Alignments for a candidate for malK_Ss in Paraburkholderia bryophila 376MFSha3.1

GapMind for catabolism of small carbon sources

Alignments for a candidate for malK_Ss in Paraburkholderia bryophila 376MFSha3.1

Align MalK, component of Maltose and maltooligosaccharide porter (characterized)
to candidate H281DRAFT_01032 H281DRAFT_01032 peptide/nickel transport system ATP-binding protein

Query= TCDB::Q97UG5
         (617 letters)



>FitnessBrowser__Burk376:H281DRAFT_01032
          Length = 340

 Score =  197 bits (501), Expect = 6e-55
 Identities = 105/297 (35%), Positives = 174/297 (58%), Gaps = 5/297 (1%)

Query: 28  VTNVNFEVYPNEIFAIAGESGCGKSTLAMAIYGLLKYPGVV-LRGHVYLKDKDILSITQE 86
           V  V+F V+  E   + GESGCGKS  ++A+ GLL  P    + G  + +  D+  +++ 
Sbjct: 29  VDRVSFLVHAGETLCVVGESGCGKSVTSLALMGLLASPPARRVAGTAHFEGVDLFGLSER 88

Query: 87  ELRKLRMKEFVYVPQFAMDALDPVAKIGDQMMRAAVSH-GVNVEEARKLIKEKLELVDLP 145
           EL  +R      + Q  M +L+P   +G+Q+  +   H GV+   AR+   + L LV +P
Sbjct: 89  ELADIRGNRMSMIFQEPMTSLNPAYTVGEQIGESIRRHRGVSRAAAREEALKMLRLVHIP 148

Query: 146 YNVVNM--YPHELSGGMRQRVVIATSILLNPSLIILDEPTTGLDVIVQYEILKDLKRIQR 203
                +  + H+LSGGMRQRV+IA ++  +P L+I DEPTT LDV +Q ++L  ++ +Q+
Sbjct: 149 APETRLDAFAHQLSGGMRQRVMIAMALANSPRLLIADEPTTALDVTIQAQVLALVRNLQK 208

Query: 204 QLGVSLVIISHDISMLLMISDRVGIMYAGEIVEIGSKEEIIKRPSHPYTYLLISSLPSLV 263
           + G ++++I+HD+ ++  ++D V +MYAG+IVE G+  ++   P HPYT  L+ ++PS+ 
Sbjct: 209 ETGTAMLLITHDLGVVAEVADHVAVMYAGKIVEYGTVSDLFDDPQHPYTIGLMGAIPSVG 268

Query: 264 KRREKLLSIPGNPPLMLSKVPNSCRFYDRCPFKMEKCSTLNPALGDIMDGHKARCFL 320
           KR   L +I G+ P    ++P  CRF  RCPF  ++C    P    +   H+  C+L
Sbjct: 269 KREGSLATIRGSVPTP-EQMPRGCRFAPRCPFAEQRCIDAAPPERTLEGEHRVACWL 324



 Score =  142 bits (357), Expect = 3e-38
 Identities = 87/245 (35%), Positives = 139/245 (56%), Gaps = 8/245 (3%)

Query: 374 LSEPINAVNDVSFELKKGTITALVGGSGHGKSTIAKILAGMI-----QQTSGKIILLGKD 428
           L +  N V+ VSF +  G    +VG SG GKS  +  L G++     ++ +G     G D
Sbjct: 22  LPDAPNVVDRVSFLVHAGETLCVVGESGCGKSVTSLALMGLLASPPARRVAGTAHFEGVD 81

Query: 429 VSEYGVRNSMWYKEN-VQMIFQDPYSSLDPRHTVRWHVERPLLIHKKVSNKDQLLPKIIE 487
           +     R     + N + MIFQ+P +SL+P +TV   +   +  H+ VS +     + ++
Sbjct: 82  LFGLSERELADIRGNRMSMIFQEPMTSLNPAYTVGEQIGESIRRHRGVS-RAAAREEALK 140

Query: 488 VLKNVGLKPPEKYLYKYPHELSGGERQRVAIARATAVEPKVLVADEPVSMLDASLRAGIL 547
           +L+ V +  PE  L  + H+LSGG RQRV IA A A  P++L+ADEP + LD +++A +L
Sbjct: 141 MLRLVHIPAPETRLDAFAHQLSGGMRQRVMIAMALANSPRLLIADEPTTALDVTIQAQVL 200

Query: 548 NLIKKF-KKNGISILYITHDIATVNYIADEIMVIYKGRIVEKGNTYEVISNPSHEYTKRL 606
            L++   K+ G ++L ITHD+  V  +AD + V+Y G+IVE G   ++  +P H YT  L
Sbjct: 201 ALVRNLQKETGTAMLLITHDLGVVAEVADHVAVMYAGKIVEYGTVSDLFDDPQHPYTIGL 260

Query: 607 IEAVP 611
           + A+P
Sbjct: 261 MGAIP 265


Lambda     K      H
   0.319    0.138    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 415
Number of extensions: 25
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 617
Length of database: 340
Length adjustment: 33
Effective length of query: 584
Effective length of database: 307
Effective search space:   179288
Effective search space used:   179288
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources