Align hydroxyacylglutathione hydrolase (EC 3.1.2.6) (characterized)
to candidate H281DRAFT_04399 H281DRAFT_04399 hydroxyacylglutathione hydrolase
Query= BRENDA::Q8ZRM2 (251 letters) >FitnessBrowser__Burk376:H281DRAFT_04399 Length = 263 Score = 205 bits (522), Expect = 7e-58 Identities = 113/256 (44%), Positives = 151/256 (58%), Gaps = 12/256 (4%) Query: 6 IPAFQDNYIWVLTNDEGRCVIVDPGEAAPVLKAIAEHKWMPEAIFLTHHHHDHVGGVKEL 65 +PAF+DNYIWV++ D V+VDPGEAAPV +A W AI LTHHH DHVGGV +L Sbjct: 9 VPAFEDNYIWVVS-DGYHAVVVDPGEAAPVRAYLARRGWRLSAILLTHHHQDHVGGVADL 67 Query: 66 LQHFPQMTVYGPAETQDKGATHLVGDGDTIRVLGEKFTL--FATPGHTLGHVCYFSR--- 120 L + VYGPA TH + GD + + L PGHT GH+ YF Sbjct: 68 LNG-QAVPVYGPAGEAIGHLTHRLKHGDRVTIAAPALDLSVLEVPGHTSGHIAYFQAADP 126 Query: 121 ---PYLFCGDTLFSGGCGRLFEGTPSQMYQSLMKINSLPDDTLICCAHEYTLANIKFALS 177 P++FCGDTLF+ GCGRLFEGTPSQM SL + +LP T + CAHEYTL+NI+FAL+ Sbjct: 127 GGTPHVFCGDTLFACGCGRLFEGTPSQMLASLDSLAALPGATEVHCAHEYTLSNIRFALA 186 Query: 178 ILPHDSFINEYYRKVKELRVKKQMTLPVILKNERKINLFLRTEDIDLINEINKETILQQP 237 P + + + K +LR +K TLP + +ER +N FLR ++ + + ++ P Sbjct: 187 CDPDNDELLAWRDKASDLRARKVPTLPTTIAHERAVNPFLRADNPAIQTTLREQLHADVP 246 Query: 238 E--ARFAWLRSKKDTF 251 + A F +R K+ F Sbjct: 247 DRLASFTLMREWKNRF 262 Lambda K H 0.323 0.140 0.436 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 249 Number of extensions: 15 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 251 Length of database: 263 Length adjustment: 24 Effective length of query: 227 Effective length of database: 239 Effective search space: 54253 Effective search space used: 54253 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 47 (22.7 bits)
Align candidate H281DRAFT_04399 H281DRAFT_04399 (hydroxyacylglutathione hydrolase)
to HMM TIGR03413 (gloB: hydroxyacylglutathione hydrolase (EC 3.1.2.6))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR03413.hmm # target sequence database: /tmp/gapView.2061765.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR03413 [M=248] Accession: TIGR03413 Description: GSH_gloB: hydroxyacylglutathione hydrolase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 6e-102 326.2 0.0 6.8e-102 326.1 0.0 1.0 1 lcl|FitnessBrowser__Burk376:H281DRAFT_04399 H281DRAFT_04399 hydroxyacylgluta Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Burk376:H281DRAFT_04399 H281DRAFT_04399 hydroxyacylglutathione hydrolase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 326.1 0.0 6.8e-102 6.8e-102 2 248 .] 7 262 .. 6 262 .. 0.97 Alignments for each domain: == domain 1 score: 326.1 bits; conditional E-value: 6.8e-102 TIGR03413 2 iaipalsdNyiwllkdekseavvvDpgeaepvlealeekglkleaillTHhHaDHvggvaellekf 67 +++pa++dNyiw+++d +avvvDpgea+pv ++l+++g++l+aillTHhH+DHvggva+ll+ lcl|FitnessBrowser__Burk376:H281DRAFT_04399 7 VPVPAFEDNYIWVVSDGY-HAVVVDPGEAAPVRAYLARRGWRLSAILLTHHHQDHVGGVADLLNGQ 71 589*************99.*********************************************** PP TIGR03413 68 pvkvvgpaeeripgltkevkegdevel..lelevevlevpGHtlgHiayyleee....kvlFcgDt 127 v+v+gpa e+i +lt+++k+gd+v++ +l+++vlevpGHt+gHiay+++++ +++FcgDt lcl|FitnessBrowser__Burk376:H281DRAFT_04399 72 AVPVYGPAGEAIGHLTHRLKHGDRVTIaaPALDLSVLEVPGHTSGHIAYFQAADpggtPHVFCGDT 137 **************************955578899****************98899999******* PP TIGR03413 128 LfsaGCGrlfegtaeqmleslqklaaLpeetkvycaHEYtlsNlrFalavepenealkerlkevea 193 Lf++GCGrlfegt++qml+sl+ laaLp +t+v+caHEYtlsN+rFala++p+n++l ++ +++++ lcl|FitnessBrowser__Burk376:H281DRAFT_04399 138 LFACGCGRLFEGTPSQMLASLDSLAALPGATEVHCAHEYTLSNIRFALACDPDNDELLAWRDKASD 203 ****************************************************************** PP TIGR03413 194 lrakgkptlPstlaeekatNpFLraeeaevkaalee....ekaeevevfaelRekkdkf 248 lra++ ptlP+t+a+e+a+NpFLra++++++++l+e + ++++ f+ +Re+k++f lcl|FitnessBrowser__Burk376:H281DRAFT_04399 204 LRARKVPTLPTTIAHERAVNPFLRADNPAIQTTLREqlhaDVPDRLASFTLMREWKNRF 262 *******************************9999977767778999**********98 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (248 nodes) Target sequences: 1 (263 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.05 # Mc/sec: 1.20 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory