Align D-xylose 1-dehydrogenase (EC 1.1.1.175) (characterized)
to candidate H281DRAFT_06300 H281DRAFT_06300 NAD(P)-dependent dehydrogenase, short-chain alcohol dehydrogenase family
Query= reanno::Korea:Ga0059261_1894 (259 letters) >FitnessBrowser__Burk376:H281DRAFT_06300 Length = 266 Score = 228 bits (581), Expect = 1e-64 Identities = 122/254 (48%), Positives = 154/254 (60%), Gaps = 1/254 (0%) Query: 6 AASAAAVYPSLKGKRVLVTGGGSGIGAGIVEGFARQGADVTFFDIAGAESQLLVERLSAD 65 A SA A YPSL + VL+TGG +GIGA VE FA QGA V FFDI + L + L Sbjct: 12 ANSAFARYPSLVDRTVLITGGATGIGASFVEHFAAQGARVAFFDIDATAGEALADELGDS 71 Query: 66 GHKACFERVDLTDVASLQAVIARLIKGAGGFDILVNNAANDDRHAIDEITEAYWDERLSV 125 HK F DLTDV +LQ IA + G LVNNAAND RH I E+T +D ++V Sbjct: 72 KHKPLFLPCDLTDVDALQKAIADVKAALGPIQALVNNAANDKRHTIGEVTRESFDAGIAV 131 Query: 126 NLKHIFFCAQAVVPAMRARGGGAIVNLGSISWHLGLSDLVLYQTCKAAIEGLTRSLARDL 185 N++H FF AQAV+ M+A G+I+NLGSISW L +Y K+A++GLTR LARDL Sbjct: 132 NIRHQFFAAQAVMEDMKAANSGSIINLGSISWMLKNGGYPVYVMSKSAVQGLTRGLARDL 191 Query: 186 GRDGIRATCVIPGNVRTPRQLK-WYSPEGEAEIVAAQCLDGRLAPEDVAAMVLFLASDDA 244 G IR ++PG V T +Q + W G I QC+D L P+D+A M LFLA+DD+ Sbjct: 192 GHFNIRVNTLVPGWVMTEKQKRLWLDDAGRRAIKEGQCIDAELMPDDLARMALFLAADDS 251 Query: 245 RLVTGHSYFVDAGW 258 R++T VD GW Sbjct: 252 RMITAQDIVVDGGW 265 Lambda K H 0.321 0.137 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 198 Number of extensions: 6 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 259 Length of database: 266 Length adjustment: 25 Effective length of query: 234 Effective length of database: 241 Effective search space: 56394 Effective search space used: 56394 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory