Alignments for a candidate for CAT in Paraburkholderia bryophila 376MFSha3.1

GapMind for catabolism of small carbon sources

Alignments for a candidate for CAT in Paraburkholderia bryophila 376MFSha3.1

Align Plastidic cationic amino acid transporter, CAT, of 582 aas and 14 TMSs (characterized)
to candidate H281DRAFT_02180 H281DRAFT_02180 amino acid/polyamine/organocation transporter, APC superfamily

Query= TCDB::ALD51314.1
         (582 letters)



>FitnessBrowser__Burk376:H281DRAFT_02180
          Length = 463

 Score =  224 bits (570), Expect = 8e-63
 Identities = 138/427 (32%), Positives = 233/427 (54%), Gaps = 25/427 (5%)

Query: 50  MKRSLRWYDLVGFGVGGMVGAGVFVTSGRASSHCAGPAVVLSYAIAGFCALLSAFCYTEF 109
           +K++L   DL   GVG ++G G+FV +G  +   AGPA+++S+ IA      +A  Y EF
Sbjct: 22  LKKALGALDLTFLGVGAIIGTGIFVLTGTGAVQ-AGPALMVSFLIAAVACGFAALAYAEF 80

Query: 110 AVDMPVAGGAFSYIRITFGEFLAFLTGANLIIDYVLSNAAVARSFTGYLCTAL---GIES 166
           A  +PVAG  ++Y   T GE  A++ G +L+++Y L+ +AV+  ++GYL + L   G+  
Sbjct: 81  ASTIPVAGSIYTYSYATLGELAAWIIGWDLMLEYGLATSAVSVGWSGYLQSLLSGFGVSL 140

Query: 167 KLRITV--NGLPDGFNEIDVVAVLVVLALTVIICYSTRESSVLNMVLTVLHIVFIVFVIV 224
            + +T     LP      ++ A LV++A+T ++    RES+ +N ++  + +V ++ VI 
Sbjct: 141 PVALTAAPGALPGHETLFNLPAFLVMMAITALLSVGVRESARINNIMVAIKVVVVLLVIA 200

Query: 225 IGFTRGDTKNFTKAGDSNHASGFFPFGASGVFNGAAMVYLSYIGYDAVSTMAEEVKNPVK 284
           +G       N+           F P G +GVF  AA+++ ++IG+D+VS+ AEEVKNP +
Sbjct: 201 VGVFHVTPANWHP---------FMPNGWNGVFGAAAVMFFAFIGFDSVSSAAEEVKNPKR 251

Query: 285 DIPVGVSGSVILVTVLYCLMAASMSMLLPY-DMIDPDAPFSGAFMGSDGWRWVSNVIGVG 343
           D+P+G+  S+ +  VLY  +AA ++ ++P     +   P S A   + G +WV+  I +G
Sbjct: 252 DLPIGIIASLGVCAVLYVAVAAVVTGIVPSAQFANISHPVSYALQVA-GQKWVAGFIDLG 310

Query: 344 AGFGILTSLLVAMLGQARYMCVIGRSSVVPAWFAKVHPKTSTPVNASAFLGICTAAIALF 403
           A  G+LT +LV   GQ R +  + R  ++PA  ++VHP+ +TP   +  +GI    I   
Sbjct: 311 AVLGMLTVILVMAYGQTRVIFAMSRDGLLPARLSRVHPRFATPFFTTWLVGIFFGLIGAL 370

Query: 404 TDLQILLNLVSIGTLFVFYMVANAVIYKRYVSVGVTNPW--------PTLSYLFCFSLTS 455
             L +L  L++IGTL  F MV+ AV+  R     +   +        P L+   C  L +
Sbjct: 371 VPLNVLAELINIGTLAAFSMVSIAVLVLRKTHPELPRAFRCPGVPVVPVLAVASCVFLMA 430

Query: 456 ILFTLLW 462
            L  + W
Sbjct: 431 NLQAVTW 437


Lambda     K      H
   0.326    0.139    0.423 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 639
Number of extensions: 42
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 582
Length of database: 463
Length adjustment: 35
Effective length of query: 547
Effective length of database: 428
Effective search space:   234116
Effective search space used:   234116
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources