Alignments for a candidate for amaB in Paraburkholderia bryophila 376MFSha3.1

GapMind for catabolism of small carbon sources

Alignments for a candidate for amaB in Paraburkholderia bryophila 376MFSha3.1

Align Putative aldehyde dehydrogenase transmembrane protein; EC 1.2.1.3 (characterized, see rationale)
to candidate H281DRAFT_01833 H281DRAFT_01833 aldehyde dehydrogenase (NAD+)

Query= uniprot:Q92L07
         (510 letters)



>FitnessBrowser__Burk376:H281DRAFT_01833
          Length = 499

 Score =  659 bits (1700), Expect = 0.0
 Identities = 329/498 (66%), Positives = 395/498 (79%), Gaps = 2/498 (0%)

Query: 13  EAAALLDKMGVAKDLYTGGDMPSFSPVTGEKIASLKTVSAAEAAGKIEKADEAFRAWRLV 72
           +A+A+L ++G++  L   GD+   SP+ GE I  + + + AE    +  A +A+ +WR V
Sbjct: 2   KASAILSELGISH-LAEAGDIAVHSPINGELIGRVASRTVAEVDAALASAQKAYASWRNV 60

Query: 73  PAPKRGELVRLLGEELRAFKADLGRLVSIEAGKIPSEGLGEVQEMIDICDFAVGLSRQLY 132
           PAP+RGELVRLLG +LR  K  LG ++++E GKI  EGLGEVQEMIDICDFAVGLSRQLY
Sbjct: 61  PAPRRGELVRLLGNKLREQKHALGSIITLETGKILQEGLGEVQEMIDICDFAVGLSRQLY 120

Query: 133 GLTIATERPGHRMMETWHPLGVVGIISAFNFPVAVWSWNAALALVCGDAVVWKPSEKTPL 192
           GLTIA+ERPGHRM ETWHPLGV  +ISAFNFP AVWSWNAALALVCG+AVVWKPSEKTPL
Sbjct: 121 GLTIASERPGHRMAETWHPLGVCTVISAFNFPAAVWSWNAALALVCGNAVVWKPSEKTPL 180

Query: 193 TALACQAILERAIARFGDAPEGLSQVLIGDRAIGEVLVDHPKVPLVSATGSTRMGREVGP 252
           TALA   IL+ A+  FGDAPEGL+ V+ G R +G  LV  P+  +VSATGST MGR VG 
Sbjct: 181 TALAVDKILQDALKEFGDAPEGLTSVVNGGREVGAKLVADPRSNIVSATGSTEMGRAVGV 240

Query: 253 RLAKRFARAILELGGNNAGIVCPSADLDMALRAIAFGAMGTAGQRCTTLRRLFVHESVYD 312
            +A+RF R+ILELGGNNAGIV  SAD+++ALR I F A+GTAGQRCT+LRRLFVHESVYD
Sbjct: 241 EVARRFGRSILELGGNNAGIVSGSADMELALRGIVFSAVGTAGQRCTSLRRLFVHESVYD 300

Query: 313 QLVPRLKKAYQSVSVGNPLESAALVGPLVDKAAFDGMQKAIAEAKNHGGAVTGGER-VEL 371
           + V RLK  Y  V +GNPLE   L+GPL+D+ +F+ MQ A+ +AK+ GG V GGER    
Sbjct: 301 KAVERLKALYSKVVIGNPLEQGVLMGPLIDEQSFNRMQAALEQAKSEGGKVFGGERHAVA 360

Query: 372 GHENGYYVKPALVEMPKQEGPVLEETFAPILYVMKYSDFDAVLAEHNAVAAGLSSSIFTR 431
           G+E G+YV+PA+VEMP Q   VL+ETFAPILYV+KYSDFD  +  +NA   GLSS +FT 
Sbjct: 361 GNEKGFYVRPAIVEMPSQTSVVLKETFAPILYVLKYSDFDDAIGGNNAAVHGLSSCVFTT 420

Query: 432 DMQESERFLAADGSDCGIANVNIGTSGAEIGGAFGGEKETGGGRESGSDAWKAYMRRATN 491
           D++E+ERFL+A GSDCGIANVNIG SGAEIGGAFGGEKETGGGRESGSD+WKAYMRRATN
Sbjct: 421 DLREAERFLSASGSDCGIANVNIGPSGAEIGGAFGGEKETGGGRESGSDSWKAYMRRATN 480

Query: 492 TVNYSKALPLAQGVSFDI 509
           TVNYS ALPLAQG+ F+I
Sbjct: 481 TVNYSSALPLAQGIDFNI 498


Lambda     K      H
   0.317    0.134    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 778
Number of extensions: 33
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 510
Length of database: 499
Length adjustment: 34
Effective length of query: 476
Effective length of database: 465
Effective search space:   221340
Effective search space used:   221340
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources