GapMind for catabolism of small carbon sources

 

Aligments for a candidate for mglC in Paraburkholderia bryophila 376MFSha3.1

Align Putative beta-xyloside ABC transporter, permease component, component of Glucose porter. Also bind xylose (Boucher and Noll 2011). Induced by glucose (Frock et al. 2012). Directly regulated by glucose-responsive regulator GluR (characterized)
to candidate H281DRAFT_02714 H281DRAFT_02714 monosaccharide ABC transporter membrane protein, CUT2 family

Query= TCDB::G4FGN4
         (313 letters)



>lcl|FitnessBrowser__Burk376:H281DRAFT_02714 H281DRAFT_02714
           monosaccharide ABC transporter membrane protein, CUT2
           family
          Length = 331

 Score =  256 bits (653), Expect = 7e-73
 Identities = 134/309 (43%), Positives = 203/309 (65%), Gaps = 3/309 (0%)

Query: 7   KAREAGIFLILIAIVVFLGVTTREFLTVENIFTVILNVSFIAIMSFGMTMVIITSGIDLS 66
           + RE  +  +L+ + + + V +  FLT  N+  V  + S IA+MS GM +VIIT GIDLS
Sbjct: 24  RIRELNVLSVLLLVGLLISVFSPYFLTTNNLMGVFRSFSLIALMSIGMMLVIITGGIDLS 83

Query: 67  VGSILGAASVVMGLLMDEKGLSPFLSVVIGLAVGVGFGLANGLLITKARLAPFISTLGML 126
           VGS++G +S+V  L+  + G +   ++  GLAVG+  G  NG +IT  +L PFI+TLG L
Sbjct: 84  VGSVMGLSSLVTALVF-QHGYNAPAAIGAGLAVGIAVGAFNGFMITWIQLPPFIATLGTL 142

Query: 127 SVGRGLAYVMSGGWPISP-FPESFTVHGQGMVGPVPVPVIYMAVIGVIAHIFLKYTVTGR 185
           S+GRGL Y+++ G P++P  P+SFT  GQG +G VP PV+ +  +  +  + ++ T  GR
Sbjct: 143 SIGRGLMYIITKGVPVTPDVPDSFTFIGQGYIGFVPFPVVILLAMTAVFSVVMRQTRFGR 202

Query: 186 RIYAIGGNMEASKLVGIKTDRILILVYTINGFLAAFAGFLLTAWLGVAQPNAGQGYELDV 245
            +YA GGN  A++L G++T R+   VY ++G +A+ AG +  +    A+P +G G ELDV
Sbjct: 203 YVYATGGNEVAARLSGVRTARVKFTVYVLSGLIASMAGVIAFSRFVSAEPASGFGAELDV 262

Query: 246 IAATVIGGTSLSGGEGTILGAFLGAVIMGVLRNGMILLGVSSFWQQVVIGIVIIIAIAID 305
           IAA  IGG SLSGG G++ GA +GA + G++ NG++LL + ++ QQ + G VI+IA++ID
Sbjct: 263 IAAAAIGGASLSGGAGSVEGAIIGAALAGIITNGVVLLNIDTYAQQAITGCVILIAVSID 322

Query: 306 QIR-RAKER 313
             R R KER
Sbjct: 323 IWRVRRKER 331


Lambda     K      H
   0.328    0.145    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 315
Number of extensions: 24
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 313
Length of database: 331
Length adjustment: 28
Effective length of query: 285
Effective length of database: 303
Effective search space:    86355
Effective search space used:    86355
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory