Align D-galactono-lactonase (EC 3.1.1.-) (characterized)
to candidate H281DRAFT_00300 H281DRAFT_00300 6-phosphogluconolactonase
Query= reanno::pseudo13_GW456_L13:PfGW456L13_3314 (389 letters) >FitnessBrowser__Burk376:H281DRAFT_00300 Length = 434 Score = 323 bits (827), Expect = 8e-93 Identities = 162/364 (44%), Positives = 241/364 (66%), Gaps = 7/364 (1%) Query: 26 YQLLVGSYTAGQSQGIYRLAFDSRTGQIDASPLQVIKSANPSWLTLSKDQRHLFVVNENG 85 Y +LVG+YT G+S+G+Y FD++TG DA+ + V ++ NPS+L +S+D+R ++ VNE Sbjct: 72 YDMLVGTYTGGKSEGLYVYRFDTKTG--DATRVSVAQTVNPSYLVVSRDRRFVYAVNEL- 128 Query: 86 PGQTDPV---GRVSSFAIDPKTHALSLISQVQSLGNEPTHSSLSIDGSHLFVSNYSVAED 142 PG P G +S+F D + LS +++V + GN+P + SLS DG +L +NYSVA D Sbjct: 129 PGDNGPASQRGGISAFRFDAASGQLSFLNKVSADGNDPCYLSLSPDGKYLLTANYSVAAD 188 Query: 143 PGGTLAVLPVAADGKLKAVVQMSSHPASRVNPERQASAHVHSTIPSPDGRYVFANDLGAD 202 PGG+ AV PV ADG+L A V H RQ ++HVHST+ SPDGRY+FA DLGAD Sbjct: 189 PGGSFAVFPVQADGQLGASVLTVHHEGGGPVKGRQDNSHVHSTVFSPDGRYLFAQDLGAD 248 Query: 203 KVFAYRFDPKANPELPLTPATPAFVQLPPGSGPRHLLFSADGKHAWLTMEMSAQVAVFDY 262 K+++YR+ P + L P + Q PG+GPRHL+F DGKHA+LT E++A V++F+Y Sbjct: 249 KLYSYRYTPDGSRGL-FGPTDWRYTQEKPGTGPRHLVFGVDGKHAYLTSELAATVSIFNY 307 Query: 263 HDGQLEQTQMVDLAAGQPVSDKAAAALHASADGKFLYVSNRGTANQLLVFAIDPATGHLS 322 DG+L Q Q V L AAA+H S DG+FLY +NRG AN++++F++DP GHL Sbjct: 308 DDGKLTQVQTVSLTEPGFKGAVGAAAIHLSPDGRFLYATNRGDANEIVIFSVDPTNGHLK 367 Query: 323 ELQRRAVEGDHPREFSLDPSGKFLLIANQKSNQIVVVERDARTGLLGKTVQKLPMDAPSD 382 ++ ++ G PREF++DP+GK+L++ NQ S+ + V RD ++GLL +++ + +P D Sbjct: 368 KIGHQSSLGKSPREFAIDPTGKWLIVGNQNSDTVYVFRRDPQSGLLEANPKRIEIGSPVD 427 Query: 383 LRFL 386 + + Sbjct: 428 FKLV 431 Lambda K H 0.316 0.132 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 658 Number of extensions: 53 Number of successful extensions: 9 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 389 Length of database: 434 Length adjustment: 31 Effective length of query: 358 Effective length of database: 403 Effective search space: 144274 Effective search space used: 144274 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory