Alignments for a candidate for iolD in Paraburkholderia bryophila 376MFSha3.1

GapMind for catabolism of small carbon sources

Alignments for a candidate for iolD in Paraburkholderia bryophila 376MFSha3.1

Align 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione acylhydrolase (ring-opening) (EC 3.7.1.22) (characterized)
to candidate H281DRAFT_04458 H281DRAFT_04458 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase

Query= BRENDA::Q9L3I0
         (626 letters)



>FitnessBrowser__Burk376:H281DRAFT_04458
          Length = 661

 Score =  681 bits (1757), Expect = 0.0
 Identities = 361/620 (58%), Positives = 443/620 (71%), Gaps = 13/620 (2%)

Query: 12  GKTIRLTMAQAVAHFLKVQMTIV-DGKKV-PIFGGVWAIFGHGNVAGIGEALYQVREELT 69
           G TIRLT AQA+  +L  Q     DG    P+FGGV+AIFGHGNVAG+GEALYQ R+EL 
Sbjct: 22  GTTIRLTTAQALVRYLAAQRVATEDGSGTEPLFGGVFAIFGHGNVAGMGEALYQHRDELP 81

Query: 70  TYRAHNEQGMAHAAIAYAKANFRTRFMACTSSIGPGALNMVTAAGVAHVNRIPVLFLPGD 129
           T RAHNEQ MAH+AIAYAKA+FR R MA T+SIGPGA N++TAA +AHVNR+PVL LPGD
Sbjct: 82  TLRAHNEQAMAHSAIAYAKAHFRRRMMAVTTSIGPGATNLLTAAALAHVNRLPVLLLPGD 141

Query: 130 VFANRAPDPVLQQIEDSATASVSANDAFRSVSRYFDRITRPEQIITALKRAMQVLTDPLD 189
           +F +RAPDPVLQQ+ED     VSANDAF+ VSRYFDRI  P Q+++AL RA++VLTD   
Sbjct: 142 IFVSRAPDPVLQQVEDFHDGGVSANDAFKPVSRYFDRIMHPAQLLSALPRALRVLTDAAS 201

Query: 190 CGPVTLSLCQDVQAEAYDYPESLFAEKVWTTRRPQPDADELANAIALIKASQKPVIVAGG 249
           CGPVTL+L QDVQA+A+D+P   FA ++ T   P P  DE+  A+A ++ +++P+IVAGG
Sbjct: 202 CGPVTLALPQDVQAQAWDFPADFFAPRIVTFYAPAPRVDEIDAAVARLQRAKRPLIVAGG 261

Query: 250 GVLYSQATKELAAFAEAHGIPVVVSQAGKSAINETHPLALGSVGVTGTSAANAIAEETDL 309
           GVLY +AT  L  FA AHGIPV  +QAGKS++    PL  GS+GVTG+ AANA+A + D 
Sbjct: 262 GVLYGRATDALQRFAAAHGIPVAETQAGKSSLAWDDPLNAGSLGVTGSPAANALARDADC 321

Query: 310 VIAVGTRCQDFTTGSWALFKNDSLKMIGLNIAAYDAVKHDSHPLVADAREGLKALSAGLS 369
           V+A+GTR QDFTTGS  LF      +IG+N  A+DA+KH +  + ADAR  L AL+  L 
Sbjct: 322 VLALGTRLQDFTTGSNTLF--TQADVIGINANAFDALKHRAQVVEADARLALDALAERLQ 379

Query: 370 GWKAPAALAEKAAAEKKIWMEA--AARAMATTNAALPSDAQVIGAVAR--TIGGENTTVL 425
            W+A  A   +A      W +            + LP +  VIGAV R  T    N  V+
Sbjct: 380 DWQADRAWTSRAHELAASWRDTVHTLTHAPQRESVLPYEGDVIGAVQRSSTDSPANDIVV 439

Query: 426 CAAGGLPGELHKLWPATAPGSYHMEYGFSCMGYEIAGGLGAKMARPERDVVVMVGDGSYM 485
           CAAG LPGELHKLW A  PG+YH+EYG+SCMGYEIAGGLG K+ARPER+V+VMVGDGSY+
Sbjct: 440 CAAGTLPGELHKLWRAGTPGAYHVEYGYSCMGYEIAGGLGVKLARPEREVIVMVGDGSYL 499

Query: 486 MMNSELATSVMLGLKLNIIVLDNRGYGCINRLQMGTGGANFNNLLKDSYHEVM--PEIDF 543
           MMNSE+ATSVMLG KL ++VLDNRGYGCINRLQ   GGA FNNLL+DS    +  P+IDF
Sbjct: 500 MMNSEIATSVMLGAKLIVVVLDNRGYGCINRLQQACGGAPFNNLLEDSMQGPLGAPQIDF 559

Query: 544 RAHAESMGAIAVKVASIAELEQALADSRKNDRTSVFVIDTDPLITTEAGGHWWDVAVPEV 603
            AHA ++GA A   A++AELE AL  +R  DRT V  IDTDP  TT  GG WW+VAVPEV
Sbjct: 560 AAHARALGARAEHAANVAELEAALQRARSADRTYVISIDTDPARTTSDGGWWWEVAVPEV 619

Query: 604 SSRSEVNRA---HEAYVKAR 620
           S R+ V  A   ++A + AR
Sbjct: 620 SPRASVREARANYDAQISAR 639


Lambda     K      H
   0.318    0.131    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1053
Number of extensions: 37
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 626
Length of database: 661
Length adjustment: 38
Effective length of query: 588
Effective length of database: 623
Effective search space:   366324
Effective search space used:   366324
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources