GapMind for catabolism of small carbon sources

 

Aligments for a candidate for catC in Paraburkholderia bryophila 376MFSha3.1

Align Muconolactone Delta-isomerase; MIase; EC 5.3.3.4 (characterized)
to candidate H281DRAFT_01644 H281DRAFT_01644 muconolactone delta-isomerase

Query= SwissProt::P80573
         (92 letters)



>lcl|FitnessBrowser__Burk376:H281DRAFT_01644 H281DRAFT_01644
          muconolactone delta-isomerase
          Length = 96

 Score =  141 bits (355), Expect = 2e-39
 Identities = 65/92 (70%), Positives = 78/92 (84%)

Query: 1  MLYLVRMDVNLPHDMPAAQADDIKAREKAYAQQLQHEGKWQQLYRVVGEYANYSIFDVGS 60
          ML+ VRMDVN PHD+PA  AD+IKAREKAY+Q+LQ  GKW+ ++R+ GEYANYSIFDV S
Sbjct: 1  MLFHVRMDVNFPHDVPAHVADEIKAREKAYSQELQRSGKWRHIWRLAGEYANYSIFDVDS 60

Query: 61 HDELHTLLSGLPLFPYMKIHVTPLAKHPSSIR 92
          + ELH +L+ LPLFPYMKI VTPL +HPSSIR
Sbjct: 61 NAELHDILTALPLFPYMKISVTPLCRHPSSIR 92


Lambda     K      H
   0.321    0.136    0.413 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 73
Number of extensions: 1
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 92
Length of database: 96
Length adjustment: 10
Effective length of query: 82
Effective length of database: 86
Effective search space:     7052
Effective search space used:     7052
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.0 bits)
S2: 39 (19.6 bits)

Align candidate H281DRAFT_01644 H281DRAFT_01644 (muconolactone delta-isomerase)
to HMM TIGR03221 (catC: muconolactone delta-isomerase (EC 5.3.3.4))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR03221.hmm
# target sequence database:        /tmp/gapView.28316.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR03221  [M=90]
Accession:   TIGR03221
Description: muco_delta: muconolactone delta-isomerase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                    Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                    -----------
    1.8e-52  161.7   0.0      2e-52  161.5   0.0    1.0  1  lcl|FitnessBrowser__Burk376:H281DRAFT_01644  H281DRAFT_01644 muconolactone de


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Burk376:H281DRAFT_01644  H281DRAFT_01644 muconolactone delta-isomerase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  161.5   0.0     2e-52     2e-52       1      90 []       2      91 ..       2      91 .. 0.99

  Alignments for each domain:
  == domain 1  score: 161.5 bits;  conditional E-value: 2e-52
                                    TIGR03221  1 lflvrmdvnlPedlpaekaaelkaeekalaqelqreGkwrhlwrvvGeyanvsifdvesndelhells 68
                                                 lf+vrmdvn+P+d+pa+ a+e+ka+eka++qelqr+Gkwrh+wr++Geyan+sifdv+sn elh++l+
  lcl|FitnessBrowser__Burk376:H281DRAFT_01644  2 LFHVRMDVNFPHDVPAHVADEIKAREKAYSQELQRSGKWRHIWRLAGEYANYSIFDVDSNAELHDILT 69
                                                 8******************************************************************* PP

                                    TIGR03221 69 glPlfpymdievtalarhPsai 90
                                                 +lPlfpym+i+vt+l+rhPs+i
  lcl|FitnessBrowser__Burk376:H281DRAFT_01644 70 ALPLFPYMKISVTPLCRHPSSI 91
                                                 ********************97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (90 nodes)
Target sequences:                          1  (96 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 2.45
//
[ok]

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory