Alignments for a candidate for D-LDH in Caulobacter crescentus NA1000

GapMind for catabolism of small carbon sources

Alignments for a candidate for D-LDH in Caulobacter crescentus NA1000

Align D-2-hydroxyglutarate--pyruvate transhydrogenase DLD2; D-2HG--pyruvate transhydrogenase DLD2; Actin-interacting protein 2; D-lactate dehydrogenase [cytochrome] 2, mitochondrial; D-lactate ferricytochrome C oxidoreductase; D-LCR; EC 1.1.99.40; EC 1.1.2.4 (characterized)
to candidate CCNA_03500 CCNA_03500 FAD/FMN-containing dehydrogenase

Query= SwissProt::P46681
         (530 letters)



>FitnessBrowser__Caulo:CCNA_03500
          Length = 469

 Score =  281 bits (719), Expect = 4e-80
 Identities = 155/446 (34%), Positives = 242/446 (54%), Gaps = 27/446 (6%)

Query: 93  DWMRKYKGQSKLVLRPKSVEKVSLILNYCNDEKIAVVPQGGNTGLVGGSVPIFDELILSL 152
           +W  +++G++ L++ P+S  +V+ ++  C  E +A+ PQGGNTGLV G +P   E++LS 
Sbjct: 35  EWRGRWQGETPLLVTPRSTAEVAAVVGICAAEGVAITPQGGNTGLVAGQIPR-GEILLST 93

Query: 153 ANLNKIRDFDPVSGILKCDAGVILENANNYVMEQNYMFPLDLGAKGSCHVGGVVATNAGG 212
             L  IRD DP+   +  +AGV L  A+    +    F + + ++GSC +GG+++TNAGG
Sbjct: 94  QKLTAIRDVDPIDDAMVLEAGVTLYEAHQQAAKVGRRFTVGVASEGSCTIGGLISTNAGG 153

Query: 213 LRLLRYGSLHGSVLGLEVVMPNGQIVNSMHSMRKDNTGYDLKQLFIGSEGTIGIITGVSI 272
             +LRYG +   VLG+E V+PNG+I N +  +RKDNTGYDLKQL IG+EGT+GI+T  S+
Sbjct: 154 TAVLRYGMMREQVLGIEAVLPNGEIWNGLKRLRKDNTGYDLKQLLIGAEGTLGIVTAASL 213

Query: 273 LTVPKPKAFNVSYLSVESFEDVQKVFVRARQELSEILSAFEFMDAKSQVLAKSQLKDAAF 332
                  +  V+ + + S  +  ++  RA+ E    + AFE M      L    +     
Sbjct: 214 KLQALLASRAVAIVGLASPANAIQLLARAKDETGGAVEAFELMGRLGFELTVRNVPGLRD 273

Query: 333 PLEDEHPFYILIETSGSNKDHDDSKLETFLENVMEEGIVTDGVVAQDETELQNLWKWREM 392
           PL + HP+Y+LIE +       ++ LE  L   +E G++ D  VAQ ET+++  W  RE 
Sbjct: 274 PLPEAHPWYVLIEIASGEPGAAEAALERLLAGALERGLIADAAVAQTETQMKAFWHIREG 333

Query: 393 IPEASQANGGVYKYDVSLPLKDLYSLVEATNARLSEAELVGDSPKPVVGAIGYGHVGDGN 452
                +  G V+K+DVS+P+  +   +   NA + ++        P    + +GHVGDGN
Sbjct: 334 HSAGQKPEGAVWKHDVSVPVSKIPDFIGQANAAIEKS-------FPGTRIVAFGHVGDGN 386

Query: 453 LHLNV------------AVREYNKNIEKTLEPFVYEFVSSKHGSVSAEHGLGFQKKNYIG 500
           +H +V            A RE    I       V++  +   GS+SAEHGLG  K     
Sbjct: 387 VHYDVLQPVGGDGDAHGAQREAGAKI-------VHDITAGFGGSISAEHGLGAMKTAEAL 439

Query: 501 YSKSPEEVKMMKDLKVHYDPNGILNP 526
             K P EV  ++ ++   DP  I+NP
Sbjct: 440 TYKDPIEVAALRAIRGALDPQRIMNP 465


Lambda     K      H
   0.316    0.135    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 527
Number of extensions: 24
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 530
Length of database: 469
Length adjustment: 34
Effective length of query: 496
Effective length of database: 435
Effective search space:   215760
Effective search space used:   215760
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources