Alignments for a candidate for dgoD in Caulobacter crescentus NA1000

GapMind for catabolism of small carbon sources

Alignments for a candidate for dgoD in Caulobacter crescentus NA1000

Align L-arabinonate dehydratase; ArDHT; D-fuconate dehydratase; Galactonate dehydratase; L-arabonate dehydratase; EC 4.2.1.25; EC 4.2.1.67; EC 4.2.1.6 (characterized)
to candidate CCNA_00862 CCNA_00862 xylonate dehydratase xylD

Query= SwissProt::B5ZZ34
         (579 letters)



>FitnessBrowser__Caulo:CCNA_00862
          Length = 591

 Score =  451 bits (1159), Expect = e-131
 Identities = 242/572 (42%), Positives = 351/572 (61%), Gaps = 16/572 (2%)

Query: 8   PRKLRSQEWYGGTSR---DVIYHRGWLKNQGYPHDLFDGRPVIGILNTWSDMTPCNGHLR 64
           PR+ RS++W+          +Y   ++     P +L  G+P+IGI  T SD++PCN    
Sbjct: 6   PRRFRSRDWFDNPDHIDMTALYLERFMNYGITPEELRSGKPIIGIAQTGSDISPCNRIHL 65

Query: 65  ELAEKVKAGVWEAGGFPLEVPVFSASENTFRPTAMMYRNLAALAVEEAIRGQPMDGCVLL 124
           +L ++V+ G+ +AGG P+E PV    EN  RPTA + RNL+ L + E + G P+D  VL 
Sbjct: 66  DLVQRVRDGIRDAGGIPMEFPVHPIFENCRRPTAALDRNLSYLGLVETLHGYPIDAVVLT 125

Query: 125 VGCDKTTPSLLMGAASCDLPSIVVTGGPMLNGYFRGERVGSGTHLWKFSEMVKAGEMTQA 184
            GCDKTTP+ +M A + ++P+IV++GGPML+G+   E VGSGT +W+    + AGE+T+ 
Sbjct: 126 TGCDKTTPAGIMAATTVNIPAIVLSGGPMLDGWHENELVGSGTVIWRSRRKLAAGEITEE 185

Query: 185 EFLEAEASMSRSSGTCNTMGTASTMASMAEALGMALSGNAAIPGVDSRRKVMAQLTGRRI 244
           EF++  AS + S+G CNTMGTASTM ++AEALG++L+G AAIP     R  MA  TG+RI
Sbjct: 186 EFIDRAASSAPSAGHCNTMGTASTMNAVAEALGLSLTGCAAIPAPYRERGQMAYKTGQRI 245

Query: 245 VQMVKDDLKPSEIMTKQAFENAIRTNAAIGGSTNAVIHLLAIAGRVGIDLSLDDWDRCGR 304
           V +  DD+KP +I+TKQAFENAI   AA GGSTNA  H++A+A   G++++ DDW R   
Sbjct: 246 VDLAYDDVKPLDILTKQAFENAIALVAAAGGSTNAQPHIVAMARHAGVEITADDW-RAAY 304

Query: 305 DVPTIVNLMPSGKYLMEEFFYAGGLPVVLKRLGEAGLLHKDALTVSGETVWDEVKDVVNW 364
           D+P IVN+ P+GKYL E F  AGG P VL  L + G LH D LTV+G+T+ + ++     
Sbjct: 305 DIPLIVNMQPAGKYLGERFHRAGGAPAVLWELLQQGRLHGDVLTVTGKTMSENLQGRETS 364

Query: 365 NEDVILPAEKALTSSGGIVVLRGNLAPKGAVLKPSAASPHLL-----------VHKGRAV 413
           + +VI P  + L    G +VL+GNL    A++K S                  V + RA+
Sbjct: 365 DREVIFPYHEPLAEKAGFLVLKGNLF-DFAIMKSSVIGEEFRKRYLSQPGQEGVFEARAI 423

Query: 414 VFEDIDDYKAKINDDNLDIDENCIMVMKNCGPKGYPGMAEVGNMGLPPKVLKKGILDMVR 473
           VF+  DDY  +IND  L+IDE CI+V++  GP G+PG AEV NM  P  +LKKGI+ +  
Sbjct: 424 VFDGSDDYHKRINDPALEIDERCILVIRGAGPIGWPGSAEVVNMQPPDHLLKKGIMSLPT 483

Query: 474 ISDARMSGTAYGTVVLHTSPEAAVGGPLAVVKNGDMIELDVPNRRLHLDISDEELARRLA 533
           + D R SGTA    +L+ SPE+A+GG L+ ++ GD I +D+   R    + +  +A R  
Sbjct: 484 LGDGRQSGTADSPSILNASPESAIGGGLSWLRTGDTIRIDLNTGRCDALVDEATIAARKQ 543

Query: 534 EWQPNHDLPTSGYAFLHQQHVEGADTGADLDF 565
           +  P      + +  +++ H    DTG  L+F
Sbjct: 544 DGIPAVPATMTPWQEIYRAHASQLDTGGVLEF 575


Lambda     K      H
   0.318    0.135    0.408 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 957
Number of extensions: 57
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 579
Length of database: 591
Length adjustment: 37
Effective length of query: 542
Effective length of database: 554
Effective search space:   300268
Effective search space used:   300268
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources