Alignments for a candidate for iolA in Paraburkholderia bryophila 376MFSha3.1

GapMind for catabolism of small carbon sources

Alignments for a candidate for iolA in Paraburkholderia bryophila 376MFSha3.1

Align malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) (characterized)
to candidate H281DRAFT_02373 H281DRAFT_02373 methylmalonate-semialdehyde dehydrogenase [acylating]

Query= reanno::pseudo6_N2E2:Pf6N2E2_515
         (500 letters)



>FitnessBrowser__Burk376:H281DRAFT_02373
          Length = 512

 Score =  767 bits (1980), Expect = 0.0
 Identities = 364/492 (73%), Positives = 423/492 (85%)

Query: 9   HYLNGHVQDHDSTRFSNVFNPATGAVQARVALAEPGTVDAAVASALAAFPAWSEQSSLRR 68
           H++NG   D  S RF +VFNPA G V ARV LA    VDAAVA+A AAFPAWSE + ++R
Sbjct: 21  HFINGKAIDGTSGRFGDVFNPALGDVAARVPLASVAEVDAAVAAAYAAFPAWSETAPIKR 80

Query: 69  SRVMFKFKELLDRHHDELAQIISREHGKVLSDAHGEVTRGIEIVEYACGAPNLLKTDFSD 128
           +RV+FKFKELLDRHHDELA++I+REHGKV SDA GEV RGIE+VE+ACG PNLLKTDF+D
Sbjct: 81  ARVLFKFKELLDRHHDELAELITREHGKVFSDAKGEVMRGIEVVEFACGIPNLLKTDFTD 140

Query: 129 NIGGGIDNWNLRQPLGVCAGVTPFNFPVMVPLWMIPLALVAGNCFILKPSERDPSASLLM 188
            IGGGIDNWNLRQPLGV  G+TPFNFP+MVP WM P+A+  GN F+LKPSERDPSAS  +
Sbjct: 141 QIGGGIDNWNLRQPLGVVVGITPFNFPMMVPCWMFPVAIACGNTFVLKPSERDPSASNRL 200

Query: 189 ARLLTEAGLPDGVFNVVQGDKVAVDALLQHPDIEAISFVGSTPIAEYIHQQGTAHGKRVQ 248
           A LL EAGLPDGVFNVV GDKVAVDALL HPD+ A+SFVGSTPIAEYIH +GT HGKRVQ
Sbjct: 201 AELLKEAGLPDGVFNVVHGDKVAVDALLVHPDVSAVSFVGSTPIAEYIHTEGTKHGKRVQ 260

Query: 249 ALGGAKNHMIVMPDADLDQAADALIGAAYGSAGERCMAISIAVAVGDVGDELIAKLLPRI 308
           ALGGAKNH++VMPDADLDQA DALIGAAYGSAGERCMAIS+AVAVG + DELI +L PR+
Sbjct: 261 ALGGAKNHLVVMPDADLDQAVDALIGAAYGSAGERCMAISVAVAVGHIADELIERLTPRV 320

Query: 309 DQLKIGNGQQPGTDMGPLVTAEHKAKVEGFIDAGVAEGARLIVDGRSFKVPGAEQGFFVG 368
           + LKI NG +   +MGPLVTA H+ KV G+I+AG+AEGA+L+VDGR  +V G E+GFF+G
Sbjct: 321 ESLKIMNGMESAAEMGPLVTAVHREKVVGYIEAGIAEGAKLVVDGRGHRVDGHEKGFFLG 380

Query: 369 ATLFDQVTAEMSIYQQEIFGPVLGIVRVPDFATAVALINAHEFGNGVSCFTRDGGIARAF 428
            TLFD V+ +M IY++EIFGPVL +VRVPDFA+AV LINA++F NGVS FT DGGIARAF
Sbjct: 381 GTLFDDVSTDMKIYREEIFGPVLCVVRVPDFASAVELINANDFANGVSLFTSDGGIARAF 440

Query: 429 ARSIKVGMVGINVPIPVPMAWHSFGGWKRSLFGDHHAYGEEGLRFYSRYKSVMQRWPDSI 488
           +R I++GMVGINVPIPVPMAWHSFGGWK+SLFGDHHAYGEEG+RFY+RYKS+MQRWPDSI
Sbjct: 441 SRQIQIGMVGINVPIPVPMAWHSFGGWKKSLFGDHHAYGEEGVRFYTRYKSIMQRWPDSI 500

Query: 489 AKGPEFSMPTAQ 500
           AKG EF+MP A+
Sbjct: 501 AKGAEFTMPVAK 512


Lambda     K      H
   0.321    0.137    0.415 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 849
Number of extensions: 32
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 500
Length of database: 512
Length adjustment: 34
Effective length of query: 466
Effective length of database: 478
Effective search space:   222748
Effective search space used:   222748
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
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Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources