Align ABC transporter for L-Glutamine, L-Histidine, and other L-amino acids, ATPase component (characterized)
to candidate H281DRAFT_02613 H281DRAFT_02613 amino acid ABC transporter ATP-binding protein, PAAT family (TC 3.A.1.3.-)
Query= reanno::Smeli:SMc02121 (258 letters) >FitnessBrowser__Burk376:H281DRAFT_02613 Length = 249 Score = 243 bits (620), Expect = 3e-69 Identities = 123/238 (51%), Positives = 162/238 (68%) Query: 19 IEITNMNKWYGDFHVLRDINLRVMRGERIVVAGPSGSGKSTMIRCINRLEEHQKGKIVVD 78 ++ + K +G VL ++L + GE +V+ GPSGSGKST++RCIN LE+ G +VVD Sbjct: 11 VQFKGVTKRFGKTVVLDRVDLNIGAGEVVVLIGPSGSGKSTLLRCINALEQIDGGDLVVD 70 Query: 79 GIELTNDLKKIDEVRREVGMVFQHFNLFPHLTILENCTLAPIWVRKMPKKEAEQVAMHFL 138 GI + KK+ E+R+E GMVFQ FNLFP +T LEN P VR M K EAE +A L Sbjct: 71 GISVLGGAKKMREIRQEAGMVFQQFNLFPQMTALENVAFGPRQVRGMSKSEAESLARELL 130 Query: 139 ERVKIPEQALKYPGQLSGGQQQRVAIARSLCMRPKILLFDEPTSALDPEMVKEVLDTMVG 198 +V + + Y +LSGGQQQRVAIAR+L ++PK++LFDEPTSALDPE+ +EVL M Sbjct: 131 TKVGLGSRVGHYACELSGGQQQRVAIARALAVKPKLMLFDEPTSALDPELRQEVLRVMQA 190 Query: 199 LAEEGMTMICVTHEMGFARQVANRVIFMDQGQIVEQNSPAEFFDNPQHERTKLFLSQI 256 LAEEGM+M+ VTHEM FAR+V R+IFM+ G I PAE DNP ++R + FL + Sbjct: 191 LAEEGMSMVVVTHEMAFARKVGTRLIFMEHGHITVDGPPAELLDNPPNQRLRDFLQHV 248 Lambda K H 0.322 0.136 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 188 Number of extensions: 6 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 258 Length of database: 249 Length adjustment: 24 Effective length of query: 234 Effective length of database: 225 Effective search space: 52650 Effective search space used: 52650 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory