Align Leucine/isoleucine/valine ABC transporter,ATPase component; EC 3.6.3.- (characterized, see rationale)
to candidate H281DRAFT_01586 H281DRAFT_01586 amino acid/amide ABC transporter ATP-binding protein 1, HAAT family
Query= uniprot:G8ALJ0 (294 letters) >FitnessBrowser__Burk376:H281DRAFT_01586 Length = 269 Score = 154 bits (389), Expect = 2e-42 Identities = 99/258 (38%), Positives = 142/258 (55%), Gaps = 16/258 (6%) Query: 11 LLTVEHLTMRFGGLVAVNDVSFSANNGEITAIIGPNGAGKTTLFNCITGFYTPTVGRLTL 70 LL V L FGGL AV+DVSF+ G++ A++GPNGAGK+T FN + G +P+ G + Sbjct: 3 LLHVSGLCKSFGGLKAVDDVSFTLEAGQLLALLGPNGAGKSTCFNMVNGQLSPSSGSIRF 62 Query: 71 RHADGKEFLLERMPGYRISQKASVARTFQNIRLFGGMSVLENLIVAQHNKLIRASGFSIA 130 DG E + R R + V RTFQ F M+VLEN+ +A ++ + G Sbjct: 63 ---DGHELVGMRP---RDIWRLGVGRTFQIAATFNSMTVLENVQMALVSRERKTFG---- 112 Query: 131 GLLGLPSYTRTEREAVDLAKYWLDRVRLLEFADWEAGNLPYGAQRRLEIARAMCTEPVML 190 + P+ +R EA L L++V + A+ G L YG +R+E+A A+ P +L Sbjct: 113 --VWKPAASRYADEAFAL----LEQVGMAADANRACGVLAYGDVKRVELAIALANRPKLL 166 Query: 191 CLDEPAAGLNPRESGELADLLTYIRDEHKIGVLLIEHDMSVVMTISDHVVVLDYGRKISD 250 +DEP AG+ P+E EL L + E KIGVL EH M VV +D ++VL G+ I++ Sbjct: 167 LMDEPTAGMAPKERNELMALTKRLVTEQKIGVLFTEHSMDVVFAYADRMIVLARGKLIAE 226 Query: 251 GDPAFVKNDPAVIRAYLG 268 GD ++NDP V Y G Sbjct: 227 GDGDTIRNDPRVQEVYFG 244 Lambda K H 0.319 0.137 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 177 Number of extensions: 9 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 294 Length of database: 269 Length adjustment: 26 Effective length of query: 268 Effective length of database: 243 Effective search space: 65124 Effective search space used: 65124 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory