GapMind for catabolism of small carbon sources

 

Aligments for a candidate for kgtP in Caulobacter crescentus NA1000

Align Alpha-ketoglutarate permease (characterized)
to candidate CCNA_01071 CCNA_01071 alpha-ketoglutarate permease

Query= SwissProt::P0AEX3
         (432 letters)



>lcl|FitnessBrowser__Caulo:CCNA_01071 CCNA_01071 alpha-ketoglutarate
           permease
          Length = 477

 Score =  395 bits (1015), Expect = e-114
 Identities = 209/470 (44%), Positives = 293/470 (62%), Gaps = 47/470 (10%)

Query: 6   VTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFCSLYFAHIFFPSGNTTTQLLQT 65
           +TA ++ T      R  AI+G S+GNLVEW+D++ Y+  +LYFA  FFP G+ T QLLQ 
Sbjct: 1   MTATTEATRLTPVARARAILGGSAGNLVEWYDWFAYAAFTLYFAPAFFPKGDQTVQLLQA 60

Query: 66  AGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCMMCFGSLVIACLPGYETIGTWAPA 125
           A VF  GF+ RPIG W+ G  AD  GR+ ++ +SV +MC G+ +IA  P ++TIG WAPA
Sbjct: 61  AAVFFLGFVARPIGAWIMGLYADHAGRRAALSVSVSLMCAGAFIIAITPDFQTIGLWAPA 120

Query: 126 LLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGFYASFQYVTLIGGQLLALLVVVVLQH 185
           +LL AR+ QGLSVGGEYG SATYMSE+A + R+GF++SF YVTLI GQLLAL V+++LQ 
Sbjct: 121 ILLFARVLQGLSVGGEYGASATYMSEMAGKARRGFWSSFHYVTLIAGQLLALGVLIILQR 180

Query: 186 TMEDAALREWGWRIPFALGAVLAVVALWLRRQLDET------SQQETRA----------- 228
           T+ + AL+EW WR+PF +GA+LAVV  W+RR L+E+      +Q ET +           
Sbjct: 181 TLGEEALKEWAWRVPFIIGALLAVVVFWIRRGLEESVSFKRPAQSETLSRGQIISAGAFL 240

Query: 229 ---------------LKEAGSLKG---------------LWRNRRAFIMVLGFTAAGSLC 258
                          L +AG   G               + R+ R  ++++G TA GSL 
Sbjct: 241 SLTIASGVVGLMGGPLAKAGQYLGAAFLVLFFVSLIVPLIRRHPRESLLIMGLTAGGSLT 300

Query: 259 FYTFTTYMQKYLVNTAGMHANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSL 318
           FY +TTYMQK+LVNTAG     AS I   +L  FML+QPL G LSD+ GR+  ++     
Sbjct: 301 FYVYTTYMQKFLVNTAGFSKAQASEISALSLIAFMLMQPLAGWLSDRFGRKPMLIIAFGG 360

Query: 319 AAIFTVPILSALQNVSSPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSY 378
             +   PI++A+   +S   A  L++  +   S YT+IS ++KAEMFPA++RALGV L Y
Sbjct: 361 GVLTIWPIMTAISQTNSVGVALALILVGVAFQSCYTAISAVVKAEMFPAEIRALGVALPY 420

Query: 379 AVANAIFGGSAEYVALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGK 428
           A+AN +FGG+AE VAL+ K   +E+ F+ YV  +  +  + S++L   G+
Sbjct: 421 ALANVLFGGTAEMVALAFKHENLESTFYVYVAAVMTIGLICSIILKDTGR 470


Lambda     K      H
   0.328    0.138    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 586
Number of extensions: 28
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 432
Length of database: 477
Length adjustment: 33
Effective length of query: 399
Effective length of database: 444
Effective search space:   177156
Effective search space used:   177156
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory