GapMind for catabolism of small carbon sources

 

Alignments for a candidate for udh in Paraburkholderia bryophila 376MFSha3.1

Align uronate dehydrogenase (EC 1.1.1.203) (characterized)
to candidate H281DRAFT_03826 H281DRAFT_03826 uronate dehydrogenase

Query= BRENDA::Q88NN6
         (268 letters)



>FitnessBrowser__Burk376:H281DRAFT_03826
          Length = 283

 Score =  295 bits (756), Expect = 6e-85
 Identities = 145/263 (55%), Positives = 190/263 (72%), Gaps = 2/263 (0%)

Query: 5   PFNRLLLTGAAGGLGKVLRERLKGYAEVLRLSDISPMAPAAGPHEEVITCDLADKAAVHT 64
           PF RLLLTGAAG LG+ LR  L  +A+V+R++DI+ +   A PHEE    DLAD+ AV  
Sbjct: 15  PFRRLLLTGAAGNLGRQLRGVLAQWADVVRVTDIASLGEPA-PHEEASVVDLADREAVMQ 73

Query: 65  LVEGVDAIIHFGGVSTEHAFEEILGPNICGVFHVYEAARKHGVKRIIFASSNHTIGFYRQ 124
           LVEGVDAI+H GG+S +  F+++LG NI G +++YEAARKHGVKRI FASSNH IGF+  
Sbjct: 74  LVEGVDAIVHLGGISIDAPFDDLLGANIAGTYNLYEAARKHGVKRIAFASSNHVIGFHPV 133

Query: 125 DERIDAHAPRRPDSYYGLSKCYGEDVASFYFDRYGIETVSIRIGSSFPQPQNLRMLCTWL 184
            E +DA AP+RPD+ YG++KC+GE ++ +Y+DR+GIETV +RIGSSF +P+N RML T+L
Sbjct: 134 TEVLDADAPQRPDTLYGVTKCFGEALSRYYYDRFGIETVCMRIGSSFDEPKNSRMLVTYL 193

Query: 185 SYDDLVQLIERGLFTPGVGHTIVYGASDNRTVWWDNRHAAHLGYVPKDSSETFRAAVEA- 243
           SY D ++L+   LFT  VGH +VYGASDN   WWDN   + LG+ P+DSS  F A   A 
Sbjct: 194 SYRDFIELVRCSLFTNRVGHVVVYGASDNPVKWWDNTKVSFLGFNPRDSSAPFAARFPAT 253

Query: 244 QPAPAADDPSMVYQGGAFAVAGP 266
            P  + DDP+  +QGG F +  P
Sbjct: 254 APDASRDDPAQRFQGGPFVLGEP 276


Lambda     K      H
   0.321    0.138    0.427 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 235
Number of extensions: 9
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 268
Length of database: 283
Length adjustment: 25
Effective length of query: 243
Effective length of database: 258
Effective search space:    62694
Effective search space used:    62694
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory