GapMind for catabolism of small carbon sources

 

Aligments for a candidate for gcdH in Caulobacter crescentus NA1000

Align Glutaryl-CoA dehydrogenase, mitochondrial; GCD; EC 1.3.8.6 (characterized)
to candidate CCNA_00436 CCNA_00436 acyl-CoA dehydrogenase, short-chain specific

Query= SwissProt::P81140
         (408 letters)



>lcl|FitnessBrowser__Caulo:CCNA_00436 CCNA_00436 acyl-CoA
           dehydrogenase, short-chain specific
          Length = 382

 Score =  148 bits (373), Expect = 3e-40
 Identities = 115/369 (31%), Positives = 172/369 (46%), Gaps = 16/369 (4%)

Query: 38  DTFRTYCQEHLMPRIVLANRNEVFHREIISEMGELGVLGPTI-KGYGCAGVSSVAYGLLA 96
           DT   +  E L P       N+    ++I EM  LG+ G TI + +G  G++     L+A
Sbjct: 13  DTVARFVAERLRPIEAQVAENDAVPDDVIEEMKGLGLFGLTIPEEFGGLGLTMEEEALVA 72

Query: 97  RELERVDSGYRSAMSVQSSLVMHPIYAYGSEEQQQQKYLPRLAKGELLGCFGLTEPNHGS 156
            EL R    +RS       +    +  +G++EQ+  K+LP +A G ++  F LTEP  GS
Sbjct: 73  IELGRASPAFRSVFGTNVGIGSQGLVMFGNDEQKA-KWLPGIASGAVITSFALTEPEAGS 131

Query: 157 DPGSMETRALHNPSNRSYTLNGAKTWITNSPVADLFVVWARCEDNC-----IRGFLLEKG 211
           D  +++TRA  +  +  Y LNG+K +ITN+  A LF V AR   +      +  FL+ + 
Sbjct: 132 DSAAVQTRATRDGDD--YILNGSKRYITNAGKASLFTVMARTNPDAKGGAGVSAFLVPRD 189

Query: 212 MRGLSAPKIEGKFSLRASATGMIIMDDVEVPEENVL-PKASSLAVPFGCLNNARYGISWG 270
           + GL+  K E K   + +    +  D+V VP  N L  +     V    L+  R  I+  
Sbjct: 190 LPGLTVGKPEKKMGQQGAHIHDVTFDNVRVPAWNRLGAEGEGFKVAMQVLDRGRLHIAAV 249

Query: 271 VLGAAEFCLHTARQYTLDRIQFGVPLAKNQLIQRKLADMLTEITLGLHACLQLGRLKDQD 330
            +G AE  +     Y  +R QFG P+A  QLIQ  +AD  TE        L+  R +D  
Sbjct: 250 CVGVAERLIADCVAYASERKQFGQPIASFQLIQAMIADSKTEALAAKALVLETARKRDAG 309

Query: 331 -KVTPEMVS--LLKRNNCGKALDIARQARDMLGGNGISDEYHVIRHAMNLEAVNTYEGTH 387
             VT E  S  L      G+  D A Q   + GG G   +Y + R   ++     YEGT 
Sbjct: 310 VNVTLEAASSKLFASEMVGRVADRAVQ---VFGGAGYVADYGIERLYRDVRIFRIYEGTS 366

Query: 388 DIHALILGR 396
            +  LI+ R
Sbjct: 367 QVQQLIIAR 375


Lambda     K      H
   0.319    0.136    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 315
Number of extensions: 23
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 408
Length of database: 382
Length adjustment: 31
Effective length of query: 377
Effective length of database: 351
Effective search space:   132327
Effective search space used:   132327
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory