Align 5-dehydro-2-deoxygluconokinase (EC 2.7.1.92); possible 5-dehydro-2-deoxyphosphogluconate aldolase DUF2090 (EC 4.1.2.29) (characterized)
to candidate H281DRAFT_04459 H281DRAFT_04459 5-dehydro-2-deoxygluconokinase
Query= reanno::BFirm:BPHYT_RS13910 (681 letters) >FitnessBrowser__Burk376:H281DRAFT_04459 Length = 682 Score = 1258 bits (3254), Expect = 0.0 Identities = 626/683 (91%), Positives = 651/683 (95%), Gaps = 3/683 (0%) Query: 1 MDQSITSSGVTSASAP--ATGSRFAPGRSRDIVCLGRLAVDLYAQQVGARLEDVSSFAKY 58 M QS T SG T A P A GSRFAPGRSRDIVCLGRLAVDLYAQQVGARLEDVSSFAKY Sbjct: 1 MAQSSTFSG-TPAQQPGGAAGSRFAPGRSRDIVCLGRLAVDLYAQQVGARLEDVSSFAKY 59 Query: 59 LGGSSANIAFGCARLGLASSMLARVGNDHMGRFLTETLTKEGCDVSHVRIDQERLTALVL 118 LGGSSANIAFGCARLGLAS+MLARVGNDHMGRFLTETLTKEGCDVSHVR+D ERLTALVL Sbjct: 60 LGGSSANIAFGCARLGLASAMLARVGNDHMGRFLTETLTKEGCDVSHVRVDPERLTALVL 119 Query: 119 LGLKDRDTFPLIFYRENCADMAVDEADFDEAYIASSKALLITGTHFSTEQVNRTSRRALD 178 LGLKDRDTFPLIFYRENCADMAVDEADFDEA+IASSKALLITGTHFSTEQVNRTSRRALD Sbjct: 120 LGLKDRDTFPLIFYRENCADMAVDEADFDEAFIASSKALLITGTHFSTEQVNRTSRRALD 179 Query: 179 YARRNQVRTVLDIDYRPVLWGLTGKADGETRFVASEGVTAHLQRILPLFDLVIGTEEEFR 238 YARRNQVRTVLDIDYRPVLWGLTGKADGETRFVASEGVTAHLQRILPLFDLVIGTEEEFR Sbjct: 180 YARRNQVRTVLDIDYRPVLWGLTGKADGETRFVASEGVTAHLQRILPLFDLVIGTEEEFR 239 Query: 239 IAGGKTELVDALAMVRAVTPATLVLKRGPMGCQIIDGAVPASLDDLPIHGGVEVEVLNVL 298 IAGGK++L+DALAMVRAVTPATLVLKRGPMGCQIIDG VPASLDD P+HGGVEVEVLNVL Sbjct: 240 IAGGKSDLLDALAMVRAVTPATLVLKRGPMGCQIIDGEVPASLDDTPVHGGVEVEVLNVL 299 Query: 299 GAGDAFASGFLSGWLRDQPLEACARIANASGALVVSRHGCAPAMPTPAELDYFLREAKAD 358 GAGDAFASGFLSGWLRDQPL+ACAR ANASGALVVSRH CAPAMPTPAELDYFLREAKAD Sbjct: 300 GAGDAFASGFLSGWLRDQPLDACARAANASGALVVSRHACAPAMPTPAELDYFLREAKAD 359 Query: 359 PQRMRRPDRDATLARLHRVSPARKQWDEVLGFAFDHRNQFFELAQQTGADDARIARLKGL 418 P+RMRRPDRDATLARLHRV+PARKQWDEVLGFAFDHRNQFFELAQQ+GA +ARIA+LKGL Sbjct: 360 PERMRRPDRDATLARLHRVTPARKQWDEVLGFAFDHRNQFFELAQQSGASEARIAQLKGL 419 Query: 419 FVEAVAQTESALGLQNRIGVLIDDRYGQDALNAATGRGWWIGRPVELPGSVPLVFDHGRS 478 FV+AVAQTESALGLQ RIGVLID RYGQDALNAATGRGWWIGRPVELPGSVPLVFDHGRS Sbjct: 420 FVDAVAQTESALGLQGRIGVLIDSRYGQDALNAATGRGWWIGRPVELPGSVPLVFDHGRS 479 Query: 479 IGTTLIPWPQEHIVKCLVQFHPDEPIEQRLEQEAQLRALYDATQASGHELLLEVIPPKHA 538 +GTTL+ WPQEH+ KCLVQFHPDEP+EQRLEQEAQLRALYDATQASGHELLLEVIPPKHA Sbjct: 480 VGTTLVSWPQEHVAKCLVQFHPDEPVEQRLEQEAQLRALYDATQASGHELLLEVIPPKHA 539 Query: 539 HLPQAPDTVYRALKRLYNIGIYPEWWKLEPMDAAQWRNVDALIAERDPYCRGVVLLGLSA 598 +LPQ PD VYRALKRLYNIGIYPEWWKLEPMDAAQWRN+DALIAERDPYCRGVVLLGLSA Sbjct: 540 NLPQGPDIVYRALKRLYNIGIYPEWWKLEPMDAAQWRNIDALIAERDPYCRGVVLLGLSA 599 Query: 599 AVEQLDEGFRAAAQSATCRGFTVGRTIFHEPSHAWLAGEIGDDELIGRVRRTFETLIASW 658 VEQL+EGFRAAA+S+TCRGFTVGRTIFHEPSHAWLAGEIGDDELI RVRRTFETLIASW Sbjct: 600 GVEQLNEGFRAAARSSTCRGFTVGRTIFHEPSHAWLAGEIGDDELIARVRRTFETLIASW 659 Query: 659 RATRGASAAQSSAPKPVHQEQAA 681 RA R +AA+ A VHQEQAA Sbjct: 660 RAARDTAAAERGASNRVHQEQAA 682 Lambda K H 0.321 0.136 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1527 Number of extensions: 41 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 681 Length of database: 682 Length adjustment: 39 Effective length of query: 642 Effective length of database: 643 Effective search space: 412806 Effective search space used: 412806 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 54 (25.4 bits)
Align candidate H281DRAFT_04459 H281DRAFT_04459 (5-dehydro-2-deoxygluconokinase)
to HMM TIGR04382 (iolC: 5-dehydro-2-deoxygluconokinase (EC 2.7.1.92))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR04382.hmm # target sequence database: /tmp/gapView.17965.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR04382 [M=309] Accession: TIGR04382 Description: myo_inos_iolC_N: 5-dehydro-2-deoxygluconokinase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.5e-122 393.5 0.0 4.7e-122 393.1 0.0 1.2 1 lcl|FitnessBrowser__Burk376:H281DRAFT_04459 H281DRAFT_04459 5-dehydro-2-deox Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Burk376:H281DRAFT_04459 H281DRAFT_04459 5-dehydro-2-deoxygluconokinase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 393.1 0.0 4.7e-122 4.7e-122 2 309 .] 29 353 .. 28 353 .. 0.99 Alignments for each domain: == domain 1 score: 393.1 bits; conditional E-value: 4.7e-122 TIGR04382 2 ldlitiGRvgvDlyaqqigasledvksfakylGGspaNiavgaarlGlktalitkvgddqlGrfvr 67 d++++GR++vDlyaqq+ga+ledv+sfakylGGs+aNia+g+arlGl +a++++vg+d++Grf++ lcl|FitnessBrowser__Burk376:H281DRAFT_04459 29 RDIVCLGRLAVDLYAQQVGARLEDVSSFAKYLGGSSANIAFGCARLGLASAMLARVGNDHMGRFLT 94 69**************************************************************** PP TIGR04382 68 eeleregvdtshvvtdkeartslvlleikdpdefpllfYRenaaDlaltvddvdeeliaeakallv 133 e+l++eg+d+shv d+e++t+lvll++kd+d+fpl+fYRen+aD+a++ +d de++ia++kall+ lcl|FitnessBrowser__Burk376:H281DRAFT_04459 95 ETLTKEGCDVSHVRVDPERLTALVLLGLKDRDTFPLIFYRENCADMAVDEADFDEAFIASSKALLI 160 ****************************************************************** PP TIGR04382 134 sgtalskepsreavlkalelakkagvkvvlDiDYRpvlWk............skeeasaalqlvlk 187 +gt++s+e++++++ +al++a++++v++vlDiDYRpvlW+ ++e ++a+lq++l+ lcl|FitnessBrowser__Burk376:H281DRAFT_04459 161 TGTHFSTEQVNRTSRRALDYARRNQVRTVLDIDYRPVLWGltgkadgetrfvASEGVTAHLQRILP 226 ****************************************************************** PP TIGR04382 188 kvdviiGteeEfeiaagekddeaaakallelgaelvvvKrGeeGslvytkd.....eeevevkgfk 248 ++d++iGteeEf+ia g++d a++ +++++++++v+KrG+ G+ ++++ +++ +g++ lcl|FitnessBrowser__Burk376:H281DRAFT_04459 227 LFDLVIGTEEEFRIAGGKSDLLDALAMVRAVTPATLVLKRGPMGCQIIDGEvpaslDDTPVHGGVE 292 **************************************************99***9999999**** PP TIGR04382 249 vevlkvlGaGDaFasgllygllegedlekalelanAagaivvsrlscaeamptleeleefl 309 vevl+vlGaGDaFasg+l+g+l++++l+ +++ anA+ga+vvsr++ca+ampt +el+ fl lcl|FitnessBrowser__Burk376:H281DRAFT_04459 293 VEVLNVLGAGDAFASGFLSGWLRDQPLDACARAANASGALVVSRHACAPAMPTPAELDYFL 353 **********************************************************996 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (309 nodes) Target sequences: 1 (682 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00 # Mc/sec: 21.24 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory