GapMind for catabolism of small carbon sources

 

Alignments for a candidate for ilvE in Paraburkholderia bryophila 376MFSha3.1

Align aromatic-amino-acid transaminase TyrB; EC 2.6.1.57 (characterized)
to candidate H281DRAFT_02875 H281DRAFT_02875 aromatic-amino-acid transaminase

Query= CharProtDB::CH_004054
         (397 letters)



>FitnessBrowser__Burk376:H281DRAFT_02875
          Length = 399

 Score =  448 bits (1152), Expect = e-130
 Identities = 222/397 (55%), Positives = 289/397 (72%), Gaps = 1/397 (0%)

Query: 1   MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIPQLQAVAEAEARLNAQP 60
           MF+ + AY GDPIL+L E F+ DPR +KVNLSIG+Y+++ G +P + AV  AE  L    
Sbjct: 1   MFEHIPAYPGDPILSLNEDFQLDPRQNKVNLSIGIYFDDAGKLPVMDAVRRAETAL-LDA 59

Query: 61  HGASLYLPMEGLNCYRHAIAPLLFGADHPVLKQQRVATIQTLGGSGALKVGADFLKRYFP 120
            G   YLPM GL  YR     L+FG D       R+AT+QT+GGSGALKVGADFL+RYFP
Sbjct: 60  IGPRSYLPMAGLPLYRETAQALVFGEDSEARAAGRIATLQTIGGSGALKVGADFLRRYFP 119

Query: 121 ESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRFNDLLATLKTLPARSIVLLHP 180
            S +W+SDP+WENH  +F GAG  V+TYP+YDE T G+RF D++ T+  LP RS+VLLH 
Sbjct: 120 GSQMWISDPSWENHRVVFEGAGLTVNTYPYYDEQTGGLRFADMIDTIDRLPERSVVLLHA 179

Query: 181 CCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGFGAGMEEDAYAIRAIASAGLPAL 240
           CCHNPTG DL+ +QW  ++ +L+ R+LI F+D+AYQGFGAG+EEDA  +R +A AG+P +
Sbjct: 180 CCHNPTGVDLSVEQWTELVPVLQRRKLIAFVDMAYQGFGAGLEEDAACVRMLAEAGVPLI 239

Query: 241 VSNSFSKIFSLYGERVGGLSVMCEDAEAAGRVLGQLKATVRRNYSSPPNFGAQVVAAVLN 300
            +NSFSK FSLYGER G LSV+C++A+ A RVLGQL + VR NYS+PP  GA++VA VL+
Sbjct: 240 AANSFSKNFSLYGERCGALSVVCKNADEASRVLGQLMSAVRANYSNPPTHGARLVANVLS 299

Query: 301 DEALKASWLAEVEEMRTRILAMRQELVKVLSTEMPERNFDYLLNQRGMFSYTGLSAAQVD 360
           D +L+ASW AE+  MR RILAMR  +   L+  + E      + QRGMF+YTGL+A+QV+
Sbjct: 300 DVSLRASWEAELATMRERILAMRGTIHAGLAGRVDEVMRARYVAQRGMFTYTGLTASQVE 359

Query: 361 RLREEFGVYLIASGRMCVAGLNTANVQRVAKAFAAVM 397
           RLR+E  VY++ SGRMCVAGLN  N   VA + AAV+
Sbjct: 360 RLRDEHAVYVLRSGRMCVAGLNARNADYVASSIAAVV 396


Lambda     K      H
   0.320    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 488
Number of extensions: 11
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 397
Length of database: 399
Length adjustment: 31
Effective length of query: 366
Effective length of database: 368
Effective search space:   134688
Effective search space used:   134688
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory