Align ATP-binding cassette domain-containing protein; SubName: Full=Amino acid transporter; SubName: Full=Histidine ABC transporter ATP-binding protein; SubName: Full=Histidine transport system ATP-binding protein (characterized, see rationale)
to candidate CCNA_00294 CCNA_00294 phosphate transport ATP-binding protein pstB
Query= uniprot:A0A1N7U8S3 (276 letters) >FitnessBrowser__Caulo:CCNA_00294 Length = 274 Score = 139 bits (349), Expect = 9e-38 Identities = 102/267 (38%), Positives = 138/267 (51%), Gaps = 22/267 (8%) Query: 18 AQPVTAAIKLQVEGIHKRYGEHEVLKGVSLNARQGDVISLIGASGSGKSTMLRCINFLEQ 77 A P TA K++ G+ YG+ + L V L+ V + IG SG GKST LRCIN + Sbjct: 18 AAPATADPKIKARGVKVFYGDKQALFDVDLDIPAKSVTAFIGPSGCGKSTFLRCINRMND 77 Query: 78 --PDA---GVITLDGISIEMRQGRAGTRAPHQDQLQNLRTRLAMVFQHFNLWSHMTVLEN 132 P A G I +DG A A D + LR+R+ MVFQ N + T+ EN Sbjct: 78 TIPSARVEGSILIDG---------ADVNAKSVDPVV-LRSRVGMVFQKPNPFPK-TIFEN 126 Query: 133 ITMAPR-RVLDVSAAEAEKRARMYLDKVGLPSRVAD---QYPAFLSGGQQQRVAIARALA 188 + PR L AE E L K GL + VAD Q LSGGQQQR+ IARA+A Sbjct: 127 VAYGPRIHGLATGKAELEAIVESSLKKAGLWNEVADRLHQPGTGLSGGQQQRLVIARAIA 186 Query: 189 MEPEIILFDEPTSALDPELVGEVLKVIQTLAEEGRTMLMVTHEMGFARQVSSQVLFLHQG 248 + PE+IL DEP SALDP ++ ++I L + +++VTH M A +VS + F H G Sbjct: 187 VSPEVILMDEPCSALDPIATAKIEELIDELRSQ-FCIVIVTHSMAQAARVSQRTAFFHLG 245 Query: 249 RVEEHGDA-RILDQPNSERLQQFLSNR 274 ++ E G + P R Q +++ R Sbjct: 246 KLVESGPTEEMFTNPRDSRTQDYITGR 272 Lambda K H 0.319 0.133 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 153 Number of extensions: 8 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 276 Length of database: 274 Length adjustment: 25 Effective length of query: 251 Effective length of database: 249 Effective search space: 62499 Effective search space used: 62499 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory