Alignments for a candidate for treF in Paraburkholderia bryophila 376MFSha3.1

GapMind for catabolism of small carbon sources

Alignments for a candidate for treF in Paraburkholderia bryophila 376MFSha3.1

Align alpha,alpha-trehalase (EC 3.2.1.28) (characterized)
to candidate H281DRAFT_02882 H281DRAFT_02882 alpha,alpha-trehalase

Query= BRENDA::P13482
         (565 letters)



>FitnessBrowser__Burk376:H281DRAFT_02882
          Length = 627

 Score =  506 bits (1304), Expect = e-148
 Identities = 262/546 (47%), Positives = 340/546 (62%), Gaps = 13/546 (2%)

Query: 31  EETPVTPQPPDILLGPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDL 90
           + +PV P PP      L+ DVQ A L+PD KTFAD VP + P  I A Y   + Q GF+L
Sbjct: 79  QASPVVPVPPSEQYPDLYRDVQLAHLYPDSKTFADMVPLAPPAQIAAAYETARQQPGFNL 138

Query: 91  RHFVNVNFTLP-KEGEKYVPPEGQSLREHIDGLWPVLTRSTENTEK-WDSLLPLPEPYVV 148
             FV  NFTLP +  + YV    + +  HID LW VL R  +     W SLLPLP+ Y+V
Sbjct: 139 GDFVKRNFTLPVRMSKSYVSDPNEDVVSHIDTLWNVLRREPDAAASPWSSLLPLPDAYIV 198

Query: 149 PGGRFREVYYWDSYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQP 208
           PG RF E+YYWDSYF MLGL  SGH   V D + NFA  I+ YGHIPNGNR+YYLSRSQP
Sbjct: 199 PGDRFDEIYYWDSYFIMLGLEASGHHAWVVDELKNFATLINRYGHIPNGNRTYYLSRSQP 258

Query: 209 PFFALMVELLAQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNR 268
           PFFA MV L+A+ +GDA   QYLP++Q+EYAYWMDG E L AG   + VV+L DG+LLNR
Sbjct: 259 PFFAQMVRLVAEKDGDAVYAQYLPELQREYAYWMDGSEGLPAGHANRHVVRLADGSLLNR 318

Query: 269 YWDDRDTPRPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLR 328
           YWD+R  PR ES+ EDIAT++  P R A +++R+LR+   +GWDFSSRW  + + L T+ 
Sbjct: 319 YWDERAAPRDESYREDIATSQQTPQRNADDLWRNLRAGGETGWDFSSRWFADGKTLATVD 378

Query: 329 TTSIVPVDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYA 388
            TS+ P+DLN L+  +E+ LA+A +  GD   A      A  R   I + LW+ Q   + 
Sbjct: 379 VTSLAPIDLNCLIVDLERALAKAYRVRGDVTHAENMSQRAATRADTIRRVLWDPQLQAFG 438

Query: 389 DYDLKSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWD 448
           DYD     + ++LTAA ++PLY   A++ +A  +A   +  LL+PGGL TT V +GQQWD
Sbjct: 439 DYDFVHRTLTHRLTAATVYPLYTGVASRQQAKAVAATLQRELLRPGGLATTRVATGQQWD 498

Query: 449 APNGWAPLQWVATEGLQNYGQKEVAMDISWHFL-TNVQHTYDREKKLVEKYDV--STTGT 505
           APNGWAPLQ++A  GL+ Y +  +A  I+  ++ TNV + Y    KLVEKYDV  ++ G 
Sbjct: 499 APNGWAPLQYLAVIGLRRYSEPALAQTIATRWIKTNVSY-YQHTGKLVEKYDVDAASPGV 557

Query: 506 GGGGGEYPLQDGFGWTNGVTLKMLDLI-------CPKEQPCDNVPATRPTVKSATTQPST 558
             GGGEYPLQDGFGWTNGV   +L L         P + P      +     +A    +T
Sbjct: 558 SAGGGEYPLQDGFGWTNGVLRTLLALYPQAAGTSRPSDIPAGPAGVSAAAASAAAASKNT 617

Query: 559 KEAQPT 564
              Q T
Sbjct: 618 HRLQGT 623


Lambda     K      H
   0.315    0.132    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1050
Number of extensions: 39
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 565
Length of database: 627
Length adjustment: 37
Effective length of query: 528
Effective length of database: 590
Effective search space:   311520
Effective search space used:   311520
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources