GapMind for catabolism of small carbon sources

 

Alignments for a candidate for fruD in Paraburkholderia bryophila 376MFSha3.1

Align protein-Npi-phosphohistidine-D-fructose phosphotransferase (subunit 1/2) (EC 2.7.1.202) (characterized)
to candidate H281DRAFT_01929 H281DRAFT_01929 PTS IIA-like nitrogen-regulatory protein PtsN

Query= BRENDA::Q8DWE6
         (150 letters)



>FitnessBrowser__Burk376:H281DRAFT_01929
          Length = 171

 Score = 74.7 bits (182), Expect = 7e-19
 Identities = 48/141 (34%), Positives = 80/141 (56%), Gaps = 3/141 (2%)

Query: 4   ENLIKINLICESQDEVFHYLATLVVDNGYANNTESVVQALKLRESEGTTGMMEGFAIPHA 63
           EN++ + L   S+  VF   A L+ +N       +V   L  RE  G+TG+ EG AIPH 
Sbjct: 31  ENVV-VGLSVTSKKRVFEQ-AGLIFENQNGIARSTVTDNLFARERLGSTGLGEGVAIPHG 88

Query: 64  KDKSIVKPSIAILKLKTGVEWHSMDGQLINNVIALFIPEKEAGTTHLKVLSQIARLLVNK 123
           + K + +P  A ++L   + + S DGQ ++ +I L +PE +A   HL++LS+IA+LL ++
Sbjct: 89  RIKGLKQPLAAFVRLAEPIPFESPDGQPVSLLIFLLVPE-QATQQHLEILSEIAQLLSDR 147

Query: 124 TFKEKIKEADTILELKELLTE 144
             +E++   +    L  LLT+
Sbjct: 148 EARERLHTEEDRESLHRLLTQ 168


Lambda     K      H
   0.315    0.133    0.358 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 46
Number of extensions: 3
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 150
Length of database: 171
Length adjustment: 17
Effective length of query: 133
Effective length of database: 154
Effective search space:    20482
Effective search space used:    20482
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 43 (21.2 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory