GapMind for catabolism of small carbon sources

 

Aligments for a candidate for paaF in Paraburkholderia bryophila 376MFSha3.1

Align Enoyl-CoA hydratase (characterized, see rationale)
to candidate H281DRAFT_02514 H281DRAFT_02514 enoyl-CoA hydratase

Query= uniprot:A0A2Z5MEB0
         (258 letters)



>lcl|FitnessBrowser__Burk376:H281DRAFT_02514 H281DRAFT_02514
           enoyl-CoA hydratase
          Length = 258

 Score =  283 bits (724), Expect = 3e-81
 Identities = 145/250 (58%), Positives = 184/250 (73%)

Query: 9   ETRGRVGLVTLSRPKALNALNDALMDELGVALREFDADDAIGAIVLTGSEKAFAAGADIG 68
           E RGRV LV L+    LN L DALMD+L   L   +AD  + A VLTGS KAFAAGADI 
Sbjct: 9   ERRGRVALVRLASDNPLNPLTDALMDQLIDVLLRNEADPQVHATVLTGSGKAFAAGADIV 68

Query: 69  MMSTYSYMDVYKGDYITRNWETVRSIRKPIIAAVAGFALGGGCELAMMCDMIFAADTAKF 128
            MS   Y + +  DYI R+W+ +R++RKPIIAAV G+ALGGGCELAMMCD++ AAD A F
Sbjct: 69  AMSKLDYANAFSDDYIGRSWDRIRTVRKPIIAAVGGYALGGGCELAMMCDIVIAADDALF 128

Query: 129 GQPEIKLGIMPGAGGTQRLPRAVSKAKAMDLCLTARFMDAAEAERAGLVSRVIPAASLID 188
           GQPEIKLGI+PGAGGTQRLPRAV K+ AM  CLT   + A EA   GLVS+++    LID
Sbjct: 129 GQPEIKLGIVPGAGGTQRLPRAVGKSTAMLACLTGEPLGAQEALAYGLVSKIVARERLID 188

Query: 189 ESIAAGATIAEFPLPAVMMVKESVNRAYETTLAEGVHFERRLFHSLFATEDQKEGMAAFV 248
           E++  G  IA   LP ++ +KE+V+RA+E++L+EG+ FERRLFH+ F+  DQKEGMAAF+
Sbjct: 189 EAMRVGEQIARHSLPVIVAIKEAVDRAFESSLSEGLLFERRLFHAGFSLADQKEGMAAFL 248

Query: 249 EKRKPVFKHR 258
           E+R+  F++R
Sbjct: 249 ERRQAAFQNR 258


Lambda     K      H
   0.322    0.135    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 203
Number of extensions: 5
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 258
Length of database: 258
Length adjustment: 24
Effective length of query: 234
Effective length of database: 234
Effective search space:    54756
Effective search space used:    54756
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory