GapMind for catabolism of small carbon sources

 

Aligments for a candidate for HSERO_RS03645 in Paraburkholderia bryophila 376MFSha3.1

Align ABC-type sugar transport system, permease component protein (characterized, see rationale)
to candidate H281DRAFT_04148 H281DRAFT_04148 monosaccharide ABC transporter membrane protein, CUT2 family

Query= uniprot:D8IZC8
         (344 letters)



>lcl|FitnessBrowser__Burk376:H281DRAFT_04148 H281DRAFT_04148
           monosaccharide ABC transporter membrane protein, CUT2
           family
          Length = 335

 Score =  220 bits (560), Expect = 5e-62
 Identities = 122/286 (42%), Positives = 180/286 (62%), Gaps = 8/286 (2%)

Query: 57  TSNFASAENTMNILRQVAINLVLAAGMTFVILTAGIDLSVGSVLAVSAVLG---MQVSLG 113
           + +F S  N  N+LRQV+IN ++A GMT VILT GIDLSVGSV+A++  L    M   + 
Sbjct: 54  SDSFLSGANIENVLRQVSINAIIAVGMTCVILTGGIDLSVGSVMALAGTLAAGLMVAGMN 113

Query: 114 AAPGWAIPMFIFSGLVMGMVNGAMVALLNINAFVVTLGTMTAFRGAAYLLADGTTVLNND 173
           A    A+ + +  GL  G  NG  VA   +   +VTL TM   RG A +   G  +  + 
Sbjct: 114 ALAALAVGVAV--GLGFGAANGFFVAFAGMPPIIVTLATMGIARGLALIYTGGYPI--DG 169

Query: 174 IPSF-EWIGNGDFLHVPWLIWVAVAVVLLSWVILRKTVLGMHIYAIGGNLQAARLTGIRV 232
           +P +  + G+G  L +   + +   + +++WV+L +   G ++YAIGGN QA RL+G+RV
Sbjct: 170 LPDWVSFFGSGKILGIQAPVVIMAVIYVIAWVLLERMPFGRYVYAIGGNEQATRLSGVRV 229

Query: 233 GLVLLFVYSISGLFSGLAGAMSASRLYGANGNWGSGYELDAIAAVVLGGTSLMGGVGSIW 292
             V L VY+I+GL S  A  +  +RL     N G G+ELDAIAAVV+GGTS+ GG GSI 
Sbjct: 230 ARVKLIVYTIAGLTSSFAAIVLTARLMSGQPNAGVGFELDAIAAVVMGGTSISGGRGSII 289

Query: 293 GTVVGALIIGVMNNGLTILGLSSFWQYVAKGAVIVLAVILDKWRQK 338
           GT++GAL++GV+NNGL ++G++ + Q V KG +I+LA+ + + R+K
Sbjct: 290 GTLIGALLLGVLNNGLNMVGVNPYVQNVIKGGIILLAIYISRDRRK 335


Lambda     K      H
   0.324    0.138    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 331
Number of extensions: 21
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 344
Length of database: 335
Length adjustment: 28
Effective length of query: 316
Effective length of database: 307
Effective search space:    97012
Effective search space used:    97012
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory