Protein CCNA_00865 in Caulobacter crescentus NA1000
Annotation: FitnessBrowser__Caulo:CCNA_00865
Length: 478 amino acids
Source: Caulo in FitnessBrowser
Candidate for 21 steps in catabolism of small carbon sources
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| L-arabinose catabolism | xacF | hi | 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26) (characterized) | 100% | 100% | 940.6 | long-chain-aldehyde dehydrogenase (EC 1.2.1.48) | 39% | 321.6 |
| D-galacturonate catabolism | dopDH | hi | 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26) (characterized) | 100% | 100% | 940.6 | long-chain-aldehyde dehydrogenase (EC 1.2.1.48) | 39% | 321.6 |
| D-glucuronate catabolism | dopDH | hi | 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26) (characterized) | 100% | 100% | 940.6 | long-chain-aldehyde dehydrogenase (EC 1.2.1.48) | 39% | 321.6 |
| D-xylose catabolism | dopDH | hi | 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26) (characterized) | 100% | 100% | 940.6 | long-chain-aldehyde dehydrogenase (EC 1.2.1.48) | 39% | 321.6 |
| L-fucose catabolism | aldA | lo | lactaldehyde dehydrogenase (EC 1.2.1.22); 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26) (characterized) | 37% | 96% | 313.5 | 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26) | 100% | 940.6 |
| L-rhamnose catabolism | aldA | lo | lactaldehyde dehydrogenase (EC 1.2.1.22); 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26) (characterized) | 37% | 96% | 313.5 | 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26) | 100% | 940.6 |
| L-threonine catabolism | aldA | lo | lactaldehyde dehydrogenase (EC 1.2.1.22); 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26) (characterized) | 37% | 96% | 313.5 | 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26) | 100% | 940.6 |
| L-isoleucine catabolism | iolA | lo | methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) (characterized) | 34% | 97% | 287 | 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26) | 100% | 940.6 |
| myo-inositol catabolism | mmsA | lo | methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) (characterized) | 34% | 97% | 287 | 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26) | 100% | 940.6 |
| propionate catabolism | iolA | lo | methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) (characterized) | 34% | 97% | 287 | 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26) | 100% | 940.6 |
| L-threonine catabolism | iolA | lo | methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) (characterized) | 34% | 97% | 287 | 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26) | 100% | 940.6 |
| L-valine catabolism | iolA | lo | methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) (characterized) | 34% | 97% | 287 | 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26) | 100% | 940.6 |
| L-valine catabolism | mmsA | lo | methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) (characterized) | 34% | 97% | 287 | 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26) | 100% | 940.6 |
| L-arginine catabolism | putA | lo | L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88) (characterized) | 34% | 91% | 261.9 | 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26) | 100% | 940.6 |
| L-arginine catabolism | rocA | lo | L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88) (characterized) | 34% | 91% | 261.9 | 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26) | 100% | 940.6 |
| L-citrulline catabolism | putA | lo | L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88) (characterized) | 34% | 91% | 261.9 | 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26) | 100% | 940.6 |
| L-citrulline catabolism | rocA | lo | L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88) (characterized) | 34% | 91% | 261.9 | 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26) | 100% | 940.6 |
| L-proline catabolism | putA | lo | L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88) (characterized) | 34% | 91% | 261.9 | 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26) | 100% | 940.6 |
| L-lysine catabolism | amaB | lo | Alpha-aminoadipic semialdehyde dehydrogenase; Alpha-AASA dehydrogenase; Aldehyde dehydrogenase family 7 member A1; Antiquitin-1; Betaine aldehyde dehydrogenase; Delta1-piperideine-6-carboxylate dehydrogenase; P6c dehydrogenase; EC 1.2.1.31; EC 1.2.1.3; EC 1.2.1.8 (characterized) | 33% | 85% | 238.4 | 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26) | 100% | 940.6 |
| L-arginine catabolism | astD | lo | N-succinylglutamate 5-semialdehyde dehydrogenase; EC 1.2.1.71; Succinylglutamic semialdehyde dehydrogenase; SGSD (uncharacterized) | 34% | 96% | 225.7 | 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26) | 100% | 940.6 |
| L-citrulline catabolism | astD | lo | N-succinylglutamate 5-semialdehyde dehydrogenase; EC 1.2.1.71; Succinylglutamic semialdehyde dehydrogenase; SGSD (uncharacterized) | 34% | 96% | 225.7 | 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26) | 100% | 940.6 |
Sequence Analysis Tools
View CCNA_00865 at FitnessBrowser
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
Sequence
MTDTLRHYIGGERVAADAPAESLNPSNTNDVVAKVPMGGQAEVDAAVDAARKAFPAWADA
SPEVRSDLLDKVGSTIIARSADIGRLLAREEGKTLAEGIGETVRAGRIFKYFAGEALRRH
GQNLESTRPGVEIQTYRQAVGVYGLITPWNFPIAIPAWKAAPALAFGNTVVIKPAGPTPA
TANVLADIMAECGAPAGVFNMLFGRGSMGDALIKHKDVDGVSFTGSQGVGAQVAAAAVAR
QARVQLEMGGKNPLIVLDDADLERAVAIALDGSFFATGQRCTASSRLIVQDGIHDKFVAL
LAEKVAALRVGDALDPNTQIGPAVSEDQMETSYRYIDIAASEGGRVVTGGDRIKLDNPGW
YVRPTLIADTQAGMRINNEEVFGPVASTIRVKSYEEALEIANGVEFGLSAGIATTSLKHA
RHFQRYARAGMTMVNLATAGVDYHVPFGGTKSSSYGAREQGFAAVEFFTQTKTSYSWS
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory