GapMind for catabolism of small carbon sources

 

Alignments for a candidate for braG in Caulobacter crescentus NA1000

Align ATP-binding component of a broad range amino acid ABC transporter (characterized, see rationale)
to candidate CCNA_03714 CCNA_03714 ABC transporter ATP-binding protein

Query= uniprot:Q1MCU3
         (247 letters)



>FitnessBrowser__Caulo:CCNA_03714
          Length = 252

 Score =  120 bits (302), Expect = 2e-32
 Identities = 73/233 (31%), Positives = 123/233 (52%), Gaps = 4/233 (1%)

Query: 12  LQVNGVETYYGNIRALAGVDVHVNKGEIVSLIGANGAGKSTLMMTICGSPQARTGSVVFE 71
           L V+ V   +G+   +  V + + +GE+  L+G NGAGK+T    + G   A  G++  +
Sbjct: 11  LFVDSVGKSFGDRPVVKNVSLRLKRGEVAGLLGPNGAGKTTCFYMVTGLIAADYGAIYLD 70

Query: 72  GRDITRMPTHEIARLRIAQSPEGRRIFPRMTVLENLQMGAGLDNLKHFAEDVEKIFTLFP 131
           G +IT  P  + ARL +   P+   IF  MTV +N+     +   +   +  E++ ++  
Sbjct: 71  GENITAQPMFQRARLGVGYLPQEASIFRGMTVEQNVMAVVEMRE-RDPRKAREQVTSILE 129

Query: 132 RLKERHAQRGG--TLSGGEQQMLSIGRALMARPKLLLLDEPSLGLAPLIVKGIFEAIRKL 189
            L+  H ++     LSGGE++ + I RAL + P  +LLDEP  G+ PL +  I E I  L
Sbjct: 130 ELRITHIRKSPAVALSGGERRRVEIARALASEPSFMLLDEPFAGIDPLAIADIREVIGYL 189

Query: 190 NEAEGLTVFLVEQNAFAALRLSHRAYVMVNGKVTMSGSGKELLANPEVRAAYL 242
            +  G+ + + + N    L +  RA ++  G+V   GS +E++ NPEV+  YL
Sbjct: 190 -KGRGIGILITDHNVRETLDIIDRASIIHAGEVLFEGSPREIVENPEVKRVYL 241


Lambda     K      H
   0.320    0.137    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 174
Number of extensions: 10
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 247
Length of database: 252
Length adjustment: 24
Effective length of query: 223
Effective length of database: 228
Effective search space:    50844
Effective search space used:    50844
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory