Alignments for a candidate for fruI in Caulobacter crescentus NA1000

GapMind for catabolism of small carbon sources

Alignments for a candidate for fruI in Caulobacter crescentus NA1000

Align Fructose-specific PTS system, I, HPr, and IIA components (characterized)
to candidate CCNA_00892 CCNA_00892 phosphoenolpyruvate-protein phosphotransferase PtsP

Query= reanno::pseudo3_N2E3:AO353_05485
         (953 letters)



>FitnessBrowser__Caulo:CCNA_00892
          Length = 754

 Score =  301 bits (772), Expect = 8e-86
 Identities = 209/606 (34%), Positives = 301/606 (49%), Gaps = 16/606 (2%)

Query: 363 RAIEEGLGEDIEPLPTVSAQ----SEVI--DEITDVVVAPASGCVIQAVAAAPGIAIGPA 416
           R  +E   ED++ +  V A+    SE++  DE+ DV +AP     ++    A G+A G A
Sbjct: 142 RNYDEEEVEDLQIIAMVLAEMVSSSELLGADELKDVELAPHKPERLKGSRFAEGLAYGVA 201

Query: 417 HIQVLQAIDYPLRGESTAIERERLKTSLADVRRDIEGLI--QRSKAKAIREIFITHQEML 474
            +         L  +    E  RL  ++  ++  I+ ++  Q     A  E+  T++   
Sbjct: 202 VLHEQPVAPETLLSDDALAEEARLTYAIEALQTQIDQMLEGQHGLVGASYEVLETYRLFA 261

Query: 475 DDPELTDEVDTRLKQGESAEAAWMAVIDAAARQQESLQDALLAERAADLRDIGRRVLAQL 534
            D      +   ++ G +AEAA   V      +    +D  L ER  DL D+  R+L  L
Sbjct: 262 HDRGWNRSLQEAVRSGLTAEAAVERVRSEHRARLGQARDPYLRERLHDLEDLNDRLLRHL 321

Query: 535 CG-IETPSEPDQPYILVMDEVGPSDVARLDPTRVAGILTARGGATAHSAIVARALGIPAL 593
            G +    +  +  IL+   +GP+D+   D T++ GIL   G A +H+ IVARAL IP +
Sbjct: 322 SGDVHHVRQLPEDAILIARNLGPADLLEYDRTKLKGILLEEGSAASHAGIVARALDIPCV 381

Query: 594 VGAGAAVLRLASGTPLLLDGQRGRLHVDADAATLQRAAEERDNREQRLQAAAAQRHQPAL 653
                   R+  G P+++D +     +      ++      + R QR    A  R  P +
Sbjct: 382 GRLVGLRDRVNEGDPVVVDAETQEAWLRPRPDVVKALKARMEVRAQRKAEFARLRDTPPI 441

Query: 654 TTDGHAVEVFANIGESAGVVSAVEQGAEGIGLLRTELIFMAHQQAPDEATQEVEYRRVLD 713
           T DG  V +  N G +  +    E GAEGIGL RTE  FM  ++ P    Q   Y +VLD
Sbjct: 442 TKDGSKVTLLMNAGLAVDLDILGETGAEGIGLFRTEFQFMVAEELPRLEAQTALYEKVLD 501

Query: 714 GLAGRPLVVRTLDVGGDKPLPYWPIAKEENPFLGVRGIRLTLQRPQIMEAQLRALLRAAD 773
              G P+  RTLD+GGDK LPY  + +E+NP LG R +R+ L RP ++  Q+RAL++AA+
Sbjct: 502 AANGMPVTFRTLDLGGDKLLPYMELEREDNPALGWRAVRMGLDRPALLRMQIRALIKAAN 561

Query: 774 NRPLRIMFPMVGSVDEWRQARDMTER-----LRLEIPV-ADLQLGIMIEVPSAALLAPVL 827
            RPLRIMFP+V +VDE+R AR   ++     L+   P  A L +G MIE PS       L
Sbjct: 562 GRPLRIMFPLVANVDEFRAARSFVDQEVAWALKRGRPAPARLDVGAMIEAPSLLWHLDAL 621

Query: 828 AKEVDFFSVGTNDLTQYTLAIDRGHPTLSAQADGLHPAVLQLIDITVRAAHAHGKWVGVC 887
               DF SVGTNDL QY  A DRG+P +S + D L PA L+ +    +A    G  V VC
Sbjct: 622 LPMTDFVSVGTNDLMQYMFAADRGNPRVSDRYDPLSPAALRALKTIQQACADTGTQVSVC 681

Query: 888 GELAADPLAVPVLVGLGVDELSVSARSIGEVKARVRELSLAQVKHLAQLALAVGSANEVR 947
           GE+A  PL    LV LG D LS+    IG VK  V  L     +   + AL  GS   +R
Sbjct: 682 GEMAGRPLEAFALVALGFDRLSMPPAGIGPVKQMVLSLDREAARRNVE-ALLKGSGGSLR 740

Query: 948 ALVEAL 953
             +E L
Sbjct: 741 GEIETL 746


Lambda     K      H
   0.319    0.135    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1385
Number of extensions: 65
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 953
Length of database: 754
Length adjustment: 42
Effective length of query: 911
Effective length of database: 712
Effective search space:   648632
Effective search space used:   648632
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources