Align RhaT, component of Rhamnose porter (Richardson et al., 2004) (Transport activity is dependent on rhamnokinase (RhaK; AAQ92412) activity (Richardson and Oresnik, 2007) This could be an example of group translocation!) (characterized)
to candidate H281DRAFT_01057 H281DRAFT_01057 monosaccharide ABC transporter ATP-binding protein, CUT2 family (TC 3.A.1.2.-)
Query= TCDB::Q7BSH4 (512 letters) >FitnessBrowser__Burk376:H281DRAFT_01057 Length = 517 Score = 388 bits (996), Expect = e-112 Identities = 215/501 (42%), Positives = 321/501 (64%), Gaps = 6/501 (1%) Query: 11 DSKTGDAPAILEMRGISQIFPGVKALDNVSIALHPGTVTALIGENGAGKSTLVKILTGIY 70 D GD +LE+ I++ FPGVK+LD VS+ + G + AL+GENGAGKSTL KI+ G+Y Sbjct: 6 DISAGD---LLEVIRITKEFPGVKSLDQVSLGVRAGEIHALVGENGAGKSTLTKIMAGVY 62 Query: 71 RPNEGEILVDGRPTTFASAQAAIDAGVTAIHQETVLFDELTVAENIFLGHAPRTRFRTID 130 P+ GEI G+P + SA A G+ IHQE VLF + TVAENIF G PRTR +D Sbjct: 63 APDSGEIRFAGQPLQWKSAADAKRHGIHVIHQELVLFPQSTVAENIFAGAEPRTRIGVLD 122 Query: 131 WQTMNSRSKALLTALESNIDPTIRLKDLSIAQRHLVAIARALSIEARIVIMDEPTAALSR 190 MN ++ALL L +D R+ +LS+A + +V IA+A++ E R++I+DEPTA ++ Sbjct: 123 HGRMNRDAQALLGELGVRLDSRERVGNLSVADQQMVEIAKAMAGETRVLILDEPTAVIAG 182 Query: 191 KEIDDLFRIVRGLKEQGKAILFISHKFDELYEIADDFVVFPRRSRRPVRGVSRKTPQDEI 250 KE+ LF VR L+ +G AI++ISH+ DE++E+ D V + + V+ T +D++ Sbjct: 183 KEVQLLFARVRALRAKGVAIVYISHRLDEIFELCDRVTVMKDGRKVATQSVA-NTTRDQL 241 Query: 251 VRMMVGRDVENVFP-KIDVAIGGP-VLEIRNYSHRTEFRDISFTLRKGEILGVYGLIGAG 308 VR+MVGR++++++P K +A G P VLE+ D S T+R GEI+G+ G++G+G Sbjct: 242 VRLMVGREMKDIYPDKPILAPGVPNVLEVNGLCVGNRVVDASLTVRAGEIVGIAGMVGSG 301 Query: 309 RSELSQSLFGITKPLSGKMVLEGQEITIHSPQDAIRAGIVYVPEERGRHGLALPMPIFQN 368 R+EL+ +FG + G + + G T +P+ AIR G+ +V E+R GL + + + QN Sbjct: 302 RTELAAGIFGALRAHRGTVDIGGTRRTRMTPRTAIRLGLGFVTEDRKSQGLLMLLDVAQN 361 Query: 369 MTLPSLARTSRRGFLRAANEFALARKYAERLDLRAAALSVPVGTLSGGNQQKVVIGKWLA 428 +T +LA+ SR G +R A E A A+ + A + V TLSGGNQQKV+I +W+ Sbjct: 362 VTATTLAQVSRFGLMRPALERAAAQAAINEYRIAGAHPAGGVATLSGGNQQKVLISRWVR 421 Query: 429 TAPKVIILDEPTKGIDIGSKAAVHGFISELAAEGLSIIMVSSELPEIIGMSDRVLVMKEG 488 +V+ILDEPT+G+D+G+KA ++ + EL + GL I+M+SSEL E+IGMSDRVLVM+EG Sbjct: 422 VCKRVLILDEPTRGVDVGAKAEIYRVMRELTSRGLGILMISSELQEVIGMSDRVLVMREG 481 Query: 489 LSAGIFERAELSPEALVRAAT 509 G +++ ++R AT Sbjct: 482 RINGEVSGEQMTEHDIMRLAT 502 Score = 86.7 bits (213), Expect = 2e-21 Identities = 72/226 (31%), Positives = 104/226 (46%), Gaps = 13/226 (5%) Query: 289 ISFTLRKGEILGVYGLIGAGRSELSQSLFGITKPLSGKMVLEGQEITIHSPQDAIRAGIV 348 +S +R GEI + G GAG+S L++ + G+ P SG++ GQ + S DA R GI Sbjct: 31 VSLGVRAGEIHALVGENGAGKSTLTKIMAGVYAPDSGEIRFAGQPLQWKSAADAKRHGIH 90 Query: 349 YVPEERGRHGLALPMPIFQNMTLPSLARTSRRGFLRAA----NEFALARKYAERLDLRAA 404 + +E + P +R G L + AL + RLD R Sbjct: 91 VIHQEL----VLFPQSTVAENIFAGAEPRTRIGVLDHGRMNRDAQALLGELGVRLDSRER 146 Query: 405 ALSVPVGTLSGGNQQKVVIGKWLATAPKVIILDEPTKGIDIGSKAAVHGFISELAAEGLS 464 VG LS +QQ V I K +A +V+ILDEPT I + + L A+G++ Sbjct: 147 -----VGNLSVADQQMVEIAKAMAGETRVLILDEPTAVIAGKEVQLLFARVRALRAKGVA 201 Query: 465 IIMVSSELPEIIGMSDRVLVMKEGLSAGIFERAELSPEALVRAATG 510 I+ +S L EI + DRV VMK+G A + + LVR G Sbjct: 202 IVYISHRLDEIFELCDRVTVMKDGRKVATQSVANTTRDQLVRLMVG 247 Lambda K H 0.320 0.137 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 619 Number of extensions: 28 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 2 Length of query: 512 Length of database: 517 Length adjustment: 35 Effective length of query: 477 Effective length of database: 482 Effective search space: 229914 Effective search space used: 229914 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory