Alignments for a candidate for nagF in Caulobacter crescentus NA1000

GapMind for catabolism of small carbon sources

Alignments for a candidate for nagF in Caulobacter crescentus NA1000

Align N-Acetyl-D-Glucosamine phosphotransferase system transporter, component of N-acetyl glucosamine-specific PTS permease, GlcNAc IIBC/GlcNAc I-HPr-IIA (characterized)
to candidate CCNA_00571 CCNA_00571 phosphoenolpyruvate-protein phosphotransferase/phosphocarrier protein HPr/PTS system, glucose-specific IIA subunit

Query= TCDB::Q9HXN5
         (842 letters)



>FitnessBrowser__Caulo:CCNA_00571
          Length = 878

 Score =  683 bits (1762), Expect = 0.0
 Identities = 399/841 (47%), Positives = 518/841 (61%), Gaps = 24/841 (2%)

Query: 6   LALKAPLSGPVMPLNRVPDPVFSSGTLGEGIAIDPLNDCLHAPCAGLVSHLARTRHALSL 65
           L L APL G V  L   PD VF+   LG+G+AIDPL   LHAPC G V  + R RHA++L
Sbjct: 32  LVLTAPLQGWVSALEEAPDAVFAERMLGDGLAIDPLGSTLHAPCDGSVVSVHRARHAVTL 91

Query: 66  RADNGAELLLHVGLDTVQLQGEGFEALVEEGARVIEGQPLLRFDLDRVARGSRSLITVMI 125
           RA NGAE+L+HVGL+TV L GEGFE  VEEG  V  G  L+  DLD +A+ +RSLIT ++
Sbjct: 92  RATNGAEILMHVGLETVALNGEGFEVFVEEGQTVKAGDRLIGLDLDLLAQRARSLITPVV 151

Query: 126 LTNGDGFQ-VRPLTTNPVEVGAPLLQLSPEKAEQRPANPAPGEGSAQRQVRGRARVAHHG 184
           +TNG+ FQ VR      + VG  L++LSP     R A        A  Q+  +  V    
Sbjct: 152 ITNGEAFQIVRRDQDRQIGVGQFLMELSPIAGASRAA-----ASGAADQITRQIIVPLAH 206

Query: 185 GLHARPAALLRKTAQGFSSQAELHFAGQVASVDSLVGIMGLGVAEQDEVEVICRGEDSEA 244
           G+HARPAA + + A+ F+S   L    + A+  S VG+M L +   D ++++  G D++A
Sbjct: 207 GIHARPAARIAQMAKTFASDLTLATGPRRANARSPVGLMSLAIRHGDTIQLLASGPDAQA 266

Query: 245 ALGALL---------AALASATAGAPKDAPRAIAPGEPARPAAVA--GTLAGVCASPGLA 293
           A+ AL           A  +A   + K AP    P EP  PA +   G L GV A+PGLA
Sbjct: 267 AVTALAELIEGGMGEGAPVAAKTSSTKTAPTKTEPSEPPPPAPLPRDGVLKGVPAAPGLA 326

Query: 294 SGPLARLGAISLPADDGRHRPEEQHLALDQALQRVRDDVQGSLQQARLGGDENEAAIFSA 353
            G   RL    +   +       +  AL  AL+ VR      + +A   GD+   AI +A
Sbjct: 327 IGKAVRLSTAEIVVREAGEGVAHEEAALAVALETVR----ARIGKAAETGDKARKAILAA 382

Query: 354 HLALLEDPGLLDAADMLIDQGVGAAHAWHRAIQAQCEILQALGNLLLAERANDLRDLEKR 413
           H A L+DP L   A  LI +G  A  AW RA+    + LQALG+  +AER +DL DLE++
Sbjct: 383 HQAFLDDPELYAGAHRLIAEGKSAGFAWRRAVGGYVQALQALGDRRMAERVDDLIDLERQ 442

Query: 414 VLRVLLGD--TAPLRVPAGAIVAAREITPSDLAPLVDAGAAGLCMAEGGATSHVAILARS 471
           VLR L G+  T     P G+I+ A E+ PS L     A  AG   A GG TSHVAILA +
Sbjct: 443 VLRALSGEEETGAALAP-GSILLADELLPSQLMGADPAALAGFATARGGPTSHVAILAAA 501

Query: 472 KGLPCLVALGAGLLELEEGRQVVLDAGQGRLELSPDARRLEQVALQVAQREEQRRRQQAD 531
            G+P LVA+G  L ++++G  ++LDA  G L ++PDA+ LE     +AQR++++   +A 
Sbjct: 502 MGVPALVAVGGALSKVKDGATLILDADAGTLRVAPDAKALEAAQTALAQRQQRKAAAKAA 561

Query: 532 AQREALTRDGRRIEIGANVASPREAAEAFANGADGVGLLRTEFLFLERRAAPDEEEQRNA 591
           A   A+TRDG RIE+ AN  S  +A  A ANGA+G GLLRTEFLFL+R   PDE+EQ   
Sbjct: 562 AHEPAVTRDGVRIEVFANTGSVADAQAAVANGAEGSGLLRTEFLFLDRETPPDEDEQARQ 621

Query: 592 YQEVLDAMGQRKVIIRTIDVGGDKHLDYLPLPVEENPALGLRGIHLGQARPELLDQQLRA 651
           YQ + +A+  R +IIRT+DVGGDK   YLP+P EENPALGLRG+ +   RP LL  QLRA
Sbjct: 622 YQAIAEALDGRPLIIRTLDVGGDKAAPYLPIPAEENPALGLRGVRVSLWRPHLLKAQLRA 681

Query: 652 LLRVEPLERCRILLPMVSEVDELRAIRRRLGELATQLGIERLPELGVMIEVPSAALLADQ 711
           +LRVEP  +C+I++PMV+ +DELRA+R  L E   +LGI    +LGVMIE P+AA+ AD 
Sbjct: 682 ILRVEPRGQCKIMVPMVASLDELRAVRAVLEEAKRELGITDRVQLGVMIETPAAAVTADL 741

Query: 712 LAEHADFLSIGTNDLSQYALAMDRCHAGLADRIDALHPALLRLIAQTCAGAARHGRWVGV 771
           LA  ADFLSIGTNDL+QY LAMDR +  LA  IDALHPA+LR+I QTC GAARH RWVGV
Sbjct: 742 LAAEADFLSIGTNDLTQYVLAMDRGNPELAAGIDALHPAVLRMIDQTCQGAARHHRWVGV 801

Query: 772 CGALASDPLATPVLVGLGVEELSVGPNLVGEIKTRVRQLDAAECRRHAQALLDLGSARAV 831
           CG LASD  ATP+L+GLGV ELS   ++  ++K RVR L    CR  A   L+  S +AV
Sbjct: 802 CGGLASDLDATPILLGLGVAELSTTASIAPDVKARVRALSLEACRALAAQALEQTSPQAV 861

Query: 832 R 832
           R
Sbjct: 862 R 862


Lambda     K      H
   0.319    0.136    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1842
Number of extensions: 92
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 842
Length of database: 878
Length adjustment: 42
Effective length of query: 800
Effective length of database: 836
Effective search space:   668800
Effective search space used:   668800
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources