GapMind for catabolism of small carbon sources

 

Aligments for a candidate for galactonolactonase in Caulobacter crescentus NA1000

Align L-arabinonolactonase (characterized, see rationale)
to candidate CCNA_01882 CCNA_01882 SMP-30/gluconolaconase/LRE-like protein

Query= uniprot:A0A1I2AUG6
         (300 letters)



>lcl|FitnessBrowser__Caulo:CCNA_01882 CCNA_01882
           SMP-30/gluconolaconase/LRE-like protein
          Length = 293

 Score =  149 bits (375), Expect = 1e-40
 Identities = 105/282 (37%), Positives = 136/282 (48%), Gaps = 12/282 (4%)

Query: 13  LGEGILWCEREQALYWTDIQAATLWRHRPADGATRSWEMPERLGCLALCEADGWLLLGLA 72
           LGE  LW      LYW D  A  +WR+ P     RS   P+ +G + L    G L+ GLA
Sbjct: 15  LGESPLWDADAGVLYWVDSMAPAIWRYDPFTSEQRSIPAPKPIGSVVLGRP-GELIAGLA 73

Query: 73  TRLAFFRPEDDLLLPLVSVEPDLPT-RLNDGACDRQGRFVFGTLHEPAAGETRQPIGAFY 131
             +   + +     P+   +   P  R NDG  DRQGRFV GT+   A       IG  Y
Sbjct: 74  DGVYRVQLDTGAFTPIALPDTLAPIERFNDGKADRQGRFVTGTM---AMHNETGRIGKLY 130

Query: 132 RLNADLTLERLNLPGIGISNSVAFSPDGRTMYFCDSPSRVIQCCDYGDRCG---EPRVFA 188
           R +A    E L    I I+NS  FSP G T+YF DS   ++    Y  + G   E R F 
Sbjct: 131 RFSAGGAWEVLPTEPIEIANSTCFSPSGDTLYFADSLRHMVWAFSYDPKTGAVGEKRDFF 190

Query: 189 RVDDERGEPDGSAVDAQGCLWNAQWGLGRVVRYAPDGRVDRIVEVPATQPTRPAFGDSPL 248
                   PDG+ VDA+G +W A     +++R +PDGR+DR+VE PA   + PAFG   L
Sbjct: 191 DTTGFNSAPDGATVDAEGHIWLALVQAQKLIRISPDGRLDRVVESPAPFCSCPAFGGEDL 250

Query: 249 DTLYITSARDGLSSAALATQ-PLAGALFAADA-GASGLPEPR 288
           D LY+TS  D  S   L T    +G L A    G  G+ E R
Sbjct: 251 DILYVTSISD--SGGRLKTDVDASGRLMAFHGLGVRGIAETR 290


Lambda     K      H
   0.321    0.139    0.451 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 305
Number of extensions: 17
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 300
Length of database: 293
Length adjustment: 26
Effective length of query: 274
Effective length of database: 267
Effective search space:    73158
Effective search space used:    73158
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory