Alignments for a candidate for adhAqn in Paraburkholderia bryophila 376MFSha3.1

GapMind for catabolism of small carbon sources

Alignments for a candidate for adhAqn in Paraburkholderia bryophila 376MFSha3.1

Align alcohol dehydrogenase (quinone) (EC 1.1.5.5) (characterized)
to candidate H281DRAFT_01296 H281DRAFT_01296 PQQ-dependent dehydrogenase, methanol/ethanol family

Query= BRENDA::Q44002
         (739 letters)



>FitnessBrowser__Burk376:H281DRAFT_01296
          Length = 605

 Score =  284 bits (727), Expect = 8e-81
 Identities = 195/585 (33%), Positives = 280/585 (47%), Gaps = 58/585 (9%)

Query: 26  ISGYATMASADDGQGATGEAIIHADDH-------PGNWMTYGRTYSEQRYSPLDQINRSN 78
           + G A +ASA     A    +  AD         P NW      Y+  RYSPL QIN +N
Sbjct: 7   VLGLAILASA-----AMSSIVAQADSQLDGLMKSPSNWAAQAGDYANHRYSPLKQINENN 61

Query: 79  VGNLKLAWYLDLDTNRGQEGTPLVIDGVMYATTNW-SMMKAVDAATGKLLWSYDPRVPGN 137
           VG L++AW +     RG EG PLVI   MY  + + + + A++      LW Y P+   +
Sbjct: 62  VGKLQVAWTMSTGVLRGHEGAPLVIGDTMYIHSPFPNKVIAINLKDQTFLWQYQPKQDDS 121

Query: 138 IADKGCCDTVNRGAAYWNGKVYFGTFDGRLIALDAKTGKLVWSVNTIPPEAELGKQRS-Y 196
           +    CCDTVNRG AY +GK++    D +L+AL+AKTG +VW+       A+ G  ++  
Sbjct: 122 VVSVMCCDTVNRGLAYGDGKIFLQQADTKLVALNAKTGDVVWT-------AQNGNSKAGE 174

Query: 197 TVDGAPRIAKGRVIIGNGGSEFGARGFVTAFDAETGKVDWRFFTAPNPKN---EPDHTAS 253
           T   AP +   +V+ G  G EFG RG + A+D +TGK  W  ++    K+   +PD T +
Sbjct: 175 TNTNAPHVFGDKVLTGISGGEFGVRGRLIAYDIKTGKPAWTAYSTGPDKDMLMDPDKTMT 234

Query: 254 DS------VLMNKAYQTWSPTGAWTRQGGGGTVWDSIVYDPVADLVYLGVGNGSPWNYKY 307
            +      V  + + ++W     W  + GGGT W    +DP  +LVY G GN   WN   
Sbjct: 235 YADGKMVPVGADSSLKSWK-GDQW--KLGGGTTWGWYAWDPKLNLVYYGTGNPGTWNPTQ 291

Query: 308 RSEGKGDNLFLGSIVALKPETGEYVWHFQETPMDQWDFTSVQQIMTLDLPINGETRHVIV 367
           R    GDN +  SI+A    TG   W +Q TP D+WD+  V +++  DL I+G+    IV
Sbjct: 292 R---PGDNKWSMSIMARDLNTGMAKWVYQMTPHDEWDYDGVNEMILSDLTIDGKKVPAIV 348

Query: 368 HAPKNGFFYIIDAKTGEFISGKNY-VYVNWASGLDPKTGRPIYNPDALYTLTGKEW--YG 424
           H  +NGF Y ++ ++G+ +  + +   VNWA  +D K+G+PI N        G +    G
Sbjct: 349 HFDRNGFGYTLNRESGQLLVAQKFDPAVNWADSVDLKSGKPIRNAAYSTQAAGSDHNVKG 408

Query: 425 I-PGDLGGHNFAAMAFSPKTGLVYIPAQQVPFLY----TNQVGGFTPHPDSWNLGLDMNK 479
           I P  LG  +    A+ P + L  +P   V   Y     + V G  P+     +G  ++ 
Sbjct: 409 ICPAALGSKDQQPAAYDPNSSLFLVPTNHVCMDYEPFDVDYVSG-QPY-----VGATLSM 462

Query: 480 VGIPDSPEAKQAFVKDLKGWIVAWDPQKQAEAWRVDHKGPWNGGILATGGDLLFQGLANG 539
              P+   A         G  +AWD  K    W    K     G LAT G + F G   G
Sbjct: 463 YPGPNENNA--------MGNFIAWDAAKGKIVWSKPEKFSVWSGALATAGGIAFYGTLEG 514

Query: 540 EFHAYDATNGSDLFHFAADSGIIAPPVTYLANGKQYVAVEVGWGG 584
              A    +G +L+ F   SGII    TY   GKQYV V  G GG
Sbjct: 515 YLKAVRIKDGKELWRFKTPSGIIGNVFTYQYQGKQYVGVYSGIGG 559


Lambda     K      H
   0.318    0.137    0.440 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1749
Number of extensions: 144
Number of successful extensions: 14
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 739
Length of database: 605
Length adjustment: 38
Effective length of query: 701
Effective length of database: 567
Effective search space:   397467
Effective search space used:   397467
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources