Align Acetyl-CoA acetyltransferase; Acetoacetyl-CoA thiolase; Beta-ketothiolase; EC 2.3.1.9 (characterized)
to candidate H281DRAFT_00852 H281DRAFT_00852 acetyl-CoA acetyltransferase
Query= SwissProt::P14611 (393 letters) >FitnessBrowser__Burk376:H281DRAFT_00852 Length = 393 Score = 669 bits (1725), Expect = 0.0 Identities = 337/392 (85%), Positives = 365/392 (93%) Query: 1 MTDVVIVSAARTAVGKFGGSLAKIPAPELGAVVIKAALERAGVKPEQVSEVIMGQVLTAG 60 MTDVVIVSAARTAVGKFGGSLAKI APELGA VI+A LERAG+KPEQVSEVI+GQVLTAG Sbjct: 1 MTDVVIVSAARTAVGKFGGSLAKIAAPELGATVIRAVLERAGMKPEQVSEVILGQVLTAG 60 Query: 61 SGQNPARQAAIKAGLPAMVPAMTINKVCGSGLKAVMLAANAIMAGDAEIVVAGGQENMSA 120 SGQNPARQ+ IKAGLPA VP MTINKVCGSGLKAVMLAANAI+AGDA+IV+AGGQENMSA Sbjct: 61 SGQNPARQSLIKAGLPAAVPGMTINKVCGSGLKAVMLAANAIIAGDADIVIAGGQENMSA 120 Query: 121 APHVLPGSRDGFRMGDAKLVDTMIVDGLWDVYNQYHMGITAENVAKEYGITREAQDEFAV 180 APHVLPGSRDGFRMGDAKL+D+MIVDGLWDVYNQYHMG+TAENVAKE+ ITRE QD FA Sbjct: 121 APHVLPGSRDGFRMGDAKLIDSMIVDGLWDVYNQYHMGVTAENVAKEFDITREQQDAFAA 180 Query: 181 GSQNKAEAAQKAGKFDEEIVPVLIPQRKGDPVAFKTDEFVRQGATLDSMSGLKPAFDKAG 240 SQNKAEAAQK+G+FD+EIVPV IPQRKGDPV F TDEFVR G T +S++GLKPAF K G Sbjct: 181 LSQNKAEAAQKSGRFDDEIVPVEIPQRKGDPVRFATDEFVRHGVTAESLAGLKPAFSKEG 240 Query: 241 TVTAANASGLNDGAAAVVVMSAAKAKELGLTPLATIKSYANAGVDPKVMGMGPVPASKRA 300 TVTAANASGLNDGAAAV+VMSA KA+ LGLTPLA IK+YANAGVDPKVMGMGPVPAS+R Sbjct: 241 TVTAANASGLNDGAAAVLVMSAKKAEALGLTPLARIKAYANAGVDPKVMGMGPVPASRRC 300 Query: 301 LSRAEWTPQDLDLMEINEAFAAQALAVHQQMGWDTSKVNVNGGAIAIGHPIGASGCRILV 360 L RA W+ DLDLMEINEAFAAQALAVH+QMGWDTSK+NVNGGAIAIGHPIGASGCRILV Sbjct: 301 LERAGWSVNDLDLMEINEAFAAQALAVHKQMGWDTSKINVNGGAIAIGHPIGASGCRILV 360 Query: 361 TLLHEMKRRDAKKGLASLCIGGGMGVALAVER 392 TLL+EM++RDAKKGLASLCIGGGMGVALA+ER Sbjct: 361 TLLYEMQKRDAKKGLASLCIGGGMGVALALER 392 Lambda K H 0.315 0.131 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 599 Number of extensions: 18 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 393 Length of database: 393 Length adjustment: 31 Effective length of query: 362 Effective length of database: 362 Effective search space: 131044 Effective search space used: 131044 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 50 (23.9 bits)
Align candidate H281DRAFT_00852 H281DRAFT_00852 (acetyl-CoA acetyltransferase)
to HMM TIGR01930 (acetyl-CoA C-acyltransferase (EC 2.3.1.16))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01930.hmm # target sequence database: /tmp/gapView.644.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01930 [M=385] Accession: TIGR01930 Description: AcCoA-C-Actrans: acetyl-CoA C-acyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.5e-155 503.1 7.8 2.8e-155 502.9 7.8 1.0 1 lcl|FitnessBrowser__Burk376:H281DRAFT_00852 H281DRAFT_00852 acetyl-CoA acety Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Burk376:H281DRAFT_00852 H281DRAFT_00852 acetyl-CoA acetyltransferase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 502.9 7.8 2.8e-155 2.8e-155 1 385 [] 6 391 .. 6 391 .. 0.99 Alignments for each domain: == domain 1 score: 502.9 bits; conditional E-value: 2.8e-155 TIGR01930 1 ivdavRtpigklggslkelsaedLlaavikelleragldpekidevilGnvlqageqaniaReaal 66 iv+a+Rt++gk+ggsl+++ a +L+a+vi+++lerag++pe++ evilG+vl+ag+++n+aR+ ++ lcl|FitnessBrowser__Burk376:H281DRAFT_00852 6 IVSAARTAVGKFGGSLAKIAAPELGATVIRAVLERAGMKPEQVSEVILGQVLTAGSGQNPARQSLI 71 8***************************************************************** PP TIGR01930 67 aaglpesvpaltvnrvCaSglqAvalaaqkikaGeadvvvaGGvEsmSrvpillkaslrreslklg 132 +aglp vp++t+n+vC+Sgl+Av+laa++i aG+ad+v+aGG E+mS +p++l+ s r+++++g lcl|FitnessBrowser__Burk376:H281DRAFT_00852 72 KAGLPAAVPGMTINKVCGSGLKAVMLAANAIIAGDADIVIAGGQENMSAAPHVLPGS--RDGFRMG 135 *******************************************************98..9****** PP TIGR01930 133 kakledqllkdl...vktklsmgetAenlakkygisReeqDeyalrShqkaakAieegkfkdeivp 195 +akl d+++ d+ v+++++mg+tAen+ak+++i+Re+qD++a+ S++ka++A+++g+f+deivp lcl|FitnessBrowser__Burk376:H281DRAFT_00852 136 DAKLIDSMIVDGlwdVYNQYHMGVTAENVAKEFDITREQQDAFAALSQNKAEAAQKSGRFDDEIVP 201 ***************99************************************************* PP TIGR01930 196 vevkgk...kkvvskDegirpnttlekLakLkpafkekkgstvtAgNssqlnDGAaalllmseeva 258 ve++++ ++ +++De +r ++t+e+La+Lkpaf + +g tvtA+N+s+lnDGAaa+l+ms ++a lcl|FitnessBrowser__Burk376:H281DRAFT_00852 202 VEIPQRkgdPVRFATDEFVRHGVTAESLAGLKPAFSK-EG-TVTAANASGLNDGAAAVLVMSAKKA 265 ****999*999************************95.8*.6************************ PP TIGR01930 259 kelgltplarivsaavagvdpeemglgpvpAiekaLkkaglsisdidlvEinEAFAaqvlavekel 324 ++lgltplari ++a+agvdp++mg+gpvpA++++L++ag+s++d+dl+EinEAFAaq+lav k++ lcl|FitnessBrowser__Burk376:H281DRAFT_00852 266 EALGLTPLARIKAYANAGVDPKVMGMGPVPASRRCLERAGWSVNDLDLMEINEAFAAQALAVHKQM 331 ****************************************************************** PP TIGR01930 325 gsldlekvNvnGGAiAlGHPlGasGarivltllkeLkergkkyGlatlCvggGqGaAvile 385 g d++k+NvnGGAiA+GHP+GasG+ri++tll+e+++r++k Gla+lC+ggG+G+A+ le lcl|FitnessBrowser__Burk376:H281DRAFT_00852 332 G-WDTSKINVNGGAIAIGHPIGASGCRILVTLLYEMQKRDAKKGLASLCIGGGMGVALALE 391 *.88*****************************************************9886 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (385 nodes) Target sequences: 1 (393 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.02 # Mc/sec: 7.48 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory