Alignments for a candidate for gguA in Paraburkholderia bryophila 376MFSha3.1

GapMind for catabolism of small carbon sources

Alignments for a candidate for gguA in Paraburkholderia bryophila 376MFSha3.1

Align GguA aka ATU2347 aka AGR_C_4264, component of Multiple sugar (arabinose, xylose, galactose, glucose, fucose) putative porter (characterized)
to candidate H281DRAFT_02712 H281DRAFT_02712 monosaccharide ABC transporter ATP-binding protein, CUT2 family

Query= TCDB::O05176
         (512 letters)



>FitnessBrowser__Burk376:H281DRAFT_02712
          Length = 505

 Score =  388 bits (996), Expect = e-112
 Identities = 214/498 (42%), Positives = 323/498 (64%), Gaps = 9/498 (1%)

Query: 6   LEMRNITKTFPGVKALENVNLKVKEGEIHALVGENGAGKSTLMKVLSGVYPAGTYEGEIH 65
           LEMRNI++TFPGVKAL+ VNL+++ GE+ AL GENGAGKSTLMK+L+G+Y A    G I 
Sbjct: 10  LEMRNISRTFPGVKALDRVNLEIRAGEVLALAGENGAGKSTLMKILTGIY-APDPGGTIL 68

Query: 66  YEGAVRNFRAINDSEDIGIIIIHQELALVPLLSIAENIFLGNEVASN-GVISWQQTFNRT 124
            EG        + +  +G+ II+QELA+V  L++ ENIFL  E  +  G+I   + +   
Sbjct: 69  VEGQEVALADSHHARTLGVNIIYQELAVVGNLTVGENIFLAREPRTRLGLIDRPRMYREA 128

Query: 125 RELLKKVGLKESPETLITDIGVGKQQLVEIAKALSKSVKLLILDEPTASLNESDSEALLN 184
           RE+L  + +   P T ++++ VG+QQ++EIAKAL    K +I+DEPTASL+  ++  LL 
Sbjct: 129 REVLATIDMDIDPATRVSELSVGQQQMIEIAKALCARSKAIIMDEPTASLSHHETSVLLG 188

Query: 185 LLMEFRNQGMTSIIITHKLNEVRKVADQITVLRDGMTVKTLDCHQEEISEDVIIRNMVGR 244
           ++   R + +  + I+H+L E+ ++AD++TVLRDG TV T      +++ + ++R MV R
Sbjct: 189 IVKRLRERNIAVVYISHRLEEIFELADRVTVLRDGRTVGTAPI--ADMTRETLVRLMVAR 246

Query: 245 DLEDRY-PPRDVPIGETILEVKNWNAYHQQHRDRQVLHDINVTVRKGEVVGIAGLMGAGR 303
           +L + Y  P+     + +LEV+  +    +  + ++  DI+ T+ +GEV+GIAGL+G+GR
Sbjct: 247 ELSELYGEPQSHASRDPVLEVRALSLKPVRKAEPRI-RDISFTLHRGEVLGIAGLVGSGR 305

Query: 304 TEFAMSVFGKSYGHRITGDVLIDGKPVDVSTVRKAIDAGLAYVTEDRKHLGLVLNDNILH 363
           TE    +FG       TG V I+GKPV +     AI +G+ +VTEDRK  GL+L   +  
Sbjct: 306 TEIMEMIFGM---RACTGSVKIEGKPVSIRNPHDAIRSGIGFVTEDRKAQGLILGMTVRE 362

Query: 364 NTTLANLAGVSKASIIDDIKEMKVASDFRTRLRIRSSGIFQETVNLSGGNQQKVVLSKWL 423
           N +L +L   S    +   +E +    F   L I++ G+ Q+ VNLSGGNQQK+V++KW+
Sbjct: 363 NFSLTHLERYSPFQFVQHARERESCRRFVRMLGIKTPGVEQKVVNLSGGNQQKIVIAKWV 422

Query: 424 FSNPDVLILDEPTRGIDVGAKYEIYTIINQLAADGKGVLMISSEMPELLGNCDRIYVMNE 483
             +P VLI+DEPTRGIDVGAK E++ +I +LAA+G GV++ISS++ E+L   DRI  + E
Sbjct: 423 ARSPKVLIVDEPTRGIDVGAKAEVHALIARLAAEGIGVIVISSDLLEVLAVSDRILTVRE 482

Query: 484 GRIVAELPKGEASQESIM 501
           GRI  EL + +ASQE +M
Sbjct: 483 GRISGELSRAQASQEKVM 500



 Score = 74.3 bits (181), Expect = 9e-18
 Identities = 56/230 (24%), Positives = 108/230 (46%), Gaps = 10/230 (4%)

Query: 278 QVLHDINVTVRKGEVVGIAGLMGAGRTEFAMSVFGKSYGHRITGDVLIDGKPVDVSTVRK 337
           + L  +N+ +R GEV+ +AG  GAG++   M +    Y     G +L++G+ V ++    
Sbjct: 23  KALDRVNLEIRAGEVLALAGENGAGKSTL-MKILTGIYAPDPGGTILVEGQEVALADSHH 81

Query: 338 AIDAGLAYVTEDRKHLG-LVLNDNI-LHNTTLANLAGVSKASIIDDIKEMKVASDFRTRL 395
           A   G+  + ++   +G L + +NI L       L  + +  +  + +E+    D     
Sbjct: 82  ARTLGVNIIYQELAVVGNLTVGENIFLAREPRTRLGLIDRPRMYREAREVLATIDMDIDP 141

Query: 396 RIRSSGIFQETVNLSGGNQQKVVLSKWLFSNPDVLILDEPTRGIDVGAKYEIYTIINQLA 455
             R S        LS G QQ + ++K L +    +I+DEPT  +       +  I+ +L 
Sbjct: 142 ATRVS-------ELSVGQQQMIEIAKALCARSKAIIMDEPTASLSHHETSVLLGIVKRLR 194

Query: 456 ADGKGVLMISSEMPELLGNCDRIYVMNEGRIVAELPKGEASQESIMRAIM 505
                V+ IS  + E+    DR+ V+ +GR V   P  + ++E+++R ++
Sbjct: 195 ERNIAVVYISHRLEEIFELADRVTVLRDGRTVGTAPIADMTRETLVRLMV 244


Lambda     K      H
   0.316    0.135    0.374 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 624
Number of extensions: 34
Number of successful extensions: 9
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 512
Length of database: 505
Length adjustment: 34
Effective length of query: 478
Effective length of database: 471
Effective search space:   225138
Effective search space used:   225138
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources