Align ABC transporter for D-mannitol and D-mannose, ATPase component (characterized)
to candidate H281DRAFT_02629 H281DRAFT_02629 carbohydrate ABC transporter ATP-binding protein, CUT1 family (TC 3.A.1.1.-)
Query= reanno::pseudo3_N2E3:AO353_25895 (367 letters) >FitnessBrowser__Burk376:H281DRAFT_02629 Length = 381 Score = 371 bits (952), Expect = e-107 Identities = 195/372 (52%), Positives = 269/372 (72%), Gaps = 12/372 (3%) Query: 1 MAHLKIKNLQKGFEGFS-IIKGIDLEVNDREFVVFVGPSGCGKSTLLRLIAGLEEVTAGT 59 MA + ++ +QK F+G + +++ ++LE+ + EF VF+GPSGCGKSTLLR++AGLE+ T G Sbjct: 1 MAAISLRGIQKTFDGNAPVLRDLNLEIGEHEFCVFLGPSGCGKSTLLRIVAGLEDQTDGD 60 Query: 60 IELDGRDITEVSPAKRDLAMVFQTYALYPHMSVRKNMSFALDLAGVNKAEVEKKVNEAAR 119 + + GR + V A+R +AMVFQ+YAL+PHM+V +NM+F L LA KAE+++KV EAA Sbjct: 61 VSIGGRTMNSVPAAERGVAMVFQSYALFPHMTVYENMAFGLKLARTPKAEIDRKVREAAH 120 Query: 120 ILELGPMLERKPKQLSGGQRQRVAIGRAIVRNPKIFLFDEPLSNLDAALRVQMRLELARL 179 +L+L +L RKP++LSGGQRQRVAIGRAIVR P +FLFDEPLSNLDAALR Q R+E+ARL Sbjct: 121 VLQLDALLARKPRELSGGQRQRVAIGRAIVREPGVFLFDEPLSNLDAALRGQTRIEIARL 180 Query: 180 HKE-LQATMIYVTHDQVEAMTLADKVVVLNG-------GRIEQVGSPLELYHQPANLFVA 231 H+ QA+++YVTHDQ+EAMTLADK+V+L+ G I Q+G+PLELYH+P + FVA Sbjct: 181 HQRYAQASVVYVTHDQIEAMTLADKIVLLHAGAETQRLGSIAQIGAPLELYHRPRSKFVA 240 Query: 232 GFLGTPKMGFLKGKVTRVERQNCEVLLDAGTRITLPLSGANLSIGGAVTLGIRPEHLNLA 291 GF+G+P+M F+ G V R+ + EV + GT + + + G L G VTLGIRPEH+ Sbjct: 241 GFIGSPRMNFIDGVVERIADDSVEVSM-PGTALRVLVDGRRLKPGQRVTLGIRPEHVRFD 299 Query: 292 LPGDCTLQVTADVSERLGSDTFCHVLTASGEALTMRIRGDLASRYGEQLSLHLDAEHCHL 351 G TL V A ++E+LG ++ H A G L + GDL R GE+L+LHL A+ CHL Sbjct: 300 -GGTQTLAVNALLTEQLGEHSYLHADHAGG-TLIAKAPGDLHVRNGERLALHLPADACHL 357 Query: 352 FDANGVAVARPL 363 FD +G+A+ R + Sbjct: 358 FDEDGIALHRTI 369 Lambda K H 0.321 0.137 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 409 Number of extensions: 17 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 367 Length of database: 381 Length adjustment: 30 Effective length of query: 337 Effective length of database: 351 Effective search space: 118287 Effective search space used: 118287 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory