GapMind for catabolism of small carbon sources

 

Protein CCNA_00022 in Caulobacter crescentus NA1000

Annotation: FitnessBrowser__Caulo:CCNA_00022

Length: 176 amino acids

Source: Caulo in FitnessBrowser

Candidate for 4 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
2-deoxy-D-ribose catabolism drdehyd-alpha med 2-deoxy-D-ribose dehydrogenase α subunit (characterized) 55% 99% 168.7 isoquinoline 1-oxidoreductase subunit α (EC 1.3.99.16) 62% 189.1
4-hydroxybenzoate catabolism hcrC med 4-hydroxybenzoyl-CoA reductase, γ subunit (EC 1.3.7.9) (characterized) 45% 93% 126.3 isoquinoline 1-oxidoreductase subunit α (EC 1.3.99.16) 62% 189.1
L-arabinose catabolism aldox-small med Glyceraldehyde dehydrogenase small chain; Glyceraldehyde dehydrogenase subunit C; Glyceraldehyde dehydrogenase subunit gamma; EC 1.2.99.8 (characterized) 41% 88% 125.9 isoquinoline 1-oxidoreductase subunit α (EC 1.3.99.16) 62% 189.1
D-xylose catabolism aldox-small med Glyceraldehyde dehydrogenase small chain; Glyceraldehyde dehydrogenase subunit C; Glyceraldehyde dehydrogenase subunit gamma; EC 1.2.99.8 (characterized) 41% 88% 125.9 isoquinoline 1-oxidoreductase subunit α (EC 1.3.99.16) 62% 189.1

Sequence Analysis Tools

View CCNA_00022 at FitnessBrowser

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MAMTLDINGKPVSVQAAPETPLLWVLRDELGMTGTKFGCGLAQCGACTVHMDGQPIRSCV
TPIEAVGAAKVTTIEGLGGQHPVQQAWVRHDVPQCGYCQSGQIMSACALLAQKPKPSDQD
IDEAMAGNICRCGAYQRIRAAIKDAADFGPPTGEAHADIDEAIVAASGATHRGAGR

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory