Align trehalose-specific PTS system, I, HPr, and IIA components (characterized)
to candidate H281DRAFT_01852 H281DRAFT_01852 Phosphocarrier protein HPr/phosphoenolpyruvate--protein phosphotransferase (EC 2.7.3.9)/PTS system IIA component, Glc family (TC 4.A.1)
Query= reanno::pseudo3_N2E3:AO353_15995 (844 letters) >FitnessBrowser__Burk376:H281DRAFT_01852 Length = 851 Score = 760 bits (1963), Expect = 0.0 Identities = 421/842 (50%), Positives = 557/842 (66%), Gaps = 23/842 (2%) Query: 7 LQLLAPLSGVLMPLDHVPDPVFASRVIGDGLCIDPTSQVLCAPLAGVVSNLQHSGHAISI 66 ++L+APLSGV++PL+ VPDPVFA +++GDG+ IDPTS L +PL G V+ L S HA++I Sbjct: 10 IELVAPLSGVMVPLETVPDPVFAQKMVGDGISIDPTSHELLSPLPGKVTQLHSSSHAVTI 69 Query: 67 TDDSGVQVLLHIGLDTVNLKGQGFSALVEQGQRVEAGQPLIEFDADYVALHARSLLTLML 126 T SG++VLLHIGLDTV L+G+GF+ LV++G V GQPLI FD YV A SLLT M+ Sbjct: 70 TGASGLEVLLHIGLDTVLLRGEGFTPLVKEGDTVATGQPLIRFDPVYVGAKAASLLTQMV 129 Query: 127 VVSGEPFSLLTPDSGLVACAQPV-LRLSLGDP--RTVVAQEEGEALFSKPVHLPNPNGLH 183 + +G+ + P GLV A V L + L D + A+ G A+ S V LPNP G+H Sbjct: 130 IANGDRVTRYVPAEGLVTAAGDVALTVELADDTAKDQTARTTG-AIVSDEVTLPNPAGMH 188 Query: 184 ARPAAVFAQAAKGFAASICLHKQQDSANAKSLVAIMALQTVHGDALQVSAVGEDAELAIS 243 ARPAAVF AAK + + I L +SANAKS+V+IM L T GD +++ A G DA A S Sbjct: 189 ARPAAVFVGAAKKYESEIRLLHGSNSANAKSIVSIMGLATKFGDRVRIQATGPDAGEAAS 248 Query: 244 TLAQLLADGCGE------AVTPVAVV---------APVVEAQEVSTKLLRGVCASAGSAF 288 LA+LLA+G GE A P ++ A V AQ L GV AS G A Sbjct: 249 VLARLLAEGSGEKPADAPAFPPTSLAPASGSSGEPAVVKRAQPADVNELTGVSASPGLAV 308 Query: 289 GYVVQVAERTLEMPEFAADQQLERESLERALMHATQALQRLRDNAAGEAQADIFKAHQEL 348 G +VQ ++ +++ E Q ER LE A A Q ++ L+ ++A I AH EL Sbjct: 309 GKIVQFRQQVIDVKEAGESPQRERVRLEAAHHEARQNIEALKAKLTDPSKAQILDAHLEL 368 Query: 349 LEDPSLLEQAQALIAEGKSAAFAWNSATEATATLFKSLGSTLLAERALDLMDVGQRVLKL 408 LEDP L A I+EGKSA FAW +A E A + L + LL ERA D+ DVG+RVL L Sbjct: 369 LEDPDLNGMAIGSISEGKSAGFAWRAAFEQQAATLEKLDNPLLRERAGDVRDVGRRVLAL 428 Query: 409 ILGVPDGVWELPDQAILIAEQLTPSQTAALDTGKVLGFATVGGGATSHVAILARALGLPA 468 + G+ ++P +ILIAE+L+PS TA+LD KVLGF T GGATSHVAILAR+LG+PA Sbjct: 429 LAGMQQAQIDVPAGSILIAEELSPSDTASLDRTKVLGFCTTTGGATSHVAILARSLGIPA 488 Query: 469 VCGLPLQVLSLASGTRVLLDADKGELHLDPAVSVIEQLHAKRQQQRQRHQHELENAARAA 528 +CG+ L LA GT V+LD G L +P+ +E+ + +Q ++ + E A++ A Sbjct: 489 ICGIDEDALQLADGTLVVLDGSHGSLRRNPSAGEVEKARERISRQAEKREEEKLAASKLA 548 Query: 529 VTRDGHHFEVTANVASLAETEQAMSLGAEGIGLLRSEFLYQQRSVAPSHDEQAGTYSAIA 588 +T DGH EV AN+ + E A++ GAEG+GLLRSEFL+ R APS DEQA Y A+A Sbjct: 549 MTADGHRVEVVANIRNAKEARDAVAAGAEGVGLLRSEFLFDDRDTAPSEDEQASEYCAVA 608 Query: 589 RALGPQRNLVVRTLDVGGDKPLAYVPMDSEANPFLGMRGIRLCLERPQLLREQFRAILSS 648 ALG +R LV+RTLD GGDKPL+Y+P+ E NPFLG+RG+R+ L+RP + R Q RAIL + Sbjct: 609 EALGRERPLVIRTLDAGGDKPLSYMPLPKEDNPFLGLRGVRVSLDRPDIFRTQLRAILRA 668 Query: 649 AGLARLHIMLPMVSQLSELRLARLMLEEEALALGLR-ELPKLGIMIEVPAAALMADLFAP 707 A + LH+M PMV+ + E+ A+ +L EEA G R K+G+MIEVPAAAL+A+ A Sbjct: 669 APIGNLHVMFPMVAAIEEVLAAKKILLEEA---GDRANSIKVGVMIEVPAAALIAEPLAR 725 Query: 708 EVDFFSIGTNDLTQYTLAMDRDHPRLASQADSFHPSVLRLIASTVKAAHAHGKWVGVCGA 767 EVDFFSIGTNDLTQYTLAMDR HP+LA QAD+ HP+VLRLI TV+ AH HGKWVGVCG Sbjct: 726 EVDFFSIGTNDLTQYTLAMDRGHPKLARQADALHPAVLRLIGMTVEGAHKHGKWVGVCGG 785 Query: 768 LASETLAVPLLLGLGVDELSVSVPLIPAIKAAIREVELSDCQAIAHQVLGLESAEQVREA 827 +AS+ +AVP+L+GLGVDELSVSVP + +IKA + + ++ + +A +V+ L +A +VR Sbjct: 786 IASDAIAVPVLVGLGVDELSVSVPAVGSIKAQLARLTMAQARQLAAEVVRLGTAAEVRAL 845 Query: 828 LS 829 L+ Sbjct: 846 LA 847 Lambda K H 0.318 0.132 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1575 Number of extensions: 57 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 844 Length of database: 851 Length adjustment: 42 Effective length of query: 802 Effective length of database: 809 Effective search space: 648818 Effective search space used: 648818 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 56 (26.2 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory