Align Sugar transport system permease protein aka TT_C0326, component of The glucose/mannose porter TTC0326-8 plus MalK1 (ABC protein, shared with 3.A.1.1.25) (characterized)
to candidate H281DRAFT_01452 H281DRAFT_01452 carbohydrate ABC transporter membrane protein 2, CUT1 family
Query= TCDB::Q72KX4 (268 letters) >FitnessBrowser__Burk376:H281DRAFT_01452 Length = 305 Score = 213 bits (543), Expect = 3e-60 Identities = 114/278 (41%), Positives = 175/278 (62%), Gaps = 13/278 (4%) Query: 3 RALLYGFLLLMAGFFLLPVYLVVLTALKEPARITLETVWQWPHPPYWESFRTAWEA---- 58 R +Y FLL A FFLLP+Y++++T+ K + I L + P ++ AWE+ Sbjct: 29 RVGVYLFLLSAALFFLLPLYVMLVTSFKPMSEIRLGNLLALPAHFTLHAWSAAWESACTG 88 Query: 59 -----FRPKFQNSVVLAVSATLLSALVGSLNGYVLAKWPFRGSGLLFALILFGMFIPYQS 113 + F NSV + V +T+ S +G++NGY L+ W RG+G+LF ++L G FIP Q Sbjct: 89 LDCNGIQVGFWNSVRIVVPSTVFSIAIGAVNGYALSFWRPRGAGVLFGVLLMGAFIPVQV 148 Query: 114 ILIPLFQFMKSIGLYGSLFGLVLVHVIYGIPIVTLIFRNYYSEIPDELVEAARIDGAGFF 173 ++ PL + + S+ L+ SL G+V++H I+G+P++TL+FRNYY+ IP EL +AARIDG GF+ Sbjct: 149 MVYPLVRVLASVHLFSSLPGIVVIHTIFGMPVMTLLFRNYYASIPQELFKAARIDGGGFW 208 Query: 174 GIFRHVILPLSVPAFVVVAIWQFTQIWNEFLFAVTLTRPESQPITVALAQLAG---GEAV 230 IF ++LP+S P VV I Q T IWN+F+ + ++ P+TV L + GE + Sbjct: 209 RIFVQLMLPMSTPIIVVAVIMQVTGIWNDFILGLVFAGTKNLPMTVQLNNIINTTTGERL 268 Query: 231 KWNLPMAGAILAALPTLLVYILLGRYFLRGLLAGSVKG 268 +N+ MA IL +L L VY + GR+F+RG+ +G+VKG Sbjct: 269 -YNVNMAATILTSLVPLAVYFISGRWFVRGIASGAVKG 305 Lambda K H 0.330 0.146 0.461 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 272 Number of extensions: 12 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 268 Length of database: 305 Length adjustment: 26 Effective length of query: 242 Effective length of database: 279 Effective search space: 67518 Effective search space used: 67518 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory