Alignments for a candidate for treF in Paraburkholderia bryophila 376MFSha3.1

GapMind for catabolism of small carbon sources

Alignments for a candidate for treF in Paraburkholderia bryophila 376MFSha3.1

Align Trehalase; Alpha,alpha-trehalase; Alpha,alpha-trehalose glucohydrolase; EC 3.2.1.28 (characterized)
to candidate H281DRAFT_00297 H281DRAFT_00297 Glucoamylase (glucan-1,4-alpha-glucosidase), GH15 family

Query= SwissProt::P71741
         (680 letters)



>FitnessBrowser__Burk376:H281DRAFT_00297
          Length = 640

 Score =  372 bits (956), Expect = e-107
 Identities = 241/647 (37%), Positives = 343/647 (53%), Gaps = 43/647 (6%)

Query: 43  SSSPLRNPFP-PIADYAFLSDWETTCLISPAGSVEWLCVPRPDSPSVFGAILDR-SAGHF 100
           S++ +R   P PI DYA + D  T  LIS  GSV+WLC PR DS + F A+L     G +
Sbjct: 7   SNALIRKLMPAPIEDYALIGDGHTAALISREGSVDWLCWPRFDSGACFAALLGTPDNGRW 66

Query: 101 RLGP-YGVSVPSARRYLPGSLIMETTWQTHTGWLIVRDALVMGK-WHDIERRSRTHRRTP 158
            + P     V   RRY   +LI+ET ++T  G + + D +  G  W +            
Sbjct: 67  LICPDTDAPVKITRRYRGETLILETDFETPEGAVTLVDFMPPGNGWSE------------ 114

Query: 159 MDWDAEHILLRTVRCVSGTVELMMSCEPAFDYHRLGATWEYSAEAYGEAIARANTEPDAH 218
                   ++R V    GTV + M     FDY   G +  +      E+  RA   PD  
Sbjct: 115 --------MVRIVVGKRGTVRMKMELVLRFDY---GFSIPWVQRLKNESGIRAIVGPD-- 161

Query: 219 PTLRLTTNLRIGLEGREARARTRMKEGDDVFVALSWT----KHPPPQTYDEAADKMWQTT 274
               L T + +  E  +  A   +KEG+ V  +L++     + PP +    A   + +T 
Sbjct: 162 -NAVLRTPVELVGENMKTVAEFTVKEGERVPFSLAYAASHLRLPPARDPHTA---LARTE 217

Query: 275 ECWRQWINIGNFPDHPWRAYLQRSALTLKGLTYSPTGALLAASTTSLPETPRGERNWDYR 334
             W +W   G+     W   ++RS +TL+ L Y PTG ++AA TTSLPE   G RNWDYR
Sbjct: 218 NHWLEWSARGSIQGR-WAEPIRRSLITLRALAYEPTGGIVAAPTTSLPEQLGGTRNWDYR 276

Query: 335 YAWIRDSTFALWGLYTLGLDREADDFFAFIADVSGANNNERHPLQVMYGVGGERSLVEAE 394
           Y W+RD+T  L  L   G   EA  + +++  V+    ++   LQ+MYG+ GER L E E
Sbjct: 277 YCWLRDATITLLALMRGGYYDEARAWRSWLGRVTAGAPDQ---LQIMYGLAGERRLPEFE 333

Query: 395 LHHLSGYDHARPVRIGNGAYNQRQHDIWGSILDSFYLHAKSREQVPENLWPVLKRQVEEA 454
           +  L GY  A+PVRIGN A  Q Q D++G ++++ +L      Q  +  W V    +   
Sbjct: 334 IDWLPGYQGAKPVRIGNNAVGQLQLDVYGEVMNALHLARVGGLQADDTAWNVQCALLRHL 393

Query: 455 IKHWREPDRGIWEVRGEPQHFTSSKVMCWVALDRGAKLAERQGEKSYAQQWRAIADEIKA 514
              W++PD GIWE RG  QHFT SKVM WVA DR    AE    +    +WRA   +I A
Sbjct: 394 TTIWQQPDEGIWETRGGRQHFTFSKVMAWVAFDRALASAEMFKLEGPLDEWRATRAQIHA 453

Query: 515 DILEHGVD-SRGVFTQRYGDEALDASLLLVVLTRFLPPDDPRVRNTVLAIADELTEDGLV 573
           ++ E   + SR  F Q YG + LDAS+LL+ L  FLPP+DPR++ TV AI  +L  DG V
Sbjct: 454 EVCEKAWNPSRNAFVQAYGSDQLDASVLLMPLVSFLPPNDPRIKGTVEAIERDLMHDGFV 513

Query: 574 LRYRVHETDDGLSGEEGTFTICSFWLVSALVEIGEVGRAKRLCERLLSFASPLLLYAEEI 633
           +RYR  E DDGL   EGTF  CSFW+V  L   G +  A  + ERLL   + + L AEE 
Sbjct: 514 MRYRTTEFDDGLPPGEGTFLACSFWMVDNLALQGRLDEAIAMYERLLGLCNDVGLLAEEY 573

Query: 634 EPRSGRHLGNFPQAFTHLALINAVVHVIRAEEEADSSGMFQPANAPM 680
           +P + R +GNFPQAF+H++L+N  +++++  E+A +    QP++  M
Sbjct: 574 DPAAKRLVGNFPQAFSHVSLVNTGLNLMK-HEQAMARATGQPSHNGM 619


Lambda     K      H
   0.319    0.134    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1349
Number of extensions: 68
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 1
Length of query: 680
Length of database: 640
Length adjustment: 38
Effective length of query: 642
Effective length of database: 602
Effective search space:   386484
Effective search space used:   386484
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources