aldolase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 135.0 0.1 1.2e-43 1.2e-43 3 201 .. 72 295 .. 70 300 .. 0.91 Alignments for each domain: == domain 1 score: 135.0 bits; conditional E-value: 1.2e-43 TIGR00126 3 akliDhtalkadtteedietlcaeAkky................kfaavcvnpsyvslAkelLkgt 52 + iD+t+l+ d+te + +lca+A ++ + avcv+ ++v A++ Lkg+ lcl|FitnessBrowser__Burk376:H281DRAFT_01118 72 VTCIDLTTLSGDDTEGRVRRLCAKARQPvradllealgmtpqrvTTGAVCVYHRFVAAAVDALKGS 137 578**********************9987777777777777766688******************* PP TIGR00126 53 eveictv.vgFPlGasttevkllEakeaieeGAdEvDvviniaalkdkneevviedikavveacak 117 + +++v +gFP+G + kl E +++++ GA E+D+v+ + +n+++ +++++ ac+ lcl|FitnessBrowser__Burk376:H281DRAFT_01118 138 GIPVAAVsTGFPAGLIPHPLKLKEIEASVADGAAEIDIVVTREYVLTGNWQALYDEVREFRAACGP 203 ******978********************************************************* PP TIGR00126 118 vllKvilEtalLtd.eekkkAseisieagadfvKtstgfsakgAtvedvrlmkkvvgd.......e 175 ++lK+il t+ ++ +++ +As +++ agadf+Ktstg+ ++At++ +m +++++ lcl|FitnessBrowser__Burk376:H281DRAFT_01118 204 AHLKAILATGDIRTlSNIARASMVCMMAGADFIKTSTGKEGVNATLDVSLVMVRMIREyhertgvP 269 **********996527778***************************99999999999999999999 PP TIGR00126 176 vgvKasGGvrtaedalalieagaeri 201 +g+K++GGv a+ +l++ e + lcl|FitnessBrowser__Burk376:H281DRAFT_01118 270 IGFKPAGGVSSAKSVLSYQILMKEEL 295 *************9998865555555 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (211 nodes) Target sequences: 1 (337 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 3.77 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory