Alignments for a candidate for nagF in Caulobacter crescentus NA1000

GapMind for catabolism of small carbon sources

Alignments for a candidate for nagF in Caulobacter crescentus NA1000

Align N-Acetyl-D-Glucosamine phosphotransferase system transporter, component of N-acetyl glucosamine-specific PTS permease, GlcNAc IIBC/GlcNAc I-HPr-IIA (characterized)
to candidate CCNA_00892 CCNA_00892 phosphoenolpyruvate-protein phosphotransferase PtsP

Query= TCDB::Q9HXN5
         (842 letters)



>FitnessBrowser__Caulo:CCNA_00892
          Length = 754

 Score =  250 bits (638), Expect = 2e-70
 Identities = 201/614 (32%), Positives = 291/614 (47%), Gaps = 30/614 (4%)

Query: 231 DEVEVICRGEDSEAALGALLAALASATA--GAPKDAPRAIAPGEPARPAAVAGTLAGVCA 288
           DE EV    ED +  +  +LA + S++   GA +     +AP +P R       L G   
Sbjct: 145 DEEEV----EDLQI-IAMVLAEMVSSSELLGADELKDVELAPHKPER-------LKGSRF 192

Query: 289 SPGLASGPLAR----LGAISLPADDGRHRPEEQHLALDQALQRVRDDVQGSLQQARLGGD 344
           + GLA G        +   +L +DD          A++    ++   ++G   Q  L G 
Sbjct: 193 AEGLAYGVAVLHEQPVAPETLLSDDALAEEARLTYAIEALQTQIDQMLEG---QHGLVGA 249

Query: 345 ENEAAIFSAHLALLEDPGLLDAADMLIDQGVGAAHAWHRAIQAQCEILQALGNLLLAERA 404
             E  +   +     D G   +    +  G+ A  A  R        L    +  L ER 
Sbjct: 250 SYE--VLETYRLFAHDRGWNRSLQEAVRSGLTAEAAVERVRSEHRARLGQARDPYLRERL 307

Query: 405 NDLRDLEKRVLRVLLGDTAPLR-VPAGAIVAAREITPSDLAPLVDAGAAGLCMAEGGATS 463
           +DL DL  R+LR L GD   +R +P  AI+ AR + P+DL         G+ + EG A S
Sbjct: 308 HDLEDLNDRLLRHLSGDVHHVRQLPEDAILIARNLGPADLLEYDRTKLKGILLEEGSAAS 367

Query: 464 HVAILARSKGLPCLVALGAGLLELEEGRQVVLDAGQGRLELSPDARRLEQVALQVAQREE 523
           H  I+AR+  +PC+  L      + EG  VV+DA      L P    ++ +  ++  R +
Sbjct: 368 HAGIVARALDIPCVGRLVGLRDRVNEGDPVVVDAETQEAWLRPRPDVVKALKARMEVRAQ 427

Query: 524 QRRRQQADAQREALTRDGRRIEIGANVASPREAAEAFANGADGVGLLRTEFLFLERRAAP 583
           ++           +T+DG ++ +  N     +       GA+G+GL RTEF F+     P
Sbjct: 428 RKAEFARLRDTPPITKDGSKVTLLMNAGLAVDLDILGETGAEGIGLFRTEFQFMVAEELP 487

Query: 584 DEEEQRNAYQEVLDAMGQRKVIIRTIDVGGDKHLDYLPLPVEENPALGLRGIHLGQARPE 643
             E Q   Y++VLDA     V  RT+D+GGDK L Y+ L  E+NPALG R + +G  RP 
Sbjct: 488 RLEAQTALYEKVLDAANGMPVTFRTLDLGGDKLLPYMELEREDNPALGWRAVRMGLDRPA 547

Query: 644 LLDQQLRALLRVEPLERCRILLPMVSEVDELRAIRRRLG-ELATQLGIERLP----ELGV 698
           LL  Q+RAL++       RI+ P+V+ VDE RA R  +  E+A  L   R      ++G 
Sbjct: 548 LLRMQIRALIKAANGRPLRIMFPLVANVDEFRAARSFVDQEVAWALKRGRPAPARLDVGA 607

Query: 699 MIEVPSAALLADQLAEHADFLSIGTNDLSQYALAMDRCHAGLADRIDALHPALLRLIAQT 758
           MIE PS     D L    DF+S+GTNDL QY  A DR +  ++DR D L PA LR +   
Sbjct: 608 MIEAPSLLWHLDALLPMTDFVSVGTNDLMQYMFAADRGNPRVSDRYDPLSPAALRALKTI 667

Query: 759 CAGAARHGRWVGVCGALASDPLATPVLVGLGVEELSVGPNLVGEIKTRVRQLDAAECRRH 818
               A  G  V VCG +A  PL    LV LG + LS+ P  +G +K  V  LD    RR+
Sbjct: 668 QQACADTGTQVSVCGEMAGRPLEAFALVALGFDRLSMPPAGIGPVKQMVLSLDREAARRN 727

Query: 819 AQALLDLGSARAVR 832
            +ALL  GS  ++R
Sbjct: 728 VEALLK-GSGGSLR 740


Lambda     K      H
   0.319    0.136    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1477
Number of extensions: 74
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 842
Length of database: 754
Length adjustment: 41
Effective length of query: 801
Effective length of database: 713
Effective search space:   571113
Effective search space used:   571113
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources