GapMind for catabolism of small carbon sources

 

Aligments for a candidate for fruF in Paraburkholderia bryophila 376MFSha3.1

Align Fructose import permease protein FruF (characterized)
to candidate H281DRAFT_04148 H281DRAFT_04148 monosaccharide ABC transporter membrane protein, CUT2 family

Query= SwissProt::Q8G846
         (356 letters)



>lcl|FitnessBrowser__Burk376:H281DRAFT_04148 H281DRAFT_04148
           monosaccharide ABC transporter membrane protein, CUT2
           family
          Length = 335

 Score =  167 bits (424), Expect = 3e-46
 Identities = 100/284 (35%), Positives = 162/284 (57%), Gaps = 12/284 (4%)

Query: 28  FILLVIICTIFQHDFLALSWNSNTGGLAGPLITMLQESARYLMIATGMTLVISTAGIDLS 87
           FI L+++C +    F + S+ S        +  +L++ +   +IA GMT VI T GIDLS
Sbjct: 40  FIGLLVVCIVMV--FASDSFLSGAN-----IENVLRQVSINAIIAVGMTCVILTGGIDLS 92

Query: 88  VGSVMAVAGAAAMQTLSNGMNVWLSILIALAVGLAIGCVNGALVSFLGLQPFITTLIMML 147
           VGSVMA+AG  A   +  GMN   ++ + +AVGL  G  NG  V+F G+ P I TL  M 
Sbjct: 93  VGSVMALAGTLAAGLMVAGMNALAALAVGVAVGLGFGAANGFFVAFAGMPPIIVTLATMG 152

Query: 148 AGRGMAKVITSGENTDASAVAGNEPLKWFANGFILGIPANFVIAVIIVILVGLLCRKTAM 207
             RG+A + T G   D       + + +F +G ILGI A  VI  +I ++  +L  +   
Sbjct: 153 IARGLALIYTGGYPIDGLP----DWVSFFGSGKILGIQAPVVIMAVIYVIAWVLLERMPF 208

Query: 208 GMMIEAVGINQEASRMTGIKPKKILFLVYAISGFLAAIAGLFATASVMRVDVVKTGQDLE 267
           G  + A+G N++A+R++G++  ++  +VY I+G  ++ A +  TA +M       G   E
Sbjct: 209 GRYVYAIGGNEQATRLSGVRVARVKLIVYTIAGLTSSFAAIVLTARLMS-GQPNAGVGFE 267

Query: 268 MYAILAVVIGGTSLLGGKFSLAGSAVGAVIIAMIRKTIITLGVN 311
           + AI AVV+GGTS+ GG+ S+ G+ +GA+++ ++   +  +GVN
Sbjct: 268 LDAIAAVVMGGTSISGGRGSIIGTLIGALLLGVLNNGLNMVGVN 311


Lambda     K      H
   0.325    0.136    0.384 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 346
Number of extensions: 21
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 356
Length of database: 335
Length adjustment: 29
Effective length of query: 327
Effective length of database: 306
Effective search space:   100062
Effective search space used:   100062
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory